MTR4/YJL050W Protein Information Help

Standard Name Mtr4p 1
Systematic Name Yjl050wp
Alias Dob1p 2
ORF Classification Verified
Description ATP-dependent 3'-5' RNA helicase of the DExD/H family; involved in nuclear RNA processing and degradation both as a component of TRAMP complex and in TRAMP-independent processes; TRAMP unwinds RNA duplexes, with Mtr4p unwinding activity stimulated by Pap2p/Air2p but not dependent on ongoing polyadenylation; contains an arch domain, with two coiled-coil arms/stalks and a globular fist/KOW domain, which has RNA binding activity and is required for 5.8S rRNA processing (10, 11, 2, 3, 4, 5, 6, 7, 8, 9)
Name Description Mrna TRansport 12
Experimental Data
Molecules/cell 12500 13
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,073
Molecular Weight (Da) 122,054
Isoelectric Point (pI) 6.28

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mtr4p (InterPro)
Physical Interactions There are 86 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052EB1 | P47047
MIPS: YJL050W
NCBI: 1008185 | 1352980 | 297787672 | 299688859 | 299688860 | 6322411 | NP_012485.1
GenBank/EMBL/DDBJ: DAA08749.1 | Z49325
External Classifications EC: 3.6.1.- [Hydrolases acting on acid anhydrides in phosphorous-containing anhydrides]
EC: 3.6.4.13 [RNA helicase]
Amino Acid Sequence (or in FASTA format)
 
       1  MDSTDLFDVF EETPVELPTD SNGEKNADTN VGDTPDHTQD KKHGLEEEKE
      51  EHEENNSENK KIKSNKSKTE DKNKKVVVPV LADSFEQEAS REVDASKGLT
     101  NSETLQVEQD GKVRLSHQVR HQVALPPNYD YTPIAEHKRV NEARTYPFTL
     151  DPFQDTAISC IDRGESVLVS AHTSAGKTVV AEYAIAQSLK NKQRVIYTSP
     201  IKALSNQKYR ELLAEFGDVG LMTGDITINP DAGCLVMTTE ILRSMLYRGS
     251  EVMREVAWVI FDEVHYMRDK ERGVVWEETI ILLPDKVRYV FLSATIPNAM
     301  EFAEWICKIH SQPCHIVYTN FRPTPLQHYL FPAHGDGIYL VVDEKSTFRE
     351  ENFQKAMASI SNQIGDDPNS TDSRGKKGQT YKGGSAKGDA KGDIYKIVKM
     401  IWKKKYNPVI VFSFSKRDCE ELALKMSKLD FNSDDEKEAL TKIFNNAIAL
     451  LPETDRELPQ IKHILPLLRR GIGIHHSGLL PILKEVIEIL FQEGFLKVLF
     501  ATETFSIGLN MPAKTVVFTS VRKWDGQQFR WVSGGEYIQM SGRAGRRGLD
     551  DRGIVIMMID EKMEPQVAKG MVKGQADRLD SAFHLGYNMI LNLMRVEGIS
     601  PEFMLEHSFF QFQNVISVPV MEKKLAELKK DFDGIEVEDE ENVKEYHEIE
     651  QAIKGYREDV RQVVTHPANA LSFLQPGRLV EISVNGKDNY GWGAVVDFAK
     701  RINKRNPSAV YTDHESYIVN VVVNTMYIDS PVNLLKPFNP TLPEGIRPAE
     751  EGEKSICAVI PITLDSIKSI GNLRLYMPKD IRASGQKETV GKSLREVNRR
     801  FPDGIPVLDP VKNMKIEDED FLKLMKKIDV LNTKLSSNPL TNSMRLEELY
     851  GKYSRKHDLH EDMKQLKRKI SESQAVIQLD DLRRRKRVLR RLGFCTPNDI
     901  IELKGRVACE ISSGDELLLT ELIFNGNFNE LKPEQAAALL SCFAFQERCK
     951  EAPRLKPELA EPLKAMREIA AKIAKIMKDS KIEVVEKDYV ESFRHELMEV
    1001  VYEWCRGATF TQICKMTDVY EGSLIRMFKR LEELVKELVD VANTIGNSSL
    1051  KEKMEAVLKL IHRDIVSAGS LYL*

external links for Mtr4p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Mtr4p
1) Liang S, et al.  (1996) A DEAD-box-family protein is required for nucleocytoplasmic transport of yeast mRNA. Mol Cell Biol 16(9):5139-46
2) de la Cruz J, et al.  (1998) Dob1p (Mtr4p) is a putative ATP-dependent RNA helicase required for the 3' end formation of 5.8S rRNA in Saccharomyces cerevisiae. EMBO J 17(4):1128-40
3) Vanacova S, et al.  (2005) A new yeast poly(A) polymerase complex involved in RNA quality control. PLoS Biol 3(6):e189
4) LaCava J, et al.  (2005) RNA degradation by the exosome is promoted by a nuclear polyadenylation complex. Cell 121(5):713-24
5) Wyers F, et al.  (2005) Cryptic pol II transcripts are degraded by a nuclear quality control pathway involving a new poly(A) polymerase. Cell 121(5):725-37
6) Wang X, et al.  (2008) Degradation of hypomodified tRNA(iMet) in vivo involves RNA-dependent ATPase activity of the DExH helicase Mtr4p. RNA 14(1):107-16
7) Bernstein J, et al.  (2008) Characterization of the Essential Activities of Saccharomyces cerevisiae Mtr4p, a 3'->5' Helicase Partner of the Nuclear Exosome. J Biol Chem 283(8):4930-42
8) Lebreton A and Seraphin B  (2008) Exosome-mediated quality control: substrate recruitment and molecular activity. Biochim Biophys Acta 1779(9):558-65
9) Jackson RN, et al.  (2010) The crystal structure of Mtr4 reveals a novel arch domain required for rRNA processing. EMBO J 29(13):2205-16
10) Weir JR, et al.  (2010) Structural analysis reveals the characteristic features of Mtr4, a DExH helicase involved in nuclear RNA processing and surveillance. Proc Natl Acad Sci U S A 107(27):12139-44
11) Jia H, et al.  (2012) RNA unwinding by the Trf4/Air2/Mtr4 polyadenylation (TRAMP) complex. Proc Natl Acad Sci U S A 109(19):7292-7
12) Kadowaki T, et al.  (1994) Isolation and characterization of Saccharomyces cerevisiae mRNA transport-defective (mtr) mutants. J Cell Biol 126(3):649-59
13) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41