LIA1/YJR070C Protein Information

Standard Name	Lia1p 1
Systematic Name	Yjr070cp
Alias	Mmd1p 2
ORF Classification	Verified
Description	Deoxyhypusine hydroxylase; HEAT-repeat containing metalloenzyme that catalyzes hypusine formation; binds to and is required for the modification of Hyp2p (eIF5A); complements S. pombe mmd1 mutants defective in mitochondrial positioning; protein abundance increases in response to DNA replication stress (1, 2, 3, 4)
Name Description	Ligand of eIF5A 1

Experimental Data
Molecules/cell	39900 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	325
Molecular Weight (Da)	36,164
Isoelectric Point (pI)	4.61

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Lia1p (InterPro)
Physical Interactions	There are 23 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013B4B5 \| P47120 MIPS: YJR070C NCBI: 1015751 \| 1019692 \| 1352887 \| 6322531 \| NP_012604.1 GenBank/EMBL/DDBJ: DAA08857.1 \| L47993 \| Z49570
External Classifications	EC: 1.14.99.29 [Deoxyhypusine monooxygenase]

Amino Acid Sequence (or in FASTA format)
1 MSTNFEKHFQ ENVDECTLEQ LRDILVNKSG KTVLANRFRA LFNLKTVAEE 51 FATKPEEAKK AIEYIAESFV NDKSELLKHE VAYVLGQTKN LDAAPTLRHV 101 MLDQNQEPMV RHEAAEALGA LGDKDSLDDL NKAAKEDPHV AVRETCELAI 151 NRINWTHGGA KDKENLQQSL YSSIDPAPPL PLEKDATIPE LQALLNDPKQ 201 PLFQRYRAMF RLRDIGTDEA ILALATGFSA ESSLFKHEIA YVFGQIGSPA 251 AVPSLIEVLG RKEEAPMVRH EAAEALGAIA SPEVVDVLKS YLNDEVDVVR 301 ESCIVALDMY DYENSNELEY APTAN*

Amino Acid Sequence (or in FASTA format)

       1  MSTNFEKHFQ ENVDECTLEQ LRDILVNKSG KTVLANRFRA LFNLKTVAEE
      51  FATKPEEAKK AIEYIAESFV NDKSELLKHE VAYVLGQTKN LDAAPTLRHV
     101  MLDQNQEPMV RHEAAEALGA LGDKDSLDDL NKAAKEDPHV AVRETCELAI
     151  NRINWTHGGA KDKENLQQSL YSSIDPAPPL PLEKDATIPE LQALLNDPKQ
     201  PLFQRYRAMF RLRDIGTDEA ILALATGFSA ESSLFKHEIA YVFGQIGSPA
     251  AVPSLIEVLG RKEEAPMVRH EAAEALGAIA SPEVVDVLKS YLNDEVDVVR
     301  ESCIVALDMY DYENSNELEY APTAN*

external links for Lia1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Lia1p

1)	Thompson GM, et al. (2003) Mapping eIF5A binding sites for Dys1 and Lia1: in vivo evidence for regulation of eIF5A hypusination. FEBS Lett 555(3):464-8
2)	Weir BA and Yaffe MP (2004) Mmd1p, a novel, conserved protein essential for normal mitochondrial morphology and distribution in the fission yeast Schizosaccharomyces pombe. Mol Biol Cell 15(4):1656-65
3)	Park JH, et al. (2006) Molecular cloning, expression, and structural prediction of deoxyhypusine hydroxylase: a HEAT-repeat-containing metalloenzyme. Proc Natl Acad Sci U S A 103(1):51-6
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41