TDH1/YJL052W Protein Information

Standard Name	Tdh1p 1
Systematic Name	Yjl052wp
Alias	Gld3p
ORF Classification	Verified
Description	Glyceraldehyde-3-phosphate dehydrogenase, isozyme 1; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; protein abundance increases in response to DNA replication stress (1, 2, 3)
Name Description	Triose-phosphate DeHydrogenase 1
Gene Product	GAPDH 4

Experimental Data
Molecules/cell	120000 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	332
Molecular Weight (Da)	35,750
Isoelectric Point (pI)	8.59

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tdh1p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 48 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000168364 \| P00360 MIPS: YJL052W NCBI: 1008189 \| 1169786 \| 3726 \| 398364523 \| 51013453 \| NP_012483.3 \| NM_001181485.3 GenBank/EMBL/DDBJ: DAA08747.1 \| AY693001 \| V01302 \| Z49327
External Classifications	EC: 1.2.1.12 [Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)]

Amino Acid Sequence (or in FASTA format)
1 MIRIAINGFG RIGRLVLRLA LQRKDIEVVA VNDPFISNDY AAYMVKYDST 51 HGRYKGTVSH DDKHIIIDGV KIATYQERDP ANLPWGSLKI DVAVDSTGVF 101 KELDTAQKHI DAGAKKVVIT APSSSAPMFV VGVNHTKYTP DKKIVSNASC 151 TTNCLAPLAK VINDAFGIEE GLMTTVHSMT ATQKTVDGPS HKDWRGGRTA 201 SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLEKE 251 ATYDQIKKAV KAAAEGPMKG VLGYTEDAVV SSDFLGDTHA SIFDASAGIQ 301 LSPKFVKLIS WYDNEYGYSA RVVDLIEYVA KA*

Amino Acid Sequence (or in FASTA format)

       1  MIRIAINGFG RIGRLVLRLA LQRKDIEVVA VNDPFISNDY AAYMVKYDST
      51  HGRYKGTVSH DDKHIIIDGV KIATYQERDP ANLPWGSLKI DVAVDSTGVF
     101  KELDTAQKHI DAGAKKVVIT APSSSAPMFV VGVNHTKYTP DKKIVSNASC
     151  TTNCLAPLAK VINDAFGIEE GLMTTVHSMT ATQKTVDGPS HKDWRGGRTA
     201  SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLEKE
     251  ATYDQIKKAV KAAAEGPMKG VLGYTEDAVV SSDFLGDTHA SIFDASAGIQ
     301  LSPKFVKLIS WYDNEYGYSA RVVDLIEYVA KA*

external links for Tdh1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
YGOB	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Tdh1p

1)	McAlister L and Holland MJ (1985) Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3-phosphate dehydrogenase genes. J Biol Chem 260(28):15013-8
2)	McAlister L and Holland MJ (1985) Differential expression of the three yeast glyceraldehyde-3-phosphate dehydrogenase genes. J Biol Chem 260(28):15019-27
3)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
4)	Delgado ML, et al. (2001) The glyceraldehyde-3-phosphate dehydrogenase polypeptides encoded by the Saccharomyces cerevisiae TDH1, TDH2 and TDH3 genes are also cell wall proteins. Microbiology 147(Pt 2):411-7
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41