ASF1/YJL115W Protein Information

Standard Name	Asf1p 1
Systematic Name	Yjl115wp
Alias	Cia1p 2
ORF Classification	Verified
Description	Nucleosome assembly factor, involved in chromatin assembly and disassembly, anti-silencing protein that causes derepression of silent loci when overexpressed; plays a role in regulating Ty1 transposition (3, 4, 5, 6, 7)
Name Description	Anti-Silencing Function 8

Experimental Data
Molecules/cell	6230 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	279
Molecular Weight (Da)	31,603
Isoelectric Point (pI)	3.71

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Asf1p (InterPro)
Physical Interactions	There are 157 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005328D \| P32447 MIPS: YJL115W NCBI: 1008304 \| 119389960 \| 126030725 \| 171091 \| 375332299 \| 375332300 \| 375332301 \| 375332302 \| 390981174 \| 40889682 \| 416657 \| 45269639 \| 6322346 \| 71041824 \| NP_012420.1 GenBank/EMBL/DDBJ: DAA08685.1 \| AY557874 \| L07593 \| Z49390

Amino Acid Sequence (or in FASTA format)
1 MSIVSLLGIK VLNNPAKFTD PYEFEITFEC LESLKHDLEW KLTYVGSSRS 51 LDHDQELDSI LVGPVPVGVN KFVFSADPPS AELIPASELV SVTVILLSCS 101 YDGREFVRVG YYVNNEYDEE ELRENPPAKV QVDHIVRNIL AEKPRVTRFN 151 IVWDNENEGD LYPPEQPGVD DEEEEDDEEE DDDEDDEDDE DDDQEDGEGE 201 AEEAAEEEEE EEEKTEDNET NLEEEEEDIE NSDGDEEEGE EEVGSVDKNE 251 DGNDKKRRKI EGGSTDIEST PKDAARSTN*

Amino Acid Sequence (or in FASTA format)

       1  MSIVSLLGIK VLNNPAKFTD PYEFEITFEC LESLKHDLEW KLTYVGSSRS
      51  LDHDQELDSI LVGPVPVGVN KFVFSADPPS AELIPASELV SVTVILLSCS
     101  YDGREFVRVG YYVNNEYDEE ELRENPPAKV QVDHIVRNIL AEKPRVTRFN
     151  IVWDNENEGD LYPPEQPGVD DEEEEDDEEE DDDEDDEDDE DDDQEDGEGE
     201  AEEAAEEEEE EEEKTEDNET NLEEEEEDIE NSDGDEEEGE EEVGSVDKNE
     251  DGNDKKRRKI EGGSTDIEST PKDAARSTN*

external links for Asf1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Asf1p

1)	Sternglanz, R. (1993) Personal Communication, Mortimer Map Edition 12
2)	Yamaki M, et al. (2001) Cell death with predominant apoptotic features in Saccharomyces cerevisiae mediated by deletion of the histone chaperone ASF1/CIA1. Genes Cells 6(12):1043-54
3)	Tyler JK, et al. (1999) The RCAF complex mediates chromatin assembly during DNA replication and repair. Nature 402(6761):555-60
4)	Singer MS, et al. (1998) Identification of high-copy disruptors of telomeric silencing in Saccharomyces cerevisiae. Genetics 150(2):613-32
5)	Osada S, et al. (2001) The yeast SAS (something about silencing) protein complex contains a MYST-type putative acetyltransferase and functions with chromatin assembly factor ASF1. Genes Dev 15(23):3155-68
6)	Schwabish MA and Struhl K (2006) Asf1 mediates histone eviction and deposition during elongation by RNA polymerase II. Mol Cell 22(3):415-22
7)	Nyswaner KM, et al. (2008) Chromatin-associated genes protect the yeast genome from ty1 insertional mutagenesis. Genetics 178(1):197-214
8)	Le S, et al. (1997) Two new S-phase-specific genes from Saccharomyces cerevisiae. Yeast 13(11):1029-42
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41