RAV1/YJR033C Protein Information

Standard Name	Rav1p 1
Systematic Name	Yjr033cp
Alias	Soi3p 2, 3
ORF Classification	Verified
Description	Subunit of the RAVE complex (Rav1p, Rav2p, Skp1p), which promotes assembly of the V-ATPase holoenzyme; required for transport between the early and late endosome/PVC and for localization of TGN membrane proteins; potential Cdc28p substrate (1, 2, 3, 4, 5)
Name Description	Regulator of (H+)-ATPase in Vacuolar membrane 1

Experimental Data
Molecules/cell	149 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,357
Molecular Weight (Da)	154,932
Isoelectric Point (pI)	8.2

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Rav1p (InterPro)
Physical Interactions	There are 32 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005306D \| P47104 MIPS: YJR033C NCBI: 1015679 \| 1129167 \| 1197061 \| 1352875 \| 398364895 \| NP_012567.3 GenBank/EMBL/DDBJ: DAA08822.1 \| L36344 \| X87297 \| Z49533

Amino Acid Sequence (or in FASTA format)
1 MSLNFLPGRP NATPQTACQA TWQNHTIFAY CSGNNLIILT NKFTRLQTIY 51 TQSDCTAVDI NSQNGFIALS FHNRVLIYKP IHQIMQNPKW TQCCQLFHDD 101 TPVNCLRWSS DNELAIGSDF LSFWKIKDNF GVYQPILQWN QKQPKPVYNV 151 IISQDSQLIV SIGKYDCNAK LWKRVSIVGE QAIFNLTMLP HPKPITAMRW 201 KKEPDQVSKN NTASHALYTL CEDKVLRIWS CFEMEKNHTV QIWGEVPLSP 251 TQKFCVIIDN WIIRQTLSVK DSEIFDISDS DIVILGSMTG EMEVLALNNL 301 SQDPPKPMTK KTISHKKVKK ATMLNDTRYL YLPEIQPYDN VKGKLSFLVH 351 DLQGVIRHLL IDILQLINNK TEDLSAALEH KFTGHNKSVQ KLVRSSDGEA 401 LLTTSRFSEN GVWYPQKLNH GVSLRLQNTI QTESPIKFAV VHELGKQVIC 451 LLENGALQAW ECPTNRKEDS EQKQSYLRVE TRLKEEKKIH PIVMLNTPEP 501 KHSHERHFTA LIFSDGSIKA FEVSLTRGIF EVKSDSLDID GDDIYKISII 551 DPVHQTFVSN RPLISLITKK GLTRTYKAIV NYNDRHVQWI KACEINTGIM 601 NCTCIRGSST GKLCIVNSTG KVMSLWDLNR GVLEYEETFH NPIEDIDWTS 651 TEYGQSIVSI GFTGYALLYT QLRYDYTNNT PSYLPIEKID ITAHTAHNIG 701 DSVWMKNGTF VVASGNQFYI KDKSLDLTDP FTYQSIGSRK ILSNDILHLS 751 SVLNGPLPVY HPQFLIQAIY ANKLQLVKEL LLRLFLALRK LDFESQDVSN 801 LDSNLGMDPL KYFIAKDRDY PVESFPDPYP CFNKTVSLAL TEQLTKTTLP 851 YLTRHQQITL ITVIEAVDEV TKNENIVDYN GVRFLLGVKL FLSHKNIQKS 901 ILMRDVSWAL HSDNKEILLS SIDRHITSWN RAREYRIAYW IKEQDLVKKF 951 EDIAKYEFSK DDKRDPSRCA IFYLALKKKQ ILLSLWKMAI GHPEQQKMVR 1001 FISNDFTVPR WRTAALKNAF VLLSKHRYMD AAVFFLLTDS LKDCVNVLCK 1051 QVHDMDLAIG VCRVYEGDNG PVLGELLTAQ MLPETIKEND RWKASFIYWK 1101 LRKQEVAIKA LLTAPIDLEN NSSIVDKEVC VNRSFLVEDP ALLYLYNHLR 1151 NRNLKYFIGS LNVEAKIECT LILRVTDILC RMGCNYLAVS LVKNWKFIER 1201 NSIPVQKLLK SPTKDRAYSA IGAMASEPIS TARMRPSLFD KFGSPSASDI 1251 ESPNPKLPNS LLDDFLQPPP NSTSSNSLAQ SSSSAPRSIL DEFVSPSYSQ 1301 HKENLTPKAP NDSVGETDNS ENRKDKLSKD ILDDLSSQKP QKPKKSAITK 1351 NLLDDFV*

Amino Acid Sequence (or in FASTA format)

       1  MSLNFLPGRP NATPQTACQA TWQNHTIFAY CSGNNLIILT NKFTRLQTIY
      51  TQSDCTAVDI NSQNGFIALS FHNRVLIYKP IHQIMQNPKW TQCCQLFHDD
     101  TPVNCLRWSS DNELAIGSDF LSFWKIKDNF GVYQPILQWN QKQPKPVYNV
     151  IISQDSQLIV SIGKYDCNAK LWKRVSIVGE QAIFNLTMLP HPKPITAMRW
     201  KKEPDQVSKN NTASHALYTL CEDKVLRIWS CFEMEKNHTV QIWGEVPLSP
     251  TQKFCVIIDN WIIRQTLSVK DSEIFDISDS DIVILGSMTG EMEVLALNNL
     301  SQDPPKPMTK KTISHKKVKK ATMLNDTRYL YLPEIQPYDN VKGKLSFLVH
     351  DLQGVIRHLL IDILQLINNK TEDLSAALEH KFTGHNKSVQ KLVRSSDGEA
     401  LLTTSRFSEN GVWYPQKLNH GVSLRLQNTI QTESPIKFAV VHELGKQVIC
     451  LLENGALQAW ECPTNRKEDS EQKQSYLRVE TRLKEEKKIH PIVMLNTPEP
     501  KHSHERHFTA LIFSDGSIKA FEVSLTRGIF EVKSDSLDID GDDIYKISII
     551  DPVHQTFVSN RPLISLITKK GLTRTYKAIV NYNDRHVQWI KACEINTGIM
     601  NCTCIRGSST GKLCIVNSTG KVMSLWDLNR GVLEYEETFH NPIEDIDWTS
     651  TEYGQSIVSI GFTGYALLYT QLRYDYTNNT PSYLPIEKID ITAHTAHNIG
     701  DSVWMKNGTF VVASGNQFYI KDKSLDLTDP FTYQSIGSRK ILSNDILHLS
     751  SVLNGPLPVY HPQFLIQAIY ANKLQLVKEL LLRLFLALRK LDFESQDVSN
     801  LDSNLGMDPL KYFIAKDRDY PVESFPDPYP CFNKTVSLAL TEQLTKTTLP
     851  YLTRHQQITL ITVIEAVDEV TKNENIVDYN GVRFLLGVKL FLSHKNIQKS
     901  ILMRDVSWAL HSDNKEILLS SIDRHITSWN RAREYRIAYW IKEQDLVKKF
     951  EDIAKYEFSK DDKRDPSRCA IFYLALKKKQ ILLSLWKMAI GHPEQQKMVR
    1001  FISNDFTVPR WRTAALKNAF VLLSKHRYMD AAVFFLLTDS LKDCVNVLCK
    1051  QVHDMDLAIG VCRVYEGDNG PVLGELLTAQ MLPETIKEND RWKASFIYWK
    1101  LRKQEVAIKA LLTAPIDLEN NSSIVDKEVC VNRSFLVEDP ALLYLYNHLR
    1151  NRNLKYFIGS LNVEAKIECT LILRVTDILC RMGCNYLAVS LVKNWKFIER
    1201  NSIPVQKLLK SPTKDRAYSA IGAMASEPIS TARMRPSLFD KFGSPSASDI
    1251  ESPNPKLPNS LLDDFLQPPP NSTSSNSLAQ SSSSAPRSIL DEFVSPSYSQ
    1301  HKENLTPKAP NDSVGETDNS ENRKDKLSKD ILDDLSSQKP QKPKKSAITK
    1351  NLLDDFV*

external links for Rav1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Rav1p

1)	Seol JH, et al. (2001) Skp1 forms multiple protein complexes, including RAVE, a regulator of V-ATPase assembly. Nat Cell Biol 3(4):384-91
2)	Redding K, et al. (1996) Allele-specific suppression of a defective trans-Golgi network (TGN) localization signal in Kex2p identifies three genes involved in localization of TGN transmembrane proteins. Mol Cell Biol 16(11):6208-17
3)	Sipos G, et al. (2004) Soi3p/Rav1p functions at the early endosome to regulate endocytic trafficking to the vacuole and localization of trans-Golgi network transmembrane proteins. Mol Biol Cell 15(7):3196-209
4)	Ubersax JA, et al. (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
5)	Smardon AM, et al. (2002) The RAVE complex is essential for stable assembly of the yeast V-ATPase. J Biol Chem 277(16):13831-9
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41