PRE3/YJL001W Protein Information

Standard Name	Pre3p
Systematic Name	Yjl001wp
Alias	Crl21p
ORF Classification	Verified
Description	Beta 1 subunit of the 20S proteasome, responsible for cleavage after acidic residues in peptides (1, 2)
Name Description	PRoteinase yscE

Experimental Data
Molecules/cell	7250 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	215
Molecular Weight (Da)	23,547
Isoelectric Point (pI)	5.73

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pre3p (InterPro)
Physical Interactions	There are 80 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FDC \| P38624 MIPS: YJL001W NCBI: 1041974 \| 112490856 \| 112490870 \| 11513426 \| 11513440 \| 11514005 \| 11514019 \| 1172606 \| 14488812 \| 14488826 \| 178847473 \| 178847487 \| 178847515 \| 178847529 \| 190016380 \| 190016394 \| 197725334 \| 197725348 \| 20150390 \| 20150397 \| 211939225 \| 211939239 \| 211939253 \| 211939267 \| 2131077 \| 2131078 \| 238828250 \| 238828264 \| 258588334 \| 258588348 \| 258588362 \| 258588376 \| 258588556 \| 258588570 \| 298508229 \| 298508243 \| 298508445 \| 298508459 \| 3114276 \| 3114290 \| 323462949 \| 323462963 \| 323462977 \| 323462991 \| 323463005 \| 323463019 \| 330689394 \| 330689408 \| 333361157 \| 333361171 \| 333361185 \| 333361199 \| 333361213 \| 333361227 \| 333361241 \| 333361255 \| 335892354 \| 335892368 \| 340780432 \| 340780446 \| 340780460 \| 340780474 \| 350610685 \| 350610699 \| 359545859 \| 359545873 \| 378792254 \| 378792288 \| 390980829 \| 390980843 \| 390980857 \| 390980871 \| 402550745 \| 403071954 \| 403072198 \| 409973873 \| 409973901 \| 444302352 \| 444302380 \| 444302408 \| 476044 \| 6322459 \| 75765706 \| 75765720 \| 780279 \| 854572 \| 93279381 \| 93279395 \| 99032215 \| 99032229 \| NP_012533.1 GenBank/EMBL/DDBJ: DAA08790.1 \| S78566 \| X78991 \| X86020 \| X87611 \| Z49276 \| Z49277
External Classifications	EC: 3.4.25.1 [Proteasome endopeptidase complex]

Amino Acid Sequence (or in FASTA format)
1 MNGIQVDINR LKKGEVSLGT SIMAVTFKDG VILGADSRTT TGAYIANRVT 51 DKLTRVHDKI WCCRSGSAAD TQAIADIVQY HLELYTSQYG TPSTETAASV 101 FKELCYENKD NLTAGIIVAG YDDKNKGEVY TIPLGGSVHK LPYAIAGSGS 151 TFIYGYCDKN FRENMSKEET VDFIKHSLSQ AIKWDGSSGG VIRMVVLTAA 201 GVERLIFYPD EYEQL*

Amino Acid Sequence (or in FASTA format)

       1  MNGIQVDINR LKKGEVSLGT SIMAVTFKDG VILGADSRTT TGAYIANRVT
      51  DKLTRVHDKI WCCRSGSAAD TQAIADIVQY HLELYTSQYG TPSTETAASV
     101  FKELCYENKD NLTAGIIVAG YDDKNKGEVY TIPLGGSVHK LPYAIAGSGS
     151  TFIYGYCDKN FRENMSKEET VDFIKHSLSQ AIKWDGSSGG VIRMVVLTAA
     201  GVERLIFYPD EYEQL*

external links for Pre3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Mass Spec (Seattle)
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pre3p

1)	Groll M, et al. (1999) The catalytic sites of 20S proteasomes and their role in subunit maturation: a mutational and crystallographic study. Proc Natl Acad Sci U S A 96(20):10976-83
2)	Jager S, et al. (1999) Proteasome beta-type subunits: unequal roles of propeptides in core particle maturation and a hierarchy of active site function. J Mol Biol 291(4):997-1013
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41