MAD2/YJL030W Protein Information

Standard Name	Mad2p
Systematic Name	Yjl030wp
ORF Classification	Verified
Description	Component of the spindle-assembly checkpoint complex; delays the onset of anaphase in cells with defects in mitotic spindle assembly; forms a complex with Mad1p; regulates APC/C activity during prometaphase and metaphase of meiosis I (1, 2)
Name Description	Mitotic Arrest-Deficient 1

Experimental Data
Molecules/cell	1110 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	196
Molecular Weight (Da)	22,284
Isoelectric Point (pI)	5.15

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Mad2p (InterPro)
Physical Interactions	There are 69 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012EB17 \| P40958 MIPS: YJL030W NCBI: 1008150 \| 398364605 \| 45270376 \| 537309 \| 729977 \| NP_012504.3 GenBank/EMBL/DDBJ: DAA08768.1 \| AY558243 \| U14132 \| Z49305

Amino Acid Sequence (or in FASTA format)
1 MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT 51 HDDELKDYIR KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH 101 ISGNSNGQDD VVDLNTTQSQ IRALIRQITS SVTFLPELTK EGGYTFTVLA 151 YTDADAKVPL EWADSNSKEI PDGEVVQFKT FSTNDHKVGA QVSYKY*

Amino Acid Sequence (or in FASTA format)

       1  MSQSISLKGS TRTVTEFFEY SINSILYQRG VYPAEDFVTV KKYDLTLLKT
      51  HDDELKDYIR KILLQVHRWL LGGKCNQLVL CIVDKDEGEV VERWSFNVQH
     101  ISGNSNGQDD VVDLNTTQSQ IRALIRQITS SVTFLPELTK EGGYTFTVLA
     151  YTDADAKVPL EWADSNSKEI PDGEVVQFKT FSTNDHKVGA QVSYKY*

external links for Mad2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Mad2p

1)	Li R and Murray AW (1991) Feedback control of mitosis in budding yeast. Cell 66(3):519-31
2)	Tsuchiya D, et al. (2011) The spindle checkpoint protein Mad2 regulates APC/C activity during prometaphase and metaphase of meiosis I in Saccharomyces cerevisiae. Mol Biol Cell 22(16):2848-61
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41