MNN5/YJL186W Protein Information

Standard Name	Mnn5p
Systematic Name	Yjl186wp
ORF Classification	Verified
Description	Alpha-1,2-mannosyltransferase, responsible for addition of the second alpha-1,2-linked mannose of the branches on the mannan backbone of oligosaccharides, localizes to an early Golgi compartment (1)
Name Description	MaNNosyltransferase

Experimental Data
Molecules/cell	11400 2

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	586
Molecular Weight (Da)	67,204
Isoelectric Point (pI)	6.87

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mnn5p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 6 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0001F7BD4E \| P46982 MIPS: YJL186W NCBI: 1008393 \| 1353043 \| 330443597 \| 347595670 \| NP_012349.2 GenBank/EMBL/DDBJ: DAA08620.2 \| Z49461

Amino Acid Sequence (or in FASTA format)
1 MLIRLKKRKI LQVIVSAVVL ILFFCSVHND VSSSWLYGKK LRLPVLTRSN 51 LKNNFYTTLV QAIVENKPAD SSPDLSKLHG AEGCSFANNV AAHDSGHDSD 101 LSYESLSKCY NLNKTVQESL REVHSKFTDT LSGKLNFSIP QREALFSGSE 151 GIVTIGGGKY SVLAYTMIKK LRDTGTTLPI EVIIPPQDEG EDDFCKNWLP 201 KFNGKCIYFS DIVPSKPLSD LKLTHFQLKV FGLIISSFKR IIFLDADNYA 251 VKNLDLAFNT TSFNDTGLIL WPDFWRRVTP PAFYNIIGSS INIGKRVRFV 301 SDDISPVSRY DPFVSNSNDY TPKERQEHFL KHVPLHDLDG TMPDLSSESG 351 QMVIDKIRHF NTLLLALYYN VYGPTWYYKM ISQGTAGEGD KDTFVAAAHA 401 LNMPYYQVRT NFEFDGFFYQ KDDYKGLALL QHDFEQDYKQ YQKAQQKVKA 451 NIEEFSKLDP DYTLDNGFLK TLMVNDDGSD LDIMFIHASF YKADPWTLYH 501 ENRFIGPNGE QVRGFRKPHR YGMDFELFLF NDMRGSFCTT PKSQVIKFKY 551 FTDKVNTPEW DAMCEYLTNH VNYLESTHKE AMGEKN*

Amino Acid Sequence (or in FASTA format)

       1  MLIRLKKRKI LQVIVSAVVL ILFFCSVHND VSSSWLYGKK LRLPVLTRSN
      51  LKNNFYTTLV QAIVENKPAD SSPDLSKLHG AEGCSFANNV AAHDSGHDSD
     101  LSYESLSKCY NLNKTVQESL REVHSKFTDT LSGKLNFSIP QREALFSGSE
     151  GIVTIGGGKY SVLAYTMIKK LRDTGTTLPI EVIIPPQDEG EDDFCKNWLP
     201  KFNGKCIYFS DIVPSKPLSD LKLTHFQLKV FGLIISSFKR IIFLDADNYA
     251  VKNLDLAFNT TSFNDTGLIL WPDFWRRVTP PAFYNIIGSS INIGKRVRFV
     301  SDDISPVSRY DPFVSNSNDY TPKERQEHFL KHVPLHDLDG TMPDLSSESG
     351  QMVIDKIRHF NTLLLALYYN VYGPTWYYKM ISQGTAGEGD KDTFVAAAHA
     401  LNMPYYQVRT NFEFDGFFYQ KDDYKGLALL QHDFEQDYKQ YQKAQQKVKA
     451  NIEEFSKLDP DYTLDNGFLK TLMVNDDGSD LDIMFIHASF YKADPWTLYH
     501  ENRFIGPNGE QVRGFRKPHR YGMDFELFLF NDMRGSFCTT PKSQVIKFKY
     551  FTDKVNTPEW DAMCEYLTNH VNYLESTHKE AMGEKN*

external links for Mnn5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Aspergillus (AspGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mnn5p

1)	Rayner JC and Munro S (1998) Identification of the MNN2 and MNN5 mannosyltransferases required for forming and extending the mannose branches of the outer chain mannans of Saccharomyces cerevisiae. J Biol Chem 273(41):26836-43
2)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41