MET3/YJR010W Protein Information

Standard Name	Met3p 1
Systematic Name	Yjr010wp
ORF Classification	Verified
Description	ATP sulfurylase, catalyzes the primary step of intracellular sulfate activation, essential for assimilatory reduction of sulfate to sulfide, involved in methionine metabolism (2, 3)
Name Description	METhionine requiring 1

Experimental Data
Molecules/cell	1510 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	511
Molecular Weight (Da)	57,724
Isoelectric Point (pI)	5.67

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Met3p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 8 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000168367 \| P08536 MIPS: YJR010W NCBI: 1015638 \| 127026 \| 14488754 \| 14488755 \| 14488756 \| 14488757 \| 14488758 \| 17942558 \| 17942559 \| 17942560 \| 17942561 \| 17942562 \| 33357107 \| 33357108 \| 33357109 \| 3928 \| 3937 \| 39654828 \| 398364775 \| 51830408 \| 854583 \| 88984437 \| NP_012543.3 GenBank/EMBL/DDBJ: DAA08801.1 \| AY723835 \| X06413 \| X60157 \| X87611 \| Z49510
External Classifications	EC: 2.7.7.4 [Sulfate adenylyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MPAPHGGILQ DLIARDALKK NELLSEAQSS DILVWNLTPR QLCDIELILN 51 GGFSPLTGFL NENDYSSVVT DSRLADGTLW TIPITLDVDE AFANQIKPDT 101 RIALFQDDEI PIAILTVQDV YKPNKTIEAE KVFRGDPEHP AISYLFNVAG 151 DYYVGGSLEA IQLPQHYDYP GLRKTPAQLR LEFQSRQWDR VVAFQTRNPM 201 HRAHRELTVR AAREANAKVL IHPVVGLTKP GDIDHHTRVR VYQEIIKRYP 251 NGIAFLSLLP LAMRMSGDRE AVWHAIIRKN YGASHFIVGR DHAGPGKNSK 301 GVDFYGPYDA QELVESYKHE LDIEVVPFRM VTYLPDEDRY APIDQIDTTK 351 TRTLNISGTE LRRRLRVGGE IPEWFSYPEV VKILRESNPP RPKQGFSIVL 401 GNSLTVSREQ LSIALLSTFL QFGGGRYYKI FEHNNKTELL SLIQDFIGSG 451 SGLIIPNQWE DDKDSVVGKQ NVYLLDTSSS ADIQLESADE PISHIVQKVV 501 LFLEDNGFFV F*

Amino Acid Sequence (or in FASTA format)

       1  MPAPHGGILQ DLIARDALKK NELLSEAQSS DILVWNLTPR QLCDIELILN
      51  GGFSPLTGFL NENDYSSVVT DSRLADGTLW TIPITLDVDE AFANQIKPDT
     101  RIALFQDDEI PIAILTVQDV YKPNKTIEAE KVFRGDPEHP AISYLFNVAG
     151  DYYVGGSLEA IQLPQHYDYP GLRKTPAQLR LEFQSRQWDR VVAFQTRNPM
     201  HRAHRELTVR AAREANAKVL IHPVVGLTKP GDIDHHTRVR VYQEIIKRYP
     251  NGIAFLSLLP LAMRMSGDRE AVWHAIIRKN YGASHFIVGR DHAGPGKNSK
     301  GVDFYGPYDA QELVESYKHE LDIEVVPFRM VTYLPDEDRY APIDQIDTTK
     351  TRTLNISGTE LRRRLRVGGE IPEWFSYPEV VKILRESNPP RPKQGFSIVL
     401  GNSLTVSREQ LSIALLSTFL QFGGGRYYKI FEHNNKTELL SLIQDFIGSG
     451  SGLIIPNQWE DDKDSVVGKQ NVYLLDTSSS ADIQLESADE PISHIVQKVV
     501  LFLEDNGFFV F*

external links for Met3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Met3p

1)	Masselot M and De Robichon-Szulmajster H (1975) Methionine biosynthesis in Saccharomyces cerevisiae. I. Genetical analysis of auxotrophic mutants. Mol Gen Genet 139(2):121-32
2)	Cherest H, et al. (1985) Transcriptional regulation of the MET3 gene of Saccharomyces cerevisiae. Gene 34(2-3):269-81
3)	Ullrich TC, et al. (2001) Crystal structure of ATP sulfurylase from Saccharomyces cerevisiae, a key enzyme in sulfate activation. EMBO J 20(3):316-29
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41