PBS2/YJL128C Protein Information Help

Standard Name Pbs2p
Systematic Name Yjl128cp
Alias Hog4p , Sfs4p , Ssk4p 1, 2
ORF Classification Verified
Description MAP kinase kinase of the HOG signaling pathway; activated under severe osmotic stress; mitophagy-specific regulator; plays a role in regulating Ty1 transposition (3, 4, 5)
Name Description Polymyxin B Sensitivity 6
Experimental Data
Molecules/cell 2160 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 668
Molecular Weight (Da) 72,720
Isoelectric Point (pI) 9.44

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pbs2p (InterPro)
Physical Interactions There are 71 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI00001313B0 | P08018
MIPS: YJL128C
NCBI: 1008328 | 167013341 | 330443611 | 347595821 | 460913 | 517224 | 83305209 | NP_012407.2
GenBank/EMBL/DDBJ: DAA08673.2 | J02946 | U12237 | Z49403
External Classifications EC: 2.7.12.2 [Mitogen-activated protein kinase kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MEDKFANLSL HEKTGKSSIQ LNEQTGSDNG SAVKRTSSTS SHYNNINADL
      51  HARVKAFQEQ RALKRSASVG SNQSEQDKGS SQSPKHIQQI VNKPLPPLPV
     101  AGSSKVSQRM SSQVVQASSK STLKNVLDNQ ETQNITDVNI NIDTTKITAT
     151  TIGVNTGLPA TDITPSVSNT ASATHKAQLL NPNRRAPRRP LSTQHPTRPN
     201  VAPHKAPAII NTPKQSLSAR RGLKLPPGGM SLKMPTKTAQ QPQQFAPSPS
     251  NKKHIETLSN SKVVEGKRSN PGSLINGVQS TSTSSSTEGP HDTVGTTPRT
     301  GNSNNSSNSG SSGGGGLFAN FSKYVDIKSG SLNFAGKLSL SSKGIDFSNG
     351  SSSRITLDEL EFLDELGHGN YGNVSKVLHK PTNVIMATKE VRLELDEAKF
     401  RQILMELEVL HKCNSPYIVD FYGAFFIEGA VYMCMEYMDG GSLDKIYDES
     451  SEIGGIDEPQ LAFIANAVIH GLKELKEQHN IIHRDVKPTN ILCSANQGTV
     501  KLCDFGVSGN LVASLAKTNI GCQSYMAPER IKSLNPDRAT YTVQSDIWSL
     551  GLSILEMALG RYPYPPETYD NIFSQLSAIV DGPPPRLPSD KFSSDAQDFV
     601  SLCLQKIPER RPTYAALTEH PWLVKYRNQD VHMSEYITER LERRNKILRE
     651  RGENGLSKNV PALHMGGL*

external links for Pbs2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Pbs2p
1) Maeda T, et al.  (1994) A two-component system that regulates an osmosensing MAP kinase cascade in yeast. Nature 369(6477):242-5
2) Mao K, et al.  (2011) Two MAPK-signaling pathways are required for mitophagy in Saccharomyces cerevisiae. J Cell Biol 193(4):755-67
3) Van Wuytswinkel O, et al.  (2000) Response of Saccharomyces cerevisiae to severe osmotic stress: evidence for a novel activation mechanism of the HOG MAP kinase pathway. Mol Microbiol 37(2):382-97
4) Bansal PK, et al.  (2001) A PBS2 homologue from Debaryomyces hansenii shows a differential effect on calcofluor and polymyxin B sensitivity in Saccharomyces cerevisiae. Yeast 18(13):1207-16
5) Nyswaner KM, et al.  (2008) Chromatin-associated genes protect the yeast genome from ty1 insertional mutagenesis. Genetics 178(1):197-214
6) Boguslawski G and Polazzi JO  (1987) Complete nucleotide sequence of a gene conferring polymyxin B resistance on yeast: similarity of the predicted polypeptide to protein kinases. Proc Natl Acad Sci U S A 84(16):5848-52
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41