GPI14/YJR013W Protein Information

Standard Name	Gpi14p 1
Systematic Name	Yjr013wp
Alias	Pmh1p 2
ORF Classification	Verified
Description	Glycosylphosphatidylinositol-alpha 1,4 mannosyltransferase I, involved in GPI anchor biosynthesis, requires Pbn1p for function; homolog of mammalian PIG-M (3, 4)
Name Description	GlycosylPhosphatidylInositol anchor biosynthesis 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	403
Molecular Weight (Da)	47,166
Isoelectric Point (pI)	9.86

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Gpi14p (InterPro)
Transmembrane Domains	There are 8 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 2 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000034F5CF \| P47088 MIPS: YJR013W NCBI: 1015644 \| 110825723 \| 1353052 \| 30267780 \| 42742257 \| 45269711 \| 854586 \| NP_012547.2 GenBank/EMBL/DDBJ: DAA08805.1 \| AY260895 \| AY557910 \| X87611 \| Z49513
External Classifications	EC: 2.4.1.- [Hexosyltransferases]

Amino Acid Sequence (or in FASTA format)
1 MTGEEWGLTV LSFLVRVGFF LFGIYQDANF KVRYTDIDYF VFHDAAKYVY 51 EGKSPYARDT YRYTPLLSWL LVPNHYFGWF HLGKVIFVIF DLVTGLIIMK 101 LLNQAISRKR ALILESIWLL NPMVITISTR GNAESVLCCL IMFTLFFLQK 151 SRYTLAGILY GLSIHFKIYP IIYCIPIAIF IYYNKRNQGP RTQLTSLLNI 201 GLSTLTTLLG CGWAMYKIYG YEFLDQAYLY HLYRTDHRHN FSVWNMLLYL 251 DSANKENGES NLSRYAFVPQ LLLVLVTGCL EWWNPTFDNL LRVLFVQTFA 301 FVTYNKVCTS QYFVWYLIFL PFYLSRTHIG WKKGLLMATL WVGTQGIWLS 351 QGYYLEFEGK NVFYPGLFIA SVLFFVTNVW LLGQFITDIK IPTQPTVSNK 401 KNN*

Amino Acid Sequence (or in FASTA format)

       1  MTGEEWGLTV LSFLVRVGFF LFGIYQDANF KVRYTDIDYF VFHDAAKYVY
      51  EGKSPYARDT YRYTPLLSWL LVPNHYFGWF HLGKVIFVIF DLVTGLIIMK
     101  LLNQAISRKR ALILESIWLL NPMVITISTR GNAESVLCCL IMFTLFFLQK
     151  SRYTLAGILY GLSIHFKIYP IIYCIPIAIF IYYNKRNQGP RTQLTSLLNI
     201  GLSTLTTLLG CGWAMYKIYG YEFLDQAYLY HLYRTDHRHN FSVWNMLLYL
     251  DSANKENGES NLSRYAFVPQ LLLVLVTGCL EWWNPTFDNL LRVLFVQTFA
     301  FVTYNKVCTS QYFVWYLIFL PFYLSRTHIG WKKGLLMATL WVGTQGIWLS
     351  QGYYLEFEGK NVFYPGLFIA SVLFFVTNVW LLGQFITDIK IPTQPTVSNK
     401  KNN*

external links for Gpi14p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome
YGOB	GeneMANIA
YOGY	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Gpi14p

1)	Kinoshita T and Inoue N (2000) Dissecting and manipulating the pathway for glycosylphos-phatidylinositol-anchor biosynthesis. Curr Opin Chem Biol 4(6):632-8
2)	Keranen, S (2003) personal communication
3)	Maeda Y, et al. (2001) PIG-M transfers the first mannose to glycosylphosphatidylinositol on the lumenal side of the ER. EMBO J 20(1-2):250-61
4)	Ashida H, et al. (2005) Mammalian PIG-X and yeast Pbn1p are the essential components of glycosylphosphatidylinositol-mannosyltransferase I. Mol Biol Cell 16(3):1439-48