NPA3/YJR072C Protein Information

Standard Name	Npa3p 1
Systematic Name	Yjr072cp
Alias	Epa1p 2 , Gpn1p 3
ORF Classification	Verified
Description	Member of the conserved GPN-loop GTPase family; has a role in transport of RNA polymerase II to the nucleus; exhibits GTP-dependent binding to PolII; has ATPase activity; involved in sister chromatid cohesion; phosphorylated by the Pcl1p-Pho85p kinase complex; human homolog XAB1 interacts with human RNA polymerase II; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6)
Name Description	Nucleolar Preribosomal Associated 1

Experimental Data
Molecules/cell	15200 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	385
Molecular Weight (Da)	43,194
Isoelectric Point (pI)	4.58

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Npa3p (InterPro)
Physical Interactions	There are 86 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B4B8 \| P47122 MIPS: YJR072C NCBI: 1015754 \| 1019693 \| 1352889 \| 398365155 \| NP_012606.3 \| NM_001181730.3 GenBank/EMBL/DDBJ: DAA08858.1 \| L47993 \| Z49572
External Classifications	EC: 3.6.-.- [Hydrolases, Acting on Acid Anhydrides]

Amino Acid Sequence (or in FASTA format)
1 MSLSTIICIG MAGSGKTTFM QRLNSHLRAE KTPPYVINLD PAVLRVPYGA 51 NIDIRDSIKY KKVMENYQLG PNGAIVTSLN LFSTKIDQVI RLVEQKKDKF 101 QNCIIDTPGQ IECFVWSASG AIITESFASS FPTVIAYIVD TPRNSSPTTF 151 MSNMLYACSI LYKTKLPMIV VFNKTDVCKA DFAKEWMTDF ESFQAAIKED 201 QDLNGDNGLG SGYMSSLVNS MSLMLEEFYS QLDVVGVSSF TGDGFDEFMQ 251 CVDKKVDEYD QYYKQEREKA LNLKKKKEEM RKQKSLNGLM KDLGLNEKSS 301 AAASDNDSID AISDLEEDAN DGLVDRDEDE GVEREYTFPG EERTKGEVNE 351 NSAPDLQRRY QEAMQQVGKT ASSETAENIA KYIRN*

Amino Acid Sequence (or in FASTA format)

       1  MSLSTIICIG MAGSGKTTFM QRLNSHLRAE KTPPYVINLD PAVLRVPYGA
      51  NIDIRDSIKY KKVMENYQLG PNGAIVTSLN LFSTKIDQVI RLVEQKKDKF
     101  QNCIIDTPGQ IECFVWSASG AIITESFASS FPTVIAYIVD TPRNSSPTTF
     151  MSNMLYACSI LYKTKLPMIV VFNKTDVCKA DFAKEWMTDF ESFQAAIKED
     201  QDLNGDNGLG SGYMSSLVNS MSLMLEEFYS QLDVVGVSSF TGDGFDEFMQ
     251  CVDKKVDEYD QYYKQEREKA LNLKKKKEEM RKQKSLNGLM KDLGLNEKSS
     301  AAASDNDSID AISDLEEDAN DGLVDRDEDE GVEREYTFPG EERTKGEVNE
     351  NSAPDLQRRY QEAMQQVGKT ASSETAENIA KYIRN*

external links for Npa3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Npa3p

1)	Dez C, et al. (2004) Npa1p, a component of very early pre-60S ribosomal particles, associates with a subset of small nucleolar RNPs required for peptidyl transferase center modification. Mol Cell Biol 24(14):6324-37
2)	Keniry ME, et al. (2004) The identification of Pcl1-interacting proteins that genetically interact with Cla4 may indicate a link between G1 progression and mitotic exit. Genetics 166(3):1177-86
3)	Alonso B, et al. (2011) A role for GPN-loop GTPase yGPN1 in sister chromatid cohesion. Cell Cycle 10(11):1828-37
4)	Staresincic L, et al. (2011) GTP-dependent binding and nuclear transport of RNA polymerase II by Npa3 protein. J Biol Chem 286(41):35553-61
5)	Gras S, et al. (2007) Structural insights into a new homodimeric self-activated GTPase family. EMBO Rep 8(6):569-75
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41