BCK1/YJL095W Protein Information

Standard Name	Bck1p
Systematic Name	Yjl095wp
Alias	Las3p , Sap3p , Slk1p , Ssp31p
ORF Classification	Verified
Description	Mitogen-activated protein (MAP) kinase kinase kinase acting in the protein kinase C signaling pathway, which controls cell integrity; upon activation by Pkc1p phosphorylates downstream kinases Mkk1p and Mkk2p (1)
Name Description	Bypass of C Kinase

Experimental Data
Molecules/cell	112 2

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	1,478
Molecular Weight (Da)	164,194
Isoelectric Point (pI)	10.3

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bck1p (InterPro)
Physical Interactions	There are 170 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052EAE \| Q01389 MIPS: YJL095W NCBI: 1008270 \| 172074 \| 2131084 \| 218490 \| 3415 \| 417775 \| 6322366 \| 640009 \| NP_012440.1 GenBank/EMBL/DDBJ: DAA08705.1 \| D10389 \| M84389 \| M88604 \| X60227 \| X77923 \| Z49369 \| Z49370
External Classifications	EC: 2.7.-.- [Transferases transferring phosphorous-containing groups]

Amino Acid Sequence (or in FASTA format)
1 MPFLRKIAGT AHTHSRSDSN SSVKFGHQPT SSVASTKSSS KSPRATSRKS 51 IYDDIRSQFP NLTPNSTSSQ FYESTPVIEQ SFNWTTDDHI SAGTLENPTS 101 FTNSSYKNDN GPSSLSDSRK SSGGNSVNSL SFDKLILSWD PTDPDEWTMH 151 RVTSWFKFHD FPESWILFFK KHQLFGHRFI KLLAYDNFAV YEKYLPQTKT 201 ASYTRFQQLL KKTMTKNVTN SHIRQKSASK LKSSRSSSES IKSKLKNSKS 251 QEDISNSRST SESALSPTKS GPSKTDEKNF LHSTSTHQKT KSASSLYRRS 301 FISLRGSSSS NASSAKSPSN IKLSIPARPH SIIESNSTLT KSASPPASPS 351 YPSIFRRHHK SSSSESSLLN SLFGSGIGEE APTKPNPQGH SLSSENLAKG 401 KSKHYETNVS SPLKQSSLPT SDDKGNLWNK FKRKSQIGVP SPNTVAYVTS 451 QETPSLKSNS STATLTVQTA DVNIPSPSSS PPPIPKTANR SLEVISTEDT 501 PKISSTTASF KETYPDCINP DKTVPVPVNN QKYSVKNFLL DQKFYPLKKT 551 GLNDSENKYI LVTKDNVSFV PLNLKSVAKL SSFKESALTK LGINHKNVTF 601 HMTDFDCDIG AAIPDDTLEF LKKSLFLNTS GKIYIKDQMK LQQKPKPAPL 651 TSENNVPLKS VKSKSSMRSG TSSLIASTDD VSIVTSSSDI TSFDEHASGS 701 GRRYPQTPSY YYDRVSNTNP TEELNYWNIK EVLSHEENAP KMVFKTSPKL 751 ELNLPDKGSK LNIPTPITEN ESKSSFQVLR KDEGTEIDFN HRRESPYTKP 801 ELAPKREAPK PPANTSPQRT LSTSKQNKPI RLVRASTKIS RSKRSKPLPP 851 QLLSSPIEAS SSSPDSLTSS YTPASTHVLI PQPYKGANDV MRRLKTDQDS 901 TSTSPSLKMK QKVNRSNSTV STSNSIFYSP SPLLKRGNSK RVVSSTSAAD 951 IFEENDITFA DAPPMFDSDD SDDDSSSSDD IIWSKKKTAP ETNNENKKDE 1001 KSDNSSTHSD EIFYDSQTQD KMERKMTFRP SPEVVYQNLE KFFPRANLDK 1051 PITEGIASPT SPKSLDSLLS PKNVASSRTE PSTPSRPVPP DSSYEFIQDG 1101 LNGKNKPLNQ AKTPKRTKTI RTIAHEASLA RKNSVKLKRQ NTKMWGTRMV 1151 EVTENHMVSI NKAKNSKGEY KEFAWMKGEM IGKGSFGAVY LCLNVTTGEM 1201 MAVKQVEVPK YSSQNEAILS TVEALRSEVS TLKDLDHLNI VQYLGFENKN 1251 NIYSLFLEYV AGGSVGSLIR MYGRFDEPLI KHLTTQVLKG LAYLHSKGIL 1301 HRDMKADNLL LDQDGICKIS DFGISRKSKD IYSNSDMTMR GTVFWMAPEM 1351 VDTKQGYSAK VDIWSLGCIV LEMFAGKRPW SNLEVVAAMF KIGKSKSAPP 1401 IPEDTLPLIS QIGRNFLDAC FEINPEKRPT ANELLSHPFS EVNETFNFKS 1451 TRLAKFIKSN DKLNSSKLRI TSQENKTE*

Amino Acid Sequence (or in FASTA format)

       1  MPFLRKIAGT AHTHSRSDSN SSVKFGHQPT SSVASTKSSS KSPRATSRKS
      51  IYDDIRSQFP NLTPNSTSSQ FYESTPVIEQ SFNWTTDDHI SAGTLENPTS
     101  FTNSSYKNDN GPSSLSDSRK SSGGNSVNSL SFDKLILSWD PTDPDEWTMH
     151  RVTSWFKFHD FPESWILFFK KHQLFGHRFI KLLAYDNFAV YEKYLPQTKT
     201  ASYTRFQQLL KKTMTKNVTN SHIRQKSASK LKSSRSSSES IKSKLKNSKS
     251  QEDISNSRST SESALSPTKS GPSKTDEKNF LHSTSTHQKT KSASSLYRRS
     301  FISLRGSSSS NASSAKSPSN IKLSIPARPH SIIESNSTLT KSASPPASPS
     351  YPSIFRRHHK SSSSESSLLN SLFGSGIGEE APTKPNPQGH SLSSENLAKG
     401  KSKHYETNVS SPLKQSSLPT SDDKGNLWNK FKRKSQIGVP SPNTVAYVTS
     451  QETPSLKSNS STATLTVQTA DVNIPSPSSS PPPIPKTANR SLEVISTEDT
     501  PKISSTTASF KETYPDCINP DKTVPVPVNN QKYSVKNFLL DQKFYPLKKT
     551  GLNDSENKYI LVTKDNVSFV PLNLKSVAKL SSFKESALTK LGINHKNVTF
     601  HMTDFDCDIG AAIPDDTLEF LKKSLFLNTS GKIYIKDQMK LQQKPKPAPL
     651  TSENNVPLKS VKSKSSMRSG TSSLIASTDD VSIVTSSSDI TSFDEHASGS
     701  GRRYPQTPSY YYDRVSNTNP TEELNYWNIK EVLSHEENAP KMVFKTSPKL
     751  ELNLPDKGSK LNIPTPITEN ESKSSFQVLR KDEGTEIDFN HRRESPYTKP
     801  ELAPKREAPK PPANTSPQRT LSTSKQNKPI RLVRASTKIS RSKRSKPLPP
     851  QLLSSPIEAS SSSPDSLTSS YTPASTHVLI PQPYKGANDV MRRLKTDQDS
     901  TSTSPSLKMK QKVNRSNSTV STSNSIFYSP SPLLKRGNSK RVVSSTSAAD
     951  IFEENDITFA DAPPMFDSDD SDDDSSSSDD IIWSKKKTAP ETNNENKKDE
    1001  KSDNSSTHSD EIFYDSQTQD KMERKMTFRP SPEVVYQNLE KFFPRANLDK
    1051  PITEGIASPT SPKSLDSLLS PKNVASSRTE PSTPSRPVPP DSSYEFIQDG
    1101  LNGKNKPLNQ AKTPKRTKTI RTIAHEASLA RKNSVKLKRQ NTKMWGTRMV
    1151  EVTENHMVSI NKAKNSKGEY KEFAWMKGEM IGKGSFGAVY LCLNVTTGEM
    1201  MAVKQVEVPK YSSQNEAILS TVEALRSEVS TLKDLDHLNI VQYLGFENKN
    1251  NIYSLFLEYV AGGSVGSLIR MYGRFDEPLI KHLTTQVLKG LAYLHSKGIL
    1301  HRDMKADNLL LDQDGICKIS DFGISRKSKD IYSNSDMTMR GTVFWMAPEM
    1351  VDTKQGYSAK VDIWSLGCIV LEMFAGKRPW SNLEVVAAMF KIGKSKSAPP
    1401  IPEDTLPLIS QIGRNFLDAC FEINPEKRPT ANELLSHPFS EVNETFNFKS
    1451  TRLAKFIKSN DKLNSSKLRI TSQENKTE*

external links for Bck1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Bck1p

1)	Heinisch JJ, et al. (1999) The protein kinase C-mediated MAP kinase pathway involved in the maintenance of cellular integrity in Saccharomyces cerevisiae. Mol Microbiol 32(4):671-80
2)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41