TPO1/YLL028W Protein Information Help

Standard Name Tpo1p
Systematic Name Yll028wp
ORF Classification Verified
Description Polyamine transporter of the major facilitator superfamily; member of the 12-spanner drug:H(+) antiporter DHA1 family; recognizes spermine, putrescine, and spermidine; catalyzes uptake of polyamines at alkaline pH and excretion at acidic pH; phosphorylation enhances activity and sorting to the plasma membrane (1, 2, 3, 4)
Name Description Transporter of POlyamines 1
Experimental Data
Molecules/cell 1470 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 586
Molecular Weight (Da) 64,272
Isoelectric Point (pI) 6.86

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tpo1p (InterPro)
Transmembrane Domains There are 12 total predicted transmembrane domains (TMHMM)
Physical Interactions There are 71 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000037312 | Q07824
MIPS: YLL028W
NCBI: 1360210 | 6323000 | 74627235 | NP_013072.1
GenBank/EMBL/DDBJ: DAA09292.1 | Z73133
External Classifications TC: 2.A.1.2 [The Drug:H+ Antiporter-1 (12 Spanner) (DHA1) Family]
Amino Acid Sequence (or in FASTA format)
 
       1  MSDHSPISNK ENHLLPSDSS RSSSSDMHST GTTGTTGVEP VDFTGEGAKY
      51  TTATEGNGGA DLAIQRTTTM NSAAESEVNI TRRLTKILTG SVNEPDRVEV
     101  DYTNCAPMGG DRPYPPSLPS RDLYEVTFDG PNDPLHPFNW PMKKKVLLCL
     151  VLCLDSIAIA MCSSIFASAV PQICEIYHVI EVVAILGITL FVLGFAASPV
     201  IYAPLSELYG RKGVLVLSAF GFALFQFAVA TAENLQTIFI CRFFGGFIGA
     251  APMAVVPAAF ADMFDTNVRG KAIALFSLGV FVGPILSPVM GSYIAQRTTW
     301  RWLEYVVGCF ASAVFVAIVL FFEETHHPTI LVNKAKQMRK QSNNWGIHAA
     351  HEDVELSIKD IVQKTVTRPI IMLFVEPLLL FVTIYNSFVY GILYLLLEAY
     401  PLVFVEGYGF TENGELPYIA LIIGMMVCAA FIWYMDNDYL KRCRAKGKLV
     451  PEARLYAMVI AGTVFPIGIL WFCWTGYYPH KIHWMVPTVG GAFIGFGLMG
     501  IFLPCLNYII ESYLLLAASA VAANTFMRSA FGACFPLFAG YMFRGMGIGW
     551  AGLLLGLFAA AMIPVPLLFL KYGESIRKKS KYAYAA*

external links for Tpo1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Aspergillus (AspGD) BioPIXIE MIPS YeastGFP
Candida (CGD) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
Candida (CandidaDB) Complexome TCDB
YGOB DIP YeastRC Structure Prediction (Seattle)
YOGY GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Tpo1p
1) Tomitori H, et al.  (1999) Identification of a gene for a polyamine transport protein in yeast. J Biol Chem 274(6):3265-7
2) Tomitori H, et al.  (2001) Multiple polyamine transport systems on the vacuolar membrane in yeast. Biochem J 353(Pt 3):681-8
3) Uemura T, et al.  (2005) Characteristics of the polyamine transporter TPO1 and regulation of its activity and cellular localization by phosphorylation. J Biol Chem 280(10):9646-52
4) Dias PJ, et al.  (2010) Evolution of the 12-Spanner Drug:H+ Antiporter DHA1 Family in Hemiascomycetous Yeasts. OMICS 14(6):701-10
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41