KNS1/YLL019C Protein Information Help

Standard Name Kns1p 1
Systematic Name Yll019cp
ORF Classification Verified
Description Protein kinase involved in negative regulation of PolIII transcription; effector kinase of the TOR signaling pathway and phosphorylates Rpc53p to regulate ribosome and tRNA biosynthesis; member of the LAMMER family of protein kinases, which are serine/threonine kinases also capable of phosphorylating tyrosine residues; capable of autophosphorylation (1, 2, 3) Also known as: L124
Name Description Kinase Next to SPA2 1
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 737
Molecular Weight (Da) 83,842
Isoelectric Point (pI) 8.63

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Kns1p (InterPro)
Physical Interactions There are 37 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012DF2E | P32350
MIPS: YLL019C
NCBI: 1297011 | 1360194 | 171793 | 2204255 | 22096360 | 6323009 | NP_013081.1
GenBank/EMBL/DDBJ: DAA09300.1 | M85200 | X97560 | Z73123 | Z73125
External Classifications EC: 2.7.12.1 [Dual-specificity kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSQNIQIGTR KRSRANMNNS TTTGPANNTS SNKTFLDNFE ETRTNKLLDE
      51  MFARQNSFLT DNLRNSLDLN QADNPLRPRQ HQHQLFLDNE NAIELDEEPR
     101  IINTTINNSN NHNSSRVDED ADDDIIFIKE QPIQFSSPLI LPSSSSINNN
     151  NNIVTSNNPG CGTAATSNST YITTPKKFKK QRTISLPQLP LSKLSYQSNY
     201  FNVPDQTNAI VPRVTQTENE LLHLTGSCAK TLEGNKAVNL TIAHSTSPFS
     251  NPPAQIASLP QSNLKKQIGS SLRKFTSNGS SESASSNKSN FKTDKDGHYV
     301  YQENDIFGSG GRFVVKDLLG QGTFGKVLKC IDNKYEPNYV AVKVIRAVDR
     351  YREAAKTELR ILQTILNNDP QGQFQCLLLR ECFDYKNHIC LVTDLYGRSI
     401  YDFMCSNGIA RFPGSHIQAI ARQLIRSVCF LHDLGIIHTD LKPENILICD
     451  ETHIAQKLPL KTVQSLSKRR REASKGKRKI LKNPEIKIID FGSAIFHYEY
     501  HPPVISTRHY RAPEIVLGLG WSFPCDIWSI ACVLVELVIG ESLYPIHENL
     551  EHMAMMQRIN GTPFPTDIID KMFYKSKHKL GNSPSDLNST VIKHFDRKTL
     601  SLQWPEKNKR GDTITTEKSM KRVLQSCDRL DIYISKVLKQ DYGDSLSINW
     651  NLPPEKNWSL INSKLAWKRQ THSSSSSTTD ELDKETFLFW YWFIDLLRKM
     701  FEFDPTKRIT AKDALDHEWF NLGILDDGIA TYNNTQG*

external links for Kns1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA


YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Kns1p
1) Padmanabha R, et al.  (1991) The KNS1 gene of Saccharomyces cerevisiae encodes a nonessential protein kinase homologue that is distantly related to members of the CDC28/cdc2 gene family. Mol Gen Genet 229(1):1-9
2) Lee K, et al.  (1996) Activity and autophosphorylation of LAMMER protein kinases. J Biol Chem 271(44):27299-303
3) Lee J, et al.  (2012) TOR signaling regulates ribosome and tRNA synthesis via LAMMER/Clk and GSK-3 family kinases. Mol Cell 45(6):836-43