NUP82/YJL061W Protein Information

Standard Name	Nup82p 1
Systematic Name	Yjl061wp
Alias	Hrb187p
ORF Classification	Verified
Description	Linker nucleoporin component of the nuclear pore complex (NPC); also part of the NPC cytoplasmic filaments; contributes to nucleocytoplasmic transport and NPC biogenesis; forms stable associations with three FG-nucleoporins (Nsp1p, Nup159p, and Nup116p) (2, 3, 4, 5, 6, 7, 8)
Name Description	NUclear Pore 1

Experimental Data
Molecules/cell	9470 9

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	713
Molecular Weight (Da)	82,085
Isoelectric Point (pI)	5.09

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Nup82p (InterPro)
Physical Interactions	There are 109 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000053066 \| P40368 MIPS: YJL061W NCBI: 1008207 \| 1165143 \| 1171853 \| 354459533 \| 354459536 \| 354459539 \| 354459542 \| 383875491 \| 383875494 \| 383875497 \| 499006 \| 6322400 \| 961605 \| NP_012474.1 GenBank/EMBL/DDBJ: DAA08737.1 \| U31543 \| X85970 \| Z34288 \| Z49336

Amino Acid Sequence (or in FASTA format)
1 MSQSSRLSAL PIFQASLSAS QSPRYIFSSQ NGTRIVFIQD NIIRWYNVLT 51 DSLYHSLNFS RHLVLDDTFH VISSTSGDLL CLFNDNEIFV MEVPWGYSNV 101 EDVSIQDAFQ IFHYSIDEEE VGPKSSIKKV LFHPKSYRDS CIVVLKEDDT 151 ITMFDILNSQ EKPIVLNKPN NSFGLDARVN DITDLEFSKD GLTLYCLNTT 201 EGGDIFAFYP FLPSVLLLNE KDLNLILNKS LVMYESLDST TDVIVKRNVI 251 KQLQFVSKLH ENWNSRFGKV DIQKEYRLAK VQGPFTINPF PGELYDYTAT 301 NIATILIDNG QNEIVCVSFD DGSLILLFKD LEMSMSWDVD NYVYNNSLVL 351 IERVKLQREI KSLITLPEQL GKLYVISDNI IQQVNFMSWA STLSKCINES 401 DLNPLAGLKF ESKLEDIATI ERIPNLAYIN WNDQSNLALM SNKTLTFQNI 451 SSDMKPQSTA AETSISTEKS DTVGDGFKMS FTQPINEILI LNDNFQKACI 501 SPCERIIPSA DRQIPLKNEA SENQLEIFTD ISKEFLQRIV KAQTLGVSIH 551 NRIHEQQFEL TRQLQSTCKI ISKDDDLRRK FEAQNKKWDA QLSRQSELME 601 RFSKLSKKLS QIAESNKFKE KKISHGEMKW FKEIRNQILQ FNSFVHSQKS 651 LQQDLSYLKS ELTRIEAETI KVDKKSQNEW DELRKMLEID SKIIKECNEE 701 LLQVSQEFTT KTQ*

Amino Acid Sequence (or in FASTA format)

       1  MSQSSRLSAL PIFQASLSAS QSPRYIFSSQ NGTRIVFIQD NIIRWYNVLT
      51  DSLYHSLNFS RHLVLDDTFH VISSTSGDLL CLFNDNEIFV MEVPWGYSNV
     101  EDVSIQDAFQ IFHYSIDEEE VGPKSSIKKV LFHPKSYRDS CIVVLKEDDT
     151  ITMFDILNSQ EKPIVLNKPN NSFGLDARVN DITDLEFSKD GLTLYCLNTT
     201  EGGDIFAFYP FLPSVLLLNE KDLNLILNKS LVMYESLDST TDVIVKRNVI
     251  KQLQFVSKLH ENWNSRFGKV DIQKEYRLAK VQGPFTINPF PGELYDYTAT
     301  NIATILIDNG QNEIVCVSFD DGSLILLFKD LEMSMSWDVD NYVYNNSLVL
     351  IERVKLQREI KSLITLPEQL GKLYVISDNI IQQVNFMSWA STLSKCINES
     401  DLNPLAGLKF ESKLEDIATI ERIPNLAYIN WNDQSNLALM SNKTLTFQNI
     451  SSDMKPQSTA AETSISTEKS DTVGDGFKMS FTQPINEILI LNDNFQKACI
     501  SPCERIIPSA DRQIPLKNEA SENQLEIFTD ISKEFLQRIV KAQTLGVSIH
     551  NRIHEQQFEL TRQLQSTCKI ISKDDDLRRK FEAQNKKWDA QLSRQSELME
     601  RFSKLSKKLS QIAESNKFKE KKISHGEMKW FKEIRNQILQ FNSFVHSQKS
     651  LQQDLSYLKS ELTRIEAETI KVDKKSQNEW DELRKMLEID SKIIKECNEE
     701  LLQVSQEFTT KTQ*

external links for Nup82p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Nup82p

1)	Grandi P, et al. (1995) A novel nuclear pore protein Nup82p which specifically binds to a fraction of Nsp1p. J Cell Biol 130(6):1263-73
2)	Ho AK, et al. (2000) Assembly and preferential localization of Nup116p on the cytoplasmic face of the nuclear pore complex by interaction with Nup82p. Mol Cell Biol 20(15):5736-48
3)	Rout MP, et al. (2000) The yeast nuclear pore complex: composition, architecture, and transport mechanism. J Cell Biol 148(4):635-51
4)	Hurwitz ME, et al. (1998) Two yeast nuclear pore complex proteins involved in mRNA export form a cytoplasmically oriented subcomplex. Proc Natl Acad Sci U S A 95(19):11241-5
5)	Belanger KD, et al. (2004) The karyopherin Msn5/Kap142 requires Nup82 for nuclear export and performs a function distinct from translocation in RPA protein import. J Biol Chem 279(42):43530-9
6)	Lutzmann M, et al. (2005) Reconstitution of Nup157 and Nup145N into the Nup84 complex. J Biol Chem 280(18):18442-51
7)	West M, et al. (2007) Novel interaction of the 60S ribosomal subunit export adapter Nmd3 at the nuclear pore complex. J Biol Chem 282(19):14028-37
8)	Alber F, et al. (2007) The molecular architecture of the nuclear pore complex. Nature 450(7170):695-701
9)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41