HSP150/YJL159W Protein Information

Standard Name	Hsp150p
Systematic Name	Yjl159wp
Alias	Ccw7p 1 , Ore1p 2 , Pir2p 3
ORF Classification	Verified
Description	O-mannosylated heat shock protein; secreted and covalently attached to the cell wall via beta-1,3-glucan and disulfide bridges; required for cell wall stability; induced by heat shock, oxidative stress, and nitrogen limitation; HSP150 has a paralog, PIR3, that arose from the whole genome duplication (10, 3, 4, 5, 6, 7, 8, 9)
Name Description	Heat Shock Protein 8

Experimental Data
Molecules/cell	14600 11

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	413
Molecular Weight (Da)	41,059
Isoelectric Point (pI)	4.86

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Hsp150p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 10 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004AD16 \| P32478 MIPS: YJL159W NCBI: 1015583 \| 116006493 \| 164710430 \| 164710432 \| 164710434 \| 218459 \| 256081 \| 30267752 \| 34979819 \| 473374 \| 73915352 \| NP_012376.3 \| NM_001181592.1 GenBank/EMBL/DDBJ: DAA08644.1 \| AY260881 \| AY321583 \| D13741 \| EU220722 \| EU220723 \| EU220724 \| M88698 \| S45000 \| Z49434

Amino Acid Sequence (or in FASTA format)
1 MQYKKTLVAS ALAATTLAAY APSEPWSTLT PTATYSGGVT DYASTFGIAV 51 QPISTTSSAS SAATTASSKA KRAASQIGDG QVQAATTTAS VSTKSTAAAV 101 SQIGDGQIQA TTKTTAAAVS QIGDGQIQAT TKTTSAKTTA AAVSQISDGQ 151 IQATTTTLAP KSTAAAVSQI GDGQVQATTT TLAPKSTAAA VSQIGDGQVQ 201 ATTKTTAAAV SQIGDGQVQA TTKTTAAAVS QIGDGQVQAT TKTTAAAVSQ 251 IGDGQVQATT KTTAAAVSQI TDGQVQATTK TTQAASQVSD GQVQATTATS 301 ASAAATSTDP VDAVSCKTSG TLEMNLKGGI LTDGKGRIGS IVANRQFQFD 351 GPPPQAGAIY AAGWSITPDG NLAIGDNDVF YQCLSGTFYN LYDEHIGSQC 401 TPVHLEAIDL IDC*

Amino Acid Sequence (or in FASTA format)

       1  MQYKKTLVAS ALAATTLAAY APSEPWSTLT PTATYSGGVT DYASTFGIAV
      51  QPISTTSSAS SAATTASSKA KRAASQIGDG QVQAATTTAS VSTKSTAAAV
     101  SQIGDGQIQA TTKTTAAAVS QIGDGQIQAT TKTTSAKTTA AAVSQISDGQ
     151  IQATTTTLAP KSTAAAVSQI GDGQVQATTT TLAPKSTAAA VSQIGDGQVQ
     201  ATTKTTAAAV SQIGDGQVQA TTKTTAAAVS QIGDGQVQAT TKTTAAAVSQ
     251  IGDGQVQATT KTTAAAVSQI TDGQVQATTK TTQAASQVSD GQVQATTATS
     301  ASAAATSTDP VDAVSCKTSG TLEMNLKGGI LTDGKGRIGS IVANRQFQFD
     351  GPPPQAGAIY AAGWSITPDG NLAIGDNDVF YQCLSGTFYN LYDEHIGSQC
     401  TPVHLEAIDL IDC*

external links for Hsp150p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
YGOB	BioPIXIE	MIPS	YeastGFP
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Hsp150p

1)	Mrsa V, et al. (1997) Specific labelling of cell wall proteins by biotinylation. Identification of four covalently linked O-mannosylated proteins of Saccharomyces cerevisiae. Yeast 13(12):1145-54
2)	Yun DJ, et al. (1997) Stress proteins on the yeast cell surface determine resistance to osmotin, a plant antifungal protein. Proc Natl Acad Sci U S A 94(13):7082-7
3)	Toh-e A, et al. (1993) Three yeast genes, PIR1, PIR2 and PIR3, containing internal tandem repeats, are related to each other, and PIR1 and PIR2 are required for tolerance to heat shock. Yeast 9(5):481-94
4)	Mrsa V and Tanner W (1999) Role of NaOH-extractable cell wall proteins Ccw5p, Ccw6p, Ccw7p and Ccw8p (members of the Pir protein family) in stability of the Saccharomyces cerevisiae cell wall. Yeast 15(10A):813-20
5)	Ezaki B, et al. (1998) Protective roles of two aluminum (Al)-induced genes, HSP150 and SED1 of Saccharomyces cerevisiae, in Al and oxidative stresses. FEMS Microbiol Lett 159(1):99-105
6)	Russo P, et al. (1993) Dual regulation by heat and nutrient stress of the yeast HSP150 gene encoding a secretory glycoprotein. Mol Gen Genet 239(1-2):273-80
7)	Kapteyn JC, et al. (1999) The contribution of the O-glycosylated protein Pir2p/Hsp150 to the construction of the yeast cell wall in wild-type cells and beta 1,6-glucan-deficient mutants. Mol Microbiol 31(6):1835-44
8)	Russo P, et al. (1992) A heat shock gene from Saccharomyces cerevisiae encoding a secretory glycoprotein. Proc Natl Acad Sci U S A 89(9):3671-5
9)	Moukadiri I and Zueco J (2001) Evidence for the attachment of Hsp150/Pir2 to the cell wall of Saccharomyces cerevisiae through disulfide bridges. FEMS Yeast Res 1(3):241-5
10)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
11)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41