YAK1/YJL141C Protein Information

Standard Name	Yak1p 1
Systematic Name	Yjl141cp
ORF Classification	Verified
Description	Serine-threonine protein kinase; component of a glucose-sensing system that inhibits growth in response to glucose availability; upon nutrient deprivation Yak1p phosphorylates Pop2p to regulate mRNA deadenylation, the co-repressor Crf1p to inhibit transcription of ribosomal genes, and the stress-responsive transcription factors Hsf1p and Msn2p; nuclear localization negatively regulated by the Ras/PKA signaling pathway in the presence of glucose (2, 3, 4, 5, 6, 7)
Name Description	Yet Another Kinase 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	807
Molecular Weight (Da)	91,245
Isoelectric Point (pI)	8.43

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yak1p (InterPro)
Physical Interactions	There are 80 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013A0FE \| P14680 MIPS: YJL141C NCBI: 1015551 \| 140201 \| 4796 \| 6322320 \| 854556 \| NP_012394.1 GenBank/EMBL/DDBJ: DAA08659.1 \| X16056 \| X87371 \| Z49417
External Classifications	EC: 2.7.12.1 [Dual-specificity kinase]

Amino Acid Sequence (or in FASTA format)
1 MNSSNNNDSS SSNSNMNNSL SPTLVTHSDA SMGSGRASPD NSHMGRGIWN 51 PSYVNQGSQR SPQQQHQNHH QQQQQQQQQQ QQNSQFCFVN PWNEEKVTNS 101 QQNLVYPPQY DDLNSNESLD AYRRRKSSLV VPPARAPAPN PFQYDSYPAY 151 TSSNTSLAGN SSGQYPSGYQ QQQQQVYQQG AIHPSQFGSR FVPSLYDRQD 201 FQRRQSLAAT NYSSNFSSLN SNTNQGTNSI PVMSPYRRLS AYPPSTSPPL 251 QPPFKQLRRD EVQGQKLSIP QMQLCNSKND LQPVLNATPK FRRASLNSKT 301 ISPLVSVTKS LITTYSLCSP EFTYQTSKNP KRVLTKPSEG KCNNGFDNIN 351 SDYILYVNDV LGVEQNRKYL VLDILGQGTF GQVVKCQNLL TKEILAVKVV 401 KSRTEYLTQS ITEAKILELL NQKIDPTNKH HFLRMYDSFV HKNHLCLVFE 451 LLSNNLYELL KQNKFHGLSI QLIRTFTTQI LDSLCVLKES KLIHCDLKPE 501 NILLCAPDKP ELKIIDFGSS CEEARTVYTY IQSRFYRAPE IILGIPYSTS 551 IDMWSLGCIV AELFLGIPIF PGASEYNQLT RIIDTLGYPP SWMIDMGKNS 601 GKFMKKLAPE ESSSSTQKHR MKTIEEFCRE YNIVEKPSKQ YFKWRKLPDI 651 IRNYRYPKSI QNSQELIDQE MQNRECLIHF LGGVLNLNPL ERWTPQQAML 701 HPFITKQEFT GEWFPPGSSL PGPSEKHDDA KGQQSEYGSA NDSSNNAGHN 751 YVYNPSSATG GADSVDIGAI SKRKENTSGD ISNNFAVTHS VQEGPTSAFN 801 KLHIVEE*

Amino Acid Sequence (or in FASTA format)

       1  MNSSNNNDSS SSNSNMNNSL SPTLVTHSDA SMGSGRASPD NSHMGRGIWN
      51  PSYVNQGSQR SPQQQHQNHH QQQQQQQQQQ QQNSQFCFVN PWNEEKVTNS
     101  QQNLVYPPQY DDLNSNESLD AYRRRKSSLV VPPARAPAPN PFQYDSYPAY
     151  TSSNTSLAGN SSGQYPSGYQ QQQQQVYQQG AIHPSQFGSR FVPSLYDRQD
     201  FQRRQSLAAT NYSSNFSSLN SNTNQGTNSI PVMSPYRRLS AYPPSTSPPL
     251  QPPFKQLRRD EVQGQKLSIP QMQLCNSKND LQPVLNATPK FRRASLNSKT
     301  ISPLVSVTKS LITTYSLCSP EFTYQTSKNP KRVLTKPSEG KCNNGFDNIN
     351  SDYILYVNDV LGVEQNRKYL VLDILGQGTF GQVVKCQNLL TKEILAVKVV
     401  KSRTEYLTQS ITEAKILELL NQKIDPTNKH HFLRMYDSFV HKNHLCLVFE
     451  LLSNNLYELL KQNKFHGLSI QLIRTFTTQI LDSLCVLKES KLIHCDLKPE
     501  NILLCAPDKP ELKIIDFGSS CEEARTVYTY IQSRFYRAPE IILGIPYSTS
     551  IDMWSLGCIV AELFLGIPIF PGASEYNQLT RIIDTLGYPP SWMIDMGKNS
     601  GKFMKKLAPE ESSSSTQKHR MKTIEEFCRE YNIVEKPSKQ YFKWRKLPDI
     651  IRNYRYPKSI QNSQELIDQE MQNRECLIHF LGGVLNLNPL ERWTPQQAML
     701  HPFITKQEFT GEWFPPGSSL PGPSEKHDDA KGQQSEYGSA NDSSNNAGHN
     751  YVYNPSSATG GADSVDIGAI SKRKENTSGD ISNNFAVTHS VQEGPTSAFN
     801  KLHIVEE*

external links for Yak1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Yak1p

1)	Garrett S and Broach J (1989) Loss of Ras activity in Saccharomyces cerevisiae is suppressed by disruptions of a new kinase gene, YAKI, whose product may act downstream of the cAMP-dependent protein kinase. Genes Dev 3(9):1336-48
2)	Garrett S, et al. (1991) The Saccharomyces cerevisiae YAK1 gene encodes a protein kinase that is induced by arrest early in the cell cycle. Mol Cell Biol 11(8):4045-52
3)	Kassis S, et al. (2000) Saccharomyces cerevisiae Yak1p protein kinase autophosphorylates on tyrosine residues and phosphorylates myelin basic protein on a C-terminal serine residue. Biochem J 348 Pt 2():263-72
4)	Moriya H, et al. (2001) Yak1p, a DYRK family kinase, translocates to the nucleus and phosphorylates yeast Pop2p in response to a glucose signal. Genes Dev 15(10):1217-28
5)	Martin DE, et al. (2004) TOR regulates ribosomal protein gene expression via PKA and the Forkhead transcription factor FHL1. Cell 119(7):969-79
6)	Lee P, et al. (2008) Yeast Yak1 kinase, a bridge between PKA and stress-responsive transcription factors, Hsf1 and Msn2/Msn4. Mol Microbiol 70(4):882-95
7)	Lee P, et al. (2011) Regulation of yeast Yak1 kinase by PKA and autophosphorylation-dependent 14-3-3 binding. Mol Microbiol 79(3):633-46