MET5/YJR137C Protein Information

Standard Name	Met5p 1
Systematic Name	Yjr137cp
Alias	Ecm17p 2
ORF Classification	Verified
Description	Sulfite reductase beta subunit, involved in amino acid biosynthesis, transcription repressed by methionine (3, 4)
Name Description	METhionine requiring 1

Experimental Data
Molecules/cell	2900 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,442
Molecular Weight (Da)	161,218
Isoelectric Point (pI)	5.24

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Met5p (InterPro)
Physical Interactions	There are 9 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B504 \| P47169 MIPS: YJR137C NCBI: 1015876 \| 1352934 \| 14318438 \| NP_116579.1 GenBank/EMBL/DDBJ: DAA08922.1 \| Z49637
External Classifications	EC: 1.8.1.2 [Sulfite reductase (NADPH)]

Amino Acid Sequence (or in FASTA format)
1 MTASDLLTLP QLLAQYSSSA PQNKVFYTTS TKNSHSSFKG LESVATDATH 51 LLNNQDPLNT IKDQLSKDIL TTVFTDETTL VKSIHHLYSL PNKLPLVITV 101 DLNLQDYSAI PALKDLSFPI LISSDLQTAI SNADSSYKIA TSSLTPVFHF 151 LNLEKIGTST AIEQDIDFPT LEIANEETKV ALSEATDSLT NFELVKGKES 201 ITTVIVNLSP YDAEFSSVLP SNVGLIKIRV YRPWNFSKFL EILPSSVTKI 251 AVLQGVSKKS QSNEFQPFLL DFFGNFNELV SRNIEQVVLT NIGNVNDYGN 301 VINTVISNIN KKEPDNNLFL GESNEKAEEQ AEVTQLISSV KKVVNLEDAY 351 IKVLKQLFSS NLQILNQFSS ETIEPSNPEF GFGRFLKQEA QREELISLAK 401 TSLDPSLYLS EDANKIVQLL SKWLSFNGRD LDEAQLQEAN ATGLEIFQLL 451 QSNQDSSTVL KFLKIAPTSD SFIFKSSWLI GSDAWSYDLG HSGIQQVLSS 501 RKNINVLLID SEPYDHRKQN QDRKKDVGLY AMNYYSAYVA SVAVYASYTQ 551 LLTAIIEASK YNGPSIVLAY LPYNSENDTP LEVLKETKNA VESGYWPLYR 601 FNPVYDDPST DKEAFSLDSS VIRKQLQDFL DRENKLTLLT RKDPSLSRNL 651 KQSAGDALTR KQEKRSKAAF DQLLEGLSGP PLHVYYASDG GNAANLAKRL 701 AARASARGLK ATVLSMDDII LEELPGEENV VFITSTAGQG EFPQDGKSFW 751 EALKNDTDLD LASLNVAVFG LGDSEYWPRK EDKHYFNKPS QDLFKRLELL 801 SAKALIPLGL GDDQDADGFQ TAYSEWEPKL WEALGVSGAA VDDEPKPVTN 851 EDIKRESNFL RGTISENLKD TSSGGVTHAN EQLMKFHGIY TQDDRDIREI 901 RKSQGLEPYY MFMARARLPG GKTTPQQWLA LDHLSDTSGN GTLKLTTRAT 951 FQIHGVLKKN LKHTLRGMNA VLMDTLAAAG DVNRNVMVSA LPTNAKVHQQ 1001 IADMGKLISD HFLPKTTAYH EVWLEGPEEQ DDDPSWPSIF ENRKDGPRKK 1051 KTLVSGNALV DIEPIYGPTY LPRKFKFNIA VPPYNDVDVL SIDVGLVAIV 1101 NPETQIVEGY NVFVGGGMGT THNNKKTYPR LGSCLGFVKT EDIIPPLEGI 1151 VIVQRDHGDR KDRKHARLKY TVDDMGVEGF KQKVEEYWGK KFEPERPFEF 1201 KSNIDYFGWI KDETGLNHFT AFIENGRVED TPDLPQKTGI RKVAEYMLKT 1251 NSGHFRLTGN QHLVISNITD EHVAGIKSIL KTYKLDNTDF SGLRLSSSSC 1301 VGLPTCGLAF AESERFLPDI ITQLEDCLEE YGLRHDSIIM RMTGCPNGCS 1351 RPWLGELALV GKAPHTYNLM LGGGYLGQRL NKLYKANVKD EEIVDYIKPL 1401 FKRYALEREE GEHFGDFCIR VGIIKPTTEG KYFHEDVSED AY*

Amino Acid Sequence (or in FASTA format)

       1  MTASDLLTLP QLLAQYSSSA PQNKVFYTTS TKNSHSSFKG LESVATDATH
      51  LLNNQDPLNT IKDQLSKDIL TTVFTDETTL VKSIHHLYSL PNKLPLVITV
     101  DLNLQDYSAI PALKDLSFPI LISSDLQTAI SNADSSYKIA TSSLTPVFHF
     151  LNLEKIGTST AIEQDIDFPT LEIANEETKV ALSEATDSLT NFELVKGKES
     201  ITTVIVNLSP YDAEFSSVLP SNVGLIKIRV YRPWNFSKFL EILPSSVTKI
     251  AVLQGVSKKS QSNEFQPFLL DFFGNFNELV SRNIEQVVLT NIGNVNDYGN
     301  VINTVISNIN KKEPDNNLFL GESNEKAEEQ AEVTQLISSV KKVVNLEDAY
     351  IKVLKQLFSS NLQILNQFSS ETIEPSNPEF GFGRFLKQEA QREELISLAK
     401  TSLDPSLYLS EDANKIVQLL SKWLSFNGRD LDEAQLQEAN ATGLEIFQLL
     451  QSNQDSSTVL KFLKIAPTSD SFIFKSSWLI GSDAWSYDLG HSGIQQVLSS
     501  RKNINVLLID SEPYDHRKQN QDRKKDVGLY AMNYYSAYVA SVAVYASYTQ
     551  LLTAIIEASK YNGPSIVLAY LPYNSENDTP LEVLKETKNA VESGYWPLYR
     601  FNPVYDDPST DKEAFSLDSS VIRKQLQDFL DRENKLTLLT RKDPSLSRNL
     651  KQSAGDALTR KQEKRSKAAF DQLLEGLSGP PLHVYYASDG GNAANLAKRL
     701  AARASARGLK ATVLSMDDII LEELPGEENV VFITSTAGQG EFPQDGKSFW
     751  EALKNDTDLD LASLNVAVFG LGDSEYWPRK EDKHYFNKPS QDLFKRLELL
     801  SAKALIPLGL GDDQDADGFQ TAYSEWEPKL WEALGVSGAA VDDEPKPVTN
     851  EDIKRESNFL RGTISENLKD TSSGGVTHAN EQLMKFHGIY TQDDRDIREI
     901  RKSQGLEPYY MFMARARLPG GKTTPQQWLA LDHLSDTSGN GTLKLTTRAT
     951  FQIHGVLKKN LKHTLRGMNA VLMDTLAAAG DVNRNVMVSA LPTNAKVHQQ
    1001  IADMGKLISD HFLPKTTAYH EVWLEGPEEQ DDDPSWPSIF ENRKDGPRKK
    1051  KTLVSGNALV DIEPIYGPTY LPRKFKFNIA VPPYNDVDVL SIDVGLVAIV
    1101  NPETQIVEGY NVFVGGGMGT THNNKKTYPR LGSCLGFVKT EDIIPPLEGI
    1151  VIVQRDHGDR KDRKHARLKY TVDDMGVEGF KQKVEEYWGK KFEPERPFEF
    1201  KSNIDYFGWI KDETGLNHFT AFIENGRVED TPDLPQKTGI RKVAEYMLKT
    1251  NSGHFRLTGN QHLVISNITD EHVAGIKSIL KTYKLDNTDF SGLRLSSSSC
    1301  VGLPTCGLAF AESERFLPDI ITQLEDCLEE YGLRHDSIIM RMTGCPNGCS
    1351  RPWLGELALV GKAPHTYNLM LGGGYLGQRL NKLYKANVKD EEIVDYIKPL
    1401  FKRYALEREE GEHFGDFCIR VGIIKPTTEG KYFHEDVSED AY*

external links for Met5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Met5p

1)	Masselot M and De Robichon-Szulmajster H (1975) Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. Mol Gen Genet 139(2):121-32
2)	Lussier M, et al. (1997) Large scale identification of genes involved in cell surface biosynthesis and architecture in Saccharomyces cerevisiae. Genetics 147(2):435-50
3)	Hansen J, et al. (1997) Siroheme biosynthesis in Saccharomyces cerevisiae requires the products of both the MET1 and MET8 genes. FEBS Lett 401(1):20-4
4)	Mountain HA, et al. (1991) Four major transcriptional responses in the methionine/threonine biosynthetic pathway of Saccharomyces cerevisiae. Yeast 7(8):781-803
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41