KAR2/YJL034W Protein Information Help

Standard Name Kar2p
Systematic Name Yjl034wp
Alias Grp78p
ORF Classification Verified
Description ATPase involved in protein import into the ER, also acts as a chaperone to mediate protein folding in the ER and may play a role in ER export of soluble proteins; regulates the unfolded protein response via interaction with Ire1p (1, 2, 3)
Name Description KARyogamy
Gene Product BIP
Experimental Data
Molecules/cell 337000 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 682
Molecular Weight (Da) 74,467
Isoelectric Point (pI) 4.62

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Kar2p (InterPro)
Transmembrane Domains There are 1 total predicted transmembrane domains (TMHMM)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 148 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000036C61 | P16474
MIPS: YJL034W
NCBI: 1008157 | 121575 | 171130 | 171771 | 171773 | 227343891 | 340707865 | 340707866 | 340707898 | 340707899 | 398364585 | NP_012500.3
GenBank/EMBL/DDBJ: DAA08765.1 | M25064 | M25394 | M31006 | Z49309
Amino Acid Sequence (or in FASTA format)
 
       1  MFFNRLSAGK LLVPLSVVLY ALFVVILPLQ NSFHSSNVLV RGADDVENYG
      51  TVIGIDLGTT YSCVAVMKNG KTEILANEQG NRITPSYVAF TDDERLIGDA
     101  AKNQVAANPQ NTIFDIKRLI GLKYNDRSVQ KDIKHLPFNV VNKDGKPAVE
     151  VSVKGEKKVF TPEEISGMIL GKMKQIAEDY LGTKVTHAVV TVPAYFNDAQ
     201  RQATKDAGTI AGLNVLRIVN EPTAAAIAYG LDKSDKEHQI IVYDLGGGTF
     251  DVSLLSIENG VFEVQATSGD THLGGEDFDY KIVRQLIKAF KKKHGIDVSD
     301  NNKALAKLKR EAEKAKRALS SQMSTRIEID SFVDGIDLSE TLTRAKFEEL
     351  NLDLFKKTLK PVEKVLQDSG LEKKDVDDIV LVGGSTRIPK VQQLLESYFD
     401  GKKASKGINP DEAVAYGAAV QAGVLSGEEG VEDIVLLDVN ALTLGIETTG
     451  GVMTPLIKRN TAIPTKKSQI FSTAVDNQPT VMIKVYEGER AMSKDNNLLG
     501  KFELTGIPPA PRGVPQIEVT FALDANGILK VSATDKGTGK SESITITNDK
     551  GRLTQEEIDR MVEEAEKFAS EDASIKAKVE SRNKLENYAH SLKNQVNGDL
     601  GEKLEEEDKE TLLDAANDVL EWLDDNFETA IAEDFDEKFE SLSKVAYPIT
     651  SKLYGGADGS GAADYDDEDE DDDGDYFEHD EL*

external links for Kar2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Aspergillus (AspGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CGD) CYC2008 (complexes) Pfam domains
Candida (CandidaDB) Complexome YeastRC Structure Prediction (Seattle)
YGOB DIP

YOGY GeneMANIA


YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Kar2p
1) Romisch K  (1999) Surfing the Sec61 channel: bidirectional protein translocation across the ER membrane. J Cell Sci 112 ( Pt 23)():4185-91
2) Tokunaga M, et al.  (1992) Purification and characterization of BiP/Kar2 protein from Saccharomyces cerevisiae. J Biol Chem 267(25):17553-9
3) Okamura K, et al.  (2000) Dissociation of Kar2p/BiP from an ER sensory molecule, Ire1p, triggers the unfolded protein response in yeast. Biochem Biophys Res Commun 279(2):445-50
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41