GCD14/YJL125C Protein Information Help

Standard Name Gcd14p 1
Systematic Name Yjl125cp
Alias Trm61p 2
ORF Classification Verified
Description Subunit of tRNA (1-methyladenosine) methyltransferase, with Gcd10p, required for the modification of the adenine at position 58 in tRNAs, especially tRNAi-Met; first identified as a negative regulator of GCN4 expression (3, 4, 5)
Name Description General Control Nonderepressible 6
Gene Product 1-methyladenosine tRNA methyltransferase subunit 3
Experimental Data
Molecules/cell 7220 7
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 383
Molecular Weight (Da) 43,919
Isoelectric Point (pI) 7.58

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Gcd14p (InterPro)
Physical Interactions There are 18 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000012B279 | P46959
MIPS: YJL125C
NCBI: 1004315 | 1008322 | 1169870 | 6322336 | NP_012410.1
GenBank/EMBL/DDBJ: DAA08677.1 | Z49400 | Z54149
External Classifications EC: 2.1.1.220 [tRNA (adenine(58)-N(1))-methyltransferase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSTNCFSGYK DLIKEGDLTL IWVSRDNIKP VRMHSEEVFN TRYGSFPHKD
      51  IIGKPYGSQI AIRTKGSNKF AFVHVLQPTP ELWTLSLPHR TQIVYTPDSS
     101  YIMQRLNCSP HSRVIEAGTG SGSFSHAFAR SVGHLFSFEF HHIRYEQALE
     151  EFKEHGLIDD NVTITHRDVC QGGFLIKKGD TTSYEFGNNE TAASLNANVV
     201  FLDLPAPWDA IPHLDSVISV DEKVGLCCFS PCIEQVDKTL DVLEKYGWTD
     251  VEMVEIQGRQ YESRRQMVRS LNDALERLRD IKRHKLQGVE RRKRMFNNTI
     301  DSNDEKVGKR NEDGVPLTEK AKFNPFGKGS RIKEGDSNYK WKEVTKMEAE
     351  IKSHTSYLTF AFKVVNRSRD DEKVNEILRS TEK*

external links for Gcd14p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Gcd14p
1) Cuesta R, et al.  (1998) Identification of GCD14 and GCD15, novel genes required for translational repression of GCN4 mRNA in Saccharomyces cerevisiae. Genetics 148(3):1007-20
2) Kadaba S, et al.  (2004) Nuclear surveillance and degradation of hypomodified initiator tRNAMet in S. cerevisiae. Genes Dev 18(11):1227-40
3) Anderson J, et al.  (2000) The Gcd10p/Gcd14p complex is the essential two-subunit tRNA(1-methyladenosine) methyltransferase of Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 97(10):5173-8
4) Anderson J, et al.  (1998) The essential Gcd10p-Gcd14p nuclear complex is required for 1-methyladenosine modification and maturation of initiator methionyl-tRNA. Genes Dev 12(23):3650-62
5) Hinnebusch A  (1992) "General and Pathway-specific Regulatory Mechanisms Controlling the Synthesis of Amino Acid Biosynthetic Enzymes in Saccharomyces cerevisiae". Pp. 319-414 in The Molecular and Cellular Biology of the Yeast Saccharomyces: Gene Expression, edited by Jones EW, Pringle JR and Broach JR. Cold Spring Harbor, NY: Cold Spring Harbor Laboratory Press
6) Lucchini G, et al.  (1984) Positive regulatory interactions of the HIS4 gene of Saccharomyces cerevisiae. Mol Cell Biol 4(7):1326-33
7) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41