VTC4/YJL012C Protein Information

Standard Name	Vtc4p 1
Systematic Name	Yjl012cp
Alias	Phm3p 2 , Yjl012c-ap
ORF Classification	Verified
Description	Vacuolar membrane polyphosphate polymerase; subunit of the vacuolar transporter chaperone (VTC) complex involved in synthesis and transfer of polyP to the vacuole; regulates membrane trafficking; role in non-autophagic vacuolar fusion; protein abundance increases in response to DNA replication stress (1, 2, 3, 4, 5, 6, 7)
Name Description	Vacuolar Transporter Chaperone 1

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	721
Molecular Weight (Da)	83,154
Isoelectric Point (pI)	6.81

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Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Vtc4p (InterPro)
Transmembrane Domains	There are 3 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 22 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000191CD5 \| P47075 MIPS: YJL012C NCBI: 1006727 \| 229597872 \| 229597873 \| 229597874 \| 229597875 \| 229597876 \| 229597877 \| 229597878 \| 30267875 \| 33391164 \| 42742256 \| 51704292 \| NP_012522.2 GenBank/EMBL/DDBJ: DAA08781.1 \| AY227894 \| AY264259 \| Z49287

Amino Acid Sequence (or in FASTA format)
1 MKFGEHLSKS LIRQYSYYYI SYDDLKTELE DNLSKNNGQW TQELETDFLE 51 SLEIELDKVY TFCKVKHSEV FRRVKEVQEQ VQHTVRLLDS NNPPTQLDFE 101 ILEEELSDII ADVHDLAKFS RLNYTGFQKI IKKHDKKTGF ILKPVFQVRL 151 DSKPFFKENY DELVVKISQL YDIARTSGRP IKGDSSAGGK QQNFVRQTTK 201 YWVHPDNITE LKLIILKHLP VLVFNTNKEF EREDSAITSI YFDNENLDLY 251 YGRLRKDEGA EAHRLRWYGG MSTDTIFVER KTHREDWTGE KSVKARFALK 301 ERHVNDFLKG KYTVDQVFAK MRKEGKKPMN EIENLEALAS EIQYVMLKKK 351 LRPVVRSFYN RTAFQLPGDA RVRISLDTEL TMVREDNFDG VDRTHKNWRR 401 TDIGVDWPFK QLDDKDICRF PYAVLEVKLQ TQLGQEPPEW VRELVGSHLV 451 EPVPKFSKFI HGVATLLNDK VDSIPFWLPQ MDVDIRKPPL PTNIEITRPG 501 RSDNEDNDFD EDDEDDAALV AAMTNAPGNS LDIEESVGYG ATSAPTSNTN 551 HVVESANAAY YQRKIRNAEN PISKKYYEIV AFFDHYFNGD QISKIPKGTT 601 FDTQIRAPPG KTICVPVRVE PKVYFATERT YLSWLSISIL LGGVSTTLLT 651 YGSPTAMIGS IGFFITSLAV LIRTVMVYAK RVVNIRLKRA VDYEDKIGPG 701 MVSVFLILSI LFSFFCNLVA K*

Amino Acid Sequence (or in FASTA format)

       1  MKFGEHLSKS LIRQYSYYYI SYDDLKTELE DNLSKNNGQW TQELETDFLE
      51  SLEIELDKVY TFCKVKHSEV FRRVKEVQEQ VQHTVRLLDS NNPPTQLDFE
     101  ILEEELSDII ADVHDLAKFS RLNYTGFQKI IKKHDKKTGF ILKPVFQVRL
     151  DSKPFFKENY DELVVKISQL YDIARTSGRP IKGDSSAGGK QQNFVRQTTK
     201  YWVHPDNITE LKLIILKHLP VLVFNTNKEF EREDSAITSI YFDNENLDLY
     251  YGRLRKDEGA EAHRLRWYGG MSTDTIFVER KTHREDWTGE KSVKARFALK
     301  ERHVNDFLKG KYTVDQVFAK MRKEGKKPMN EIENLEALAS EIQYVMLKKK
     351  LRPVVRSFYN RTAFQLPGDA RVRISLDTEL TMVREDNFDG VDRTHKNWRR
     401  TDIGVDWPFK QLDDKDICRF PYAVLEVKLQ TQLGQEPPEW VRELVGSHLV
     451  EPVPKFSKFI HGVATLLNDK VDSIPFWLPQ MDVDIRKPPL PTNIEITRPG
     501  RSDNEDNDFD EDDEDDAALV AAMTNAPGNS LDIEESVGYG ATSAPTSNTN
     551  HVVESANAAY YQRKIRNAEN PISKKYYEIV AFFDHYFNGD QISKIPKGTT
     601  FDTQIRAPPG KTICVPVRVE PKVYFATERT YLSWLSISIL LGGVSTTLLT
     651  YGSPTAMIGS IGFFITSLAV LIRTVMVYAK RVVNIRLKRA VDYEDKIGPG
     701  MVSVFLILSI LFSFFCNLVA K*

external links for Vtc4p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Vtc4p

1)	Cohen A, et al. (1999) A novel family of yeast chaperons involved in the distribution of V-ATPase and other membrane proteins. J Biol Chem 274(38):26885-93
2)	Ogawa N, et al. (2000) New components of a system for phosphate accumulation and polyphosphate metabolism in Saccharomyces cerevisiae revealed by genomic expression analysis. Mol Biol Cell 11(12):4309-21
3)	Muller O, et al. (2002) The Vtc proteins in vacuole fusion: coupling NSF activity to V(0) trans-complex formation. EMBO J 21(3):259-69
4)	Muller O, et al. (2003) Role of the Vtc proteins in V-ATPase stability and membrane trafficking. J Cell Sci 116(Pt 6):1107-15
5)	Uttenweiler A, et al. (2007) The vacuolar transporter chaperone (VTC) complex is required for microautophagy. Mol Biol Cell 18(1):166-75
6)	Hothorn M, et al. (2009) Catalytic core of a membrane-associated eukaryotic polyphosphate polymerase. Science 324(5926):513-6
7)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76