SRS2/YJL092W Protein Information Help

Standard Name Srs2p 1
Systematic Name Yjl092wp
Alias Hpr5p 2
ORF Classification Verified
Description DNA helicase and DNA-dependent ATPase involved in DNA repair and checkpoint recovery, needed for proper timing of commitment to meiotic recombination and transition from Meiosis I to II; blocks trinucleotide repeat expansion; affects genome stability (10, 3, 4, 5, 6, 7, 8, 9) Also known as: RADH 2 , RADH1
Name Description Suppressor of Rad Six 1
Experimental Data
Molecules/cell 5190 11
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,174
Molecular Weight (Da) 134,305
Isoelectric Point (pI) 9.44

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Srs2p (InterPro)
Physical Interactions There are 142 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000168360 | P12954
MIPS: YJL092W
NCBI: 1008264 | 378792505 | 378792508 | 4277 | 521096 | 6322369 | 83305803 | NP_012443.1 | NM_001181525.1
GenBank/EMBL/DDBJ: DAA08708.1 | X15665 | X77087 | Z49367
External Classifications EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]
EC: 3.6.4.12 [DNA helicase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSSNNDLWLH LVSQLNTQQR AAALFDYTRG LQVIAGPGTG KTKVLTSRVA
      51  YLILHHHIHP RDIIVTTFTN KAANEMKERL QEMLRGAGVN ISELLIGTFH
     101  SICLKILYRF GHLVDLQKDW RIIDEKEIDV ILDDMIEKVP DQIRDYASSI
     151  TRKVNLCMPS KNGDEWTIHP KLIKKQISKL KSNAILPEEY ILDSNHDAAL
     201  GYFYQIYQSE LSKKNTLDFD DLLMYTFRLL TRVRVLSNIK HVLVDEFQDT
     251  NGIQLDLMFL FAKGNHHLSR GMTIVGDPDQ SIYAFRNALA HNFLEMGRKC
     301  PIEYSTIILV ENYRSSQKIL NTSEILITQQ NKGRQNRAPL RAQFDLDFPP
     351  VYMNFPAYFL EAPSLVRELL YLKALPNLFT FNDFAILVRQ RRQIKRIESA
     401  LIEHRIPYKI IRGHSFWDSK ETRAMLNLLK LIFSPNDKHA ILASLLYPAR
     451  GLGPATGEKI KNALDTLATD VSCFQILKDI SSKKIMLDIP TKGRSVIADF
     501  ISMIENCQLL LQSTLLGGLS DLFDKLYELS GLKYEYLYKD GKKKNDQLEK
     551  SEPNLLNARH KNIELLKNYF LALLSKSESS DKEKNEAIKA ATDEAEPIEN
     601  KVITPKEYLR NFFNSLSLHS DAAEEEESES NKDAKIKREK NGFVTISTIH
     651  GAKGLEWPVV FIPGCEEGII PCVFNDDKKD ESEEDEEEDQ ENSKKDASPK
     701  KTRVLSVEDS IDEERRMFFV AQTRAKYLLY LSNTVTVEDV DRPRIASRFL
     751  TTDLIKAMSD SQKLFESTNS IKKLYRILNK KPPAEDDKLF SLDQLRKDYN
     801  QFIENRRERM IWQGIQMNDV YGIQLSRNKL LGSVSDFTSA ADQLRLETQN
     851  SIFPQKKLIE KSRPSKINGN YAPKSRVKSP EKRYAPETTS FHSPTKKKVY
     901  APQYVSTTNV PSRQEFHSST GKNIPFLRRE DRSITDISPR SSTRSLKGAS
     951  PNKTSHMSDD LMRPSPTRKD KVTRNIHFAT AGTFRIETQS NVDELHPPEY
    1001  SNKSGQSLTS SEFSGFSSAC SNSDQPTNLI EDINNELDLS DEELLNDISI
    1051  ERRRELLGSK KTKKIKPKTR NRKSKRGDKV KVEEVIDLKS EFEEDDSRNT
    1101  TAAELLHNPD DTTVDNRPII SNAKFLADAA MKKTQKFSKK VKNEPASSQM
    1151  DIFSQLSRAK KKSKLNNGEI IVID*

external links for Srs2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Srs2p
1) Lawrence CW and Christensen RB  (1979) Metabolic suppressors of trimethoprim and ultraviolet light sensitivities of Saccharomyces cerevisiae rad6 mutants. J Bacteriol 139(3):866-76
2) Rong L, et al.  (1991) The hyper-gene conversion hpr5-1 mutation of Saccharomyces cerevisiae is an allele of the SRS2/RADH gene. Genetics 127(1):75-85
3) Palladino F and Klein HL  (1992) Analysis of mitotic and meiotic defects in Saccharomyces cerevisiae SRS2 DNA helicase mutants. Genetics 132(1):23-37
4) Aboussekhra A, et al.  (1989) RADH, a gene of Saccharomyces cerevisiae encoding a putative DNA helicase involved in DNA repair. Characteristics of radH mutants and sequence of the gene. Nucleic Acids Res 17(18):7211-9
5) Rong L and Klein HL  (1993) Purification and characterization of the SRS2 DNA helicase of the yeast Saccharomyces cerevisiae. J Biol Chem 268(2):1252-9
6) Ubersax JA, et al.  (2003) Targets of the cyclin-dependent kinase Cdk1. Nature 425(6960):859-64
7) Pfander B, et al.  (2005) SUMO-modified PCNA recruits Srs2 to prevent recombination during S phase. Nature 436(7049):428-33
8) Papouli E, et al.  (2005) Crosstalk between SUMO and ubiquitin on PCNA is mediated by recruitment of the helicase Srs2p. Mol Cell 19(1):123-33
9) Dhar A and Lahue RS  (2008) Rapid unwinding of triplet repeat hairpins by Srs2 helicase of Saccharomyces cerevisiae. Nucleic Acids Res 36(10):3366-73
10) Yeung M and Durocher D  (2011) Srs2 enables checkpoint recovery by promoting disassembly of DNA damage foci from chromatin. DNA Repair (Amst) 10(12):1213-22
11) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41