RPB4/YJL140W Protein Information Help

Standard Name Rpb4p 1, 2
Systematic Name Yjl140wp
Alias Ctf15p 3
ORF Classification Verified
Description RNA polymerase II subunit B32; forms two subunit dissociable complex with Rpb7p; involved in recruitment of 3'-end processing factors to transcribing RNA polymerase II complex and in export of mRNA to cytoplasm under stress conditions; also involved in translation initiation (10, 4, 5, 6, 7, 8, 9)
Name Description RNA Polymerase B 1
Gene Product B32 11
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 221
Molecular Weight (Da) 25,414
Isoelectric Point (pI) 4.66

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Rpb4p (InterPro)
Physical Interactions There are 160 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053193 | P20433
MIPS: YJL140W
NCBI: 1015548 | 110590857 | 133336 | 134104677 | 134104692 | 134104713 | 134104725 | 134104737 | 160877776 | 161172230 | 161172243 | 190613465 | 224983593 | 240104514 | 254839444 | 254839459 | 254839474 | 254839489 | 254839504 | 254839519 | 254839534 | 257471949 | 257471964 | 266618768 | 295648 | 30749740 | 325053966 | 325053981 | 326634541 | 34810556 | 357380839 | 359546293 | 359546311 | 359546328 | 359546345 | 359546362 | 359546379 | 359546395 | 359546412 | 374977882 | 374977897 | 380764982 | 407280318 | 414145449 | 414145462 | 45269603 | 459358297 | 4834 | 61679447 | 61679464 | 61679505 | 61679518 | 61679565 | 61679567 | 6322321 | 83754246 | 854557 | NP_012395.1
GenBank/EMBL/DDBJ: DAA08660.1 | AY557856 | M27253 | X58099 | X87371 | Z49415
Amino Acid Sequence (or in FASTA format)
       1  MNVSTSTFQT RRRRLKKVEE EENAATLQLG QEFQLKQINH QGEEEELIAL
      51  NLSEARLVIK EALVERRRAF KRSQKKHKKK HLKHENANDE TTAVEDEDDD
     101  LDEDDVNADD DDFMHSETRE KELESIDVLL EQTTGGNNKD LKNTMQYLTN
     151  FSRFRDQETV GAVIQLLKST GLHPFEVAQL GSLACDTADE AKTLIPSLNN
     201  KISDDELERI LKELSNLETL Y*                              

external links for Rpb4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Rpb4p
1) Nonet M, et al.  (1987) Eucaryotic RNA polymerase conditional mutant that rapidly ceases mRNA synthesis. Mol Cell Biol 7(5):1602-11
2) Woychik NA and Young RA  (1989) RNA polymerase II subunit RPB4 is essential for high- and low-temperature yeast cell growth. Mol Cell Biol 9(7):2854-9
3) Spencer F, et al.  (1990) Mitotic chromosome transmission fidelity mutants in Saccharomyces cerevisiae. Genetics 124(2):237-49
4) Archambault J and Friesen JD  (1993) Genetics of eukaryotic RNA polymerases I, II, and III. Microbiol Rev 57(3):703-24
5) Hampsey M  (1998) Molecular genetics of the RNA polymerase II general transcriptional machinery. Microbiol Mol Biol Rev 62(2):465-503
6) Cramer P, et al.  (2000) Architecture of RNA polymerase II and implications for the transcription mechanism. Science 288(5466):640-9
7) Choder M  (2004) Rpb4 and Rpb7: subunits of RNA polymerase II and beyond. Trends Biochem Sci 29(12):674-81
8) Sampath V and Sadhale P  (2005) Rpb4 and Rpb7: a sub-complex integral to multi-subunit RNA polymerases performs a multitude of functions. IUBMB Life 57(2):93-102
9) Runner VM, et al.  (2008) The Rpb4 subunit of RNA polymerase II contributes to cotranscriptional recruitment of 3' processing factors. Mol Cell Biol 28(6):1883-91
10) Harel-Sharvit L, et al.  (2010) RNA Polymerase II Subunits Link Transcription and mRNA Decay to Translation. Cell 143(4):552-63
11) Sentenac A  (1985) Eukaryotic RNA polymerases. CRC Crit Rev Biochem 18(1):31-90