CDC8/YJR057W Protein Information

Standard Name	Cdc8p 1, 2
Systematic Name	Yjr057wp
ORF Classification	Verified
Description	Thymidylate and uridylate kinase, functions in de novo biosynthesis of pyrimidine deoxyribonucleotides; converts dTMP to dTDP and dUMP to dUTP; essential for mitotic and meiotic DNA replication; homologous to S. pombe Tmp1p (2, 3, 4, 5)
Name Description	Cell Division Cycle 6

Experimental Data
Molecules/cell	2980 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	216
Molecular Weight (Da)	24,687
Isoelectric Point (pI)	5.49

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Cdc8p (InterPro)
Physical Interactions	There are 8 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000032EAF \| P00572 MIPS: YJR057W NCBI: 1015725 \| 1019679 \| 1170719 \| 171204 \| 172971 \| 172986 \| 3318768 \| 3318769 \| 3318847 \| 3318848 \| 4558230 \| 4558231 \| 4558232 \| 4558233 \| 4558234 \| 4558235 \| 4558236 \| 4558237 \| 6322517 \| NP_012591.1 GenBank/EMBL/DDBJ: DAA08844.1 \| K01783 \| K02116 \| L47993 \| M15468 \| Z49557
External Classifications	EC: 2.7.4.13 [(Deoxy)nucleoside-phosphate kinase] EC: 2.7.4.9 [dTMP kinase]

Amino Acid Sequence (or in FASTA format)
1 MMGRGKLILI EGLDRTGKTT QCNILYKKLQ PNCKLLKFPE RSTRIGGLIN 51 EYLTDDSFQL SDQAIHLLFS ANRWEIVDKI KKDLLEGKNI VMDRYVYSGV 101 AYSAAKGTNG MDLDWCLQPD VGLLKPDLTL FLSTQDVDNN AEKSGFGDER 151 YETVKFQEKV KQTFMKLLDK EIRKGDESIT IVDVTNKGIQ EVEALIWQIV 201 EPVLSTHIDH DKFSFF*

Amino Acid Sequence (or in FASTA format)

       1  MMGRGKLILI EGLDRTGKTT QCNILYKKLQ PNCKLLKFPE RSTRIGGLIN
      51  EYLTDDSFQL SDQAIHLLFS ANRWEIVDKI KKDLLEGKNI VMDRYVYSGV
     101  AYSAAKGTNG MDLDWCLQPD VGLLKPDLTL FLSTQDVDNN AEKSGFGDER
     151  YETVKFQEKV KQTFMKLLDK EIRKGDESIT IVDVTNKGIQ EVEALIWQIV
     201  EPVLSTHIDH DKFSFF*

external links for Cdc8p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Cdc8p

1)	Kuo CL and Campbell JL (1983) Cloning of Saccharomyces cerevisiae DNA replication genes: isolation of the CDC8 gene and two genes that compensate for the cdc8-1 mutation. Mol Cell Biol 3(10):1730-7
2)	Jong AY, et al. (1984) The CDC8 gene of yeast encodes thymidylate kinase. J Biol Chem 259(17):11052-9
3)	Abaigar LT, et al. (1992) Functional and structural conservation of Schizosaccharomyces pombe dTMP kinase gene. Biochim Biophys Acta 1132(2):222-4
4)	Sclafani RA and Fangman WL (1984) Yeast gene CDC8 encodes thymidylate kinase and is complemented by herpes thymidine kinase gene TK. Proc Natl Acad Sci U S A 81(18):5821-5
5)	Jong AY and Campbell JL (1984) Characterization of Saccharomyces cerevisiae thymidylate kinase, the CDC8 gene product. General properties, kinetic analysis, and subcellular localization. J Biol Chem 259(23):14394-8
6)	Hartwell LH, et al. (1970) Genetic control of the cell-division cycle in yeast. I. Detection of mutants. Proc Natl Acad Sci U S A 66(2):352-9
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41