TPK1/YJL164C Protein Information Help

Standard Name Tpk1p 1
Systematic Name Yjl164cp
Alias Pka1p , Sra3p
ORF Classification Verified
Description cAMP-dependent protein kinase catalytic subunit; promotes vegetative growth in response to nutrients via the Ras-cAMP signaling pathway; inhibited by regulatory subunit Bcy1p in the absence of cAMP; regulates Whi3p; partially redundant with Tpk2p and Tpk3p; phosphorylates pre-Tom40p, which impairs its import into mitochondria under non-respiratory conditions; TPK1 has a paralog, TPK3, that arose from the whole genome duplication (2, 3, 4, 5, 6, 7)
Name Description Takashi's Protein Kinase 8
Experimental Data
Molecules/cell 4320 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 397
Molecular Weight (Da) 46,076
Isoelectric Point (pI) 5.14

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tpk1p (InterPro)
Physical Interactions There are 361 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012DC18 | P06244
MIPS: YJL164C
NCBI: 1008352 | 125208 | 14719777 | 172175 | 172691 | 173009 | 330443607 | 347595790 | 62467585 | NP_012371.2 | NM_001181597.2
GenBank/EMBL/DDBJ: DAA08639.2 | AY899257 | J02665 | M17072 | M17224 | Z49439
External Classifications EC: 2.7.11.11 [cAMP-dependent protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSTEEQNGGG QKSLDDRQGE ESQKGETSER ETTATESGNE SKSVEKEGGE
      51  TQEKPKQPHV TYYNEEQYKQ FIAQARVTSG KYSLQDFQIL RTLGTGSFGR
     101  VHLIRSRHNG RYYAMKVLKK EIVVRLKQVE HTNDERLMLS IVTHPFIIRM
     151  WGTFQDAQQI FMIMDYIEGG ELFSLLRKSQ RFPNPVAKFY AAEVCLALEY
     201  LHSKDIIYRD LKPENILLDK NGHIKITDFG FAKYVPDVTY TLCGTPDYIA
     251  PEVVSTKPYN KSIDWWSFGI LIYEMLAGYT PFYDSNTMKT YEKILNAELR
     301  FPPFFNEDVK DLLSRLITRD LSQRLGNLQN GTEDVKNHPW FKEVVWEKLL
     351  SRNIETPYEP PIQQGQGDTS QFDKYPEEDI NYGVQGEDPY ADLFRDF*

external links for Tpk1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Tpk1p
1) Cannon, J.F.  (1989) Personal Communication, Mortimer Map Edition 10
2) Toda T, et al.  (1987) Three different genes in S. cerevisiae encode the catalytic subunits of the cAMP-dependent protein kinase. Cell 50(2):277-87
3) Ordiz I, et al.  (1996) Glucose-induced inactivation of isocitrate lyase in Saccharomyces cerevisiae is mediated by the cAMP-dependent protein kinase catalytic subunits Tpk1 and Tpk2. FEBS Lett 385(1-2):43-6
4) Toda T, et al.  (1987) Cloning and characterization of BCY1, a locus encoding a regulatory subunit of the cyclic AMP-dependent protein kinase in Saccharomyces cerevisiae. Mol Cell Biol 7(4):1371-7
5) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
6) Rao S, et al.  (2012) Biogenesis of the preprotein translocase of the outer mitochondrial membrane: protein kinase A phosphorylates the precursor of Tom40 and impairs its import. Mol Biol Cell 23(9):1618-27
7) Mizunuma M, et al.  (2013) Ras/cAMP-dependent protein kinase (PKA) regulates multiple aspects of cellular events by phosphorylating the Whi3 cell cycle regulator in budding yeast. J Biol Chem 288(15):10558-66
8) Thorner J  (2005) personal communication
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41