TRL1/YJL087C Protein Information Help

Standard Name Trl1p
Systematic Name Yjl087cp
Alias Lig1p , Rlg1p 1
ORF Classification Verified
Description tRNA ligase, required for tRNA splicing and for both splicing and translation of HAC1 mRNA in the UPR; has phosphodiesterase, polynucleotide kinase, and ligase activities; localized at the inner nuclear envelope and partially to polysomes (2, 3, 4)
Name Description tRNA Ligase
Experimental Data
Molecules/cell 3110 5
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 827
Molecular Weight (Da) 95,336
Isoelectric Point (pI) 7.76

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Trl1p (InterPro)
Physical Interactions There are 4 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013749C | P09880
MIPS: YJL087C
NCBI: 1008252 | 136250 | 171844 | 6322374 | 929868 | NP_012448.1
GenBank/EMBL/DDBJ: DAA08713.1 | J03546 | X83502 | Z49362
External Classifications EC: 6.5.1.3 [RNA ligase (ATP)]
Amino Acid Sequence (or in FASTA format)
 
       1  MPSPYDGKRT VTQLVNELEK AEKLSGRGRA YRRVCDLSHS NKKVISWKFN
      51  EWDYGKNTIT LPCNARGLFI SDDTTNPVIV ARGYDKFFNV GEVNFTKWNW
     101  IEENCTGPYD VTIKANGCII FISGLEDGTL VVCSKHSTGP RADVDRNHAE
     151  AGEKQLLRQL AAMNINRSDF ARMLYTHNVT AVAEYCDDSF EEHILEYPLE
     201  KAGLYLHGVN VNKAEFETWD MKDVSQMASK YGFRCVQCIT SNTLEDLKKF
     251  LDNCSATGSF EGQEIEGFVI RCHLKSTEKP FFFKYKFEEP YLMYRQWREV
     301  TKDYISNKSR VFKFRKHKFI TNKYLDFAIP ILESSPKICE NYLKGFGVIE
     351  LRNKFLQSYG MSGLEILNHE KVAELELKNA IDYDKVDERT KFLIFPISVI
     401  GCGKTTTSQT LVNLFPDSWG HIQNDDITGK DKSQLMKKSL ELLSKKEIKC
     451  VIVDRNNHQF RERKQLFEWL NELKEDYLVY DTNIKVIGVS FAPYDKLSEI
     501  RDITLQRVIK RGNNHQSIKW DELGEKKVVG IMNGFLKRYQ PVNLDKSPDN
     551  MFDLMIELDF GQADSSLTNA KQILNEIHKA YPILVPEIPK DDEIETAFRR
     601  SLDYKPTVRK IVGKGNNNQQ KTPKLIKPTY ISAKIENYDE IIELVKRCIA
     651  SDAELTEKFK HLLASGKVQK ELHITLGHVM SSREKEAKKL WKSYCNRYTD
     701  QITEYNNNRI ENAQGSGNNQ NTQVKTTDKL NFRLEKLCWD EKIIAIVVEL
     751  SKDKDGCIID ENNEKIKGLC CQNKIPHITL CKLESGVKAV YSNVLCEKVE
     801  SAEVDENIKV VKLDNSKEFV GSVYLNF*

external links for Trl1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA

References cited on this page View Complete Literature Guide for Trl1p
1) Baymiller J, et al.  (1994) Isolation and sequence of the t-RNA ligase-encoding gene of Candida albicans. Gene 142(1):129-34
2) Xu Q, et al.  (1990) Domain structure in yeast tRNA ligase. Biochemistry 29(26):6132-8
3) Clark MW and Abelson J  (1987) The subnuclear localization of tRNA ligase in yeast. J Cell Biol 105(4):1515-26
4) Mori T, et al.  (2010) Dual Functions of Yeast tRNA Ligase in the Unfolded Protein Response: Unconventional Cytoplasmic Splicing of HAC1 Pre-mRNA Is Not Sufficient to Release Translational Attenuation. Mol Biol Cell 21(21):3722-34
5) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41