PUF3/YLL013C Protein Information Help

Standard Name Puf3p
Systematic Name Yll013cp
ORF Classification Verified
Description Protein of the mitochondrial outer surface, links the Arp2/3 complex with the mitochore during anterograde mitochondrial movement; also binds to and promotes degradation of mRNAs for select nuclear-encoded mitochondrial proteins (1, 2, 3)
Name Description PUmilio-homology domain Family
Experimental Data
Molecules/cell 846 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 879
Molecular Weight (Da) 98,067
Isoelectric Point (pI) 7.21

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Puf3p (InterPro)
Physical Interactions There are 44 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052ED5 | Q07807
MIPS: YLL013C
NCBI: 1297005 | 1360181 | 1495211 | 2204253 | 262118746 | 262118748 | 262118750 | 262118752 | 262118754 | 262118756 | 34922927 | 6323016 | NP_013088.1
GenBank/EMBL/DDBJ: DAA09305.1 | X91488 | X97560 | Z73117 | Z73118
Amino Acid Sequence (or in FASTA format)
 
       1  MEMNMDMDMD MELASIVSSL SALSHSNNNG GQAAAAGIVN GGAAGSQQIG
      51  GFRRSSFTTA NEVDSEILLL HGSSESSPIF KKTALSVGTA PPFSTNSKKF
     101  FGNGGNYYQY RSTDTASLSS ASYNNYHTHH TAANLGKNNK VNHLLGQYSA
     151  SIAGPVYYNG NDNNNSGGEG FFEKFGKSLI DGTRELESQD RPDAVNTQSQ
     201  FISKSVSNAS LDTQNTFEQN VESDKNFNKL NRNTTNSGSL YHSSSNSGSS
     251  ASLESENAHY PKRNIWNVAN TPVFRPSNNP AAVGATNVAL PNQQDGPANN
     301  NFPPYMNGFP PNQFHQGPHY QNFPNYLIGS PSNFISQMIS VQIPANEDTE
     351  DSNGKKKKKA NRPSSVSSPS SPPNNSPFPF AYPNPMMFMP PPPLSAPQQQ
     401  QQQQQQQQQE DQQQQQQQEN PYIYYPTPNP IPVKMPKDEK TFKKRNNKNH
     451  PANNSNNANK QANPYLENSI PTKNTSKKNA SSKSNESTAN NHKSHSHSHP
     501  HSQSLQQQQQ TYHRSPLLEQ LRNSSSDKNS NSNMSLKDIF GHSLEFCKDQ
     551  HGSRFIQREL ATSPASEKEV IFNEIRDDAI ELSNDVFGNY VIQKFFEFGS
     601  KIQKNTLVDQ FKGNMKQLSL QMYACRVIQK ALEYIDSNQR IELVLELSDS
     651  VLQMIKDQNG NHVIQKAIET IPIEKLPFIL SSLTGHIYHL STHSYGCRVI
     701  QRLLEFGSSE DQESILNELK DFIPYLIQDQ YGNYVIQYVL QQDQFTNKEM
     751  VDIKQEIIET VANNVVEYSK HKFASNVVEK SILYGSKNQK DLIISKILPR
     801  DKNHALNLED DSPMILMIKD QFANYVIQKL VNVSEGEGKK LIVIAIRAYL
     851  DKLNKSNSLG NRHLASVEKL AALVENAEV*

external links for Puf3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Puf3p
1) Olivas W and Parker R  (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11
2) Gerber AP, et al.  (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79
3) Garcia-Rodriguez LJ, et al.  (2007) Puf3p, a Pumilio family RNA binding protein, localizes to mitochondria and regulates mitochondrial biogenesis and motility in budding yeast. J Cell Biol 176(2):197-207
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41