JSN1/YJR091C Protein Information Help

Standard Name Jsn1p
Systematic Name Yjr091cp
Alias Puf1p
ORF Classification Verified
Description Member of the Puf family of RNA-binding proteins; interacts with mRNAs encoding membrane-associated proteins; involved in localizing the Arp2/3 complex to mitochondria; overexpression causes increased sensitivity to benomyl; JSN1 has a paralog, PUF2, that arose from the whole genome duplication (1, 2, 3, 4, 5)
Name Description Just Say No 1
Experimental Data
Molecules/cell 784 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,091
Molecular Weight (Da) 120,373
Isoelectric Point (pI) 6.96

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Jsn1p (InterPro)
Physical Interactions There are 42 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012DAC9 | P47135
MIPS: YJR091C
NCBI: 1015786 | 1019710 | 1346340 | 6322550 | 974716 | NP_012624.1
GenBank/EMBL/DDBJ: DAA08875.1 | L43493 | L47993 | Z49591
Amino Acid Sequence (or in FASTA format)
 
       1  MDKSKQMNIN NLSNIPEVID PGITIPIYEE EYENNGESNS QLQQQPQKLG
      51  SYRSRAGKFS NTLSNLLPSI SAKLHHSKKN SHGKNGAEFS SSNNSSQSTV
     101  ASKTPRASPS RSKMMESSID GVTMDRPGSL TPPQDMEKLV HFPDSSNNFL
     151  IPAPRGSSDS FNLPHQISRT RNNTMSSQIT SISSIAPKPR TSSGIWSSNA
     201  SANDPMQQHL LQQLQPTTSN NTTNSNTLND YSTKTAYFDN MVSTSGSQMA
     251  DNKMNTNNLA IPNSVWSNTR QRSQSNASSI YTDAPLYEQP ARASISSHYT
     301  IPTQESPLIA DEIDPQSINW VTMDPTVPSI NQISNLLPTN TISISNVFPL
     351  QHQQPQLNNA INLTSTSLAT LCSKYGEVIS ARTLRNLNMA LVEFSSVESA
     401  VKALDSLQGK EVSMIGAPSK ISFAKILPMH QQPPQFLLNS QGLPLGLENN
     451  NLQPQPLLQE QLFNGAVTFQ QQGNVSIPVF NQQSQQSQHQ NHSSGSAGFS
     501  NVLHGYNNNN SMHGNNNNSA NEKEQCPFPL PPPNVNEKED LLREIIELFE
     551  ANSDEYQINS LIKKSLNHKG TSDTQNFGPL PEPLSGREFD PPKLRELRKS
     601  IDSNAFSDLE IEQLAIAMLD ELPELSSDYL GNTIVQKLFE HSSDIIKDIM
     651  LRKTSKYLTS MGVHKNGTWA CQKMITMAHT PRQIMQVTQG VKDYCTPLIN
     701  DQFGNYVIQC VLKFGFPWNQ FIFESIIANF WVIVQNRYGA RAVRACLEAH
     751  DIVTPEQSIV LSAMIVTYAE YLSTNSNGAL LVTWFLDTSV LPNRHSILAP
     801  RLTKRIVELC GHRLASLTIL KVLNYRGDDN ARKIILDSLF GNVNAHDSSP
     851  PKELTKLLCE TNYGPTFVHK VLAMPLLEDD LRAHIIKQVR KVLTDSTQIQ
     901  PSRRLLEEVG LASPSSTHNK TKQQQQQHHN SSISHMFATP DTSGQHMRGL
     951  SVSSVKSGGS KHTTMNTTTT NGSSASTLSP GQPLNANSNS SMGYFSYPGV
    1001  FPVSGFSGNA SNGYAMNNDD LSSQFDMLNF NNGTRLSLPQ LSLTNHNNTT
    1051  MELVNNVGSS QPHTNNNNNN NNTNYNDDNT VFETLTLHSA N*

external links for Jsn1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Jsn1p
1) Machin NA, et al.  (1995) Microtubule stability in budding yeast: characterization and dosage suppression of a benomyl-dependent tubulin mutant. Mol Biol Cell 6(9):1241-59
2) Olivas W and Parker R  (2000) The Puf3 protein is a transcript-specific regulator of mRNA degradation in yeast. EMBO J 19(23):6602-11
3) Gerber AP, et al.  (2004) Extensive association of functionally and cytotopically related mRNAs with Puf family RNA-binding proteins in yeast. PLoS Biol 2(3):E79
4) Fehrenbacher KL, et al.  (2005) A role for Jsn1p in recruiting the Arp2/3 complex to mitochondria in budding yeast. Mol Biol Cell 16(11):5094-102
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41