SET2/YJL168C Protein Information

Standard Name	Set2p 1
Systematic Name	Yjl168cp
Alias	Ezl1p
ORF Classification	Verified
Description	Histone methyltransferase with a role in transcriptional elongation; methylates H3 lysine 36, which suppresses incorporation of acetylated histones and signals for the deacetylation of these histones within transcribed genes; associates with the C-terminal domain of Rpo21p; histone methylation activity is regulated by phosphorylation status of Rpo21p (2, 3, 4, 5, 6)
Name Description	SET domain-containing 7
Gene Product	KMT3 8

Experimental Data
Molecules/cell	217 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	733
Molecular Weight (Da)	84,461
Isoelectric Point (pI)	8.67

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Set2p (InterPro)
Physical Interactions	There are 139 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0001F7BD4B \| P46995 MIPS: YJL168C NCBI: 1008361 \| 1353031 \| 330443605 \| 347595709 \| 83754681 \| 8569662 \| NP_012367.2 GenBank/EMBL/DDBJ: DAA08635.2 \| Z49444
External Classifications	EC: 2.1.1.43 [Histone-lysine N-methyltransferase]

Amino Acid Sequence (or in FASTA format)
1 MSKNQSVSAS EDEKEILNNN AEGHKPQRLF DQEPDLTEEA LTKFENLDDC 51 IYANKRIGTF KNNDFMECDC YEEFSDGVNH ACDEDSDCIN RLTLIECVND 101 LCSSCGNDCQ NQRFQKKQYA PIAIFKTKHK GYGVRAEQDI EANQFIYEYK 151 GEVIEEMEFR DRLIDYDQRH FKHFYFMMLQ NGEFIDATIK GSLARFCNHS 201 CSPNAYVNKW VVKDKLRMGI FAQRKILKGE EITFDYNVDR YGAQAQKCYC 251 EEPNCIGFLG GKTQTDAASL LPQNIADALG VTVSMEKKWL KLKKLSGEPI 301 IKNENENINI EFLQSLEVQP IDSPVDVTKI MSVLLQQDNK IIASKLLKRL 351 FTIDDDSLRH QAIKLHGYTC FSKMLKLFIT EQPQVDGKGN ETEEDDIKFI 401 KGILDFLLEL PKTTRNGIES SQIDNVVKTL PAKFPFLKPN CDELLEKWSK 451 FETYKRITKK DINVAASKMI DLRRVRLPPG WEIIHENGRP LYYNAEQKTK 501 LHYPPSGSSK VFSSRSNTQV NSPSSSGIPK TPGALDSKKH KLSDEEYERK 551 KQKRLEYERI ALERAKQEEL ESLKQKLKLE NERKSVLEDI IAEANKQKEL 601 QKEEAKKLVE AKEAKRLKRK TVSQSQRLEH NWNKFFASFV PNLIKKNPQS 651 KQFDHENIKQ CAKDIVKILT TKELKKDSSR APPDDLTKGK RHKVKEFINS 701 YMDKIILKKK QKKALALSSA STRMSSPPPS TSS*

Amino Acid Sequence (or in FASTA format)

       1  MSKNQSVSAS EDEKEILNNN AEGHKPQRLF DQEPDLTEEA LTKFENLDDC
      51  IYANKRIGTF KNNDFMECDC YEEFSDGVNH ACDEDSDCIN RLTLIECVND
     101  LCSSCGNDCQ NQRFQKKQYA PIAIFKTKHK GYGVRAEQDI EANQFIYEYK
     151  GEVIEEMEFR DRLIDYDQRH FKHFYFMMLQ NGEFIDATIK GSLARFCNHS
     201  CSPNAYVNKW VVKDKLRMGI FAQRKILKGE EITFDYNVDR YGAQAQKCYC
     251  EEPNCIGFLG GKTQTDAASL LPQNIADALG VTVSMEKKWL KLKKLSGEPI
     301  IKNENENINI EFLQSLEVQP IDSPVDVTKI MSVLLQQDNK IIASKLLKRL
     351  FTIDDDSLRH QAIKLHGYTC FSKMLKLFIT EQPQVDGKGN ETEEDDIKFI
     401  KGILDFLLEL PKTTRNGIES SQIDNVVKTL PAKFPFLKPN CDELLEKWSK
     451  FETYKRITKK DINVAASKMI DLRRVRLPPG WEIIHENGRP LYYNAEQKTK
     501  LHYPPSGSSK VFSSRSNTQV NSPSSSGIPK TPGALDSKKH KLSDEEYERK
     551  KQKRLEYERI ALERAKQEEL ESLKQKLKLE NERKSVLEDI IAEANKQKEL
     601  QKEEAKKLVE AKEAKRLKRK TVSQSQRLEH NWNKFFASFV PNLIKKNPQS
     651  KQFDHENIKQ CAKDIVKILT TKELKKDSSR APPDDLTKGK RHKVKEFINS
     701  YMDKIILKKK QKKALALSSA STRMSSPPPS TSS*

external links for Set2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Set2p

1)	Hesman, T. and M. Johnston (1997) SET2p contains both SET and CXC domains and regulates GAL4 expression. Unpublished
2)	Strahl BD, et al. (2002) Set2 is a nucleosomal histone H3-selective methyltransferase that mediates transcriptional repression. Mol Cell Biol 22(5):1298-306
3)	Xiao T, et al. (2003) Phosphorylation of RNA polymerase II CTD regulates H3 methylation in yeast. Genes Dev 17(5):654-63
4)	Schaft D, et al. (2003) The histone 3 lysine 36 methyltransferase, SET2, is involved in transcriptional elongation. Nucleic Acids Res 31(10):2475-82
5)	Li B, et al. (2003) The Set2 histone methyltransferase functions through the phosphorylated carboxyl-terminal domain of RNA polymerase II. J Biol Chem 278(11):8897-903
6)	Venkatesh S, et al. (2012) Set2 methylation of histone H3 lysine?36 suppresses histone exchange on transcribed genes. Nature 489(7416):452-5
7)	Nislow C, et al. (1997) SET1, a yeast member of the trithorax family, functions in transcriptional silencing and diverse cellular processes. Mol Biol Cell 8(12):2421-36
8)	Allis CD, et al. (2007) New nomenclature for chromatin-modifying enzymes. Cell 131(4):633-6
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41