BIO2/YGR286C Protein Information

Standard Name	Bio2p
Systematic Name	Ygr286cp
ORF Classification	Verified
Description	Biotin synthase, catalyzes the conversion of dethiobiotin to biotin, which is the last step of the biotin biosynthesis pathway; complements E. coli bioB mutant (1)
Name Description	BIOtin 1

Experimental Data
Molecules/cell	504 2

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	375
Molecular Weight (Da)	41,884
Isoelectric Point (pI)	8.54

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bio2p (InterPro)
Physical Interactions	There are 2 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000126966 \| P32451 MIPS: YGR286C NCBI: 1323523 \| 1705465 \| 288357 \| 6321725 \| NP_011802.1 GenBank/EMBL/DDBJ: DAA08374.1 \| X72701 \| Z73071
External Classifications	EC: 2.8.1.6 [Biotin synthase]

Amino Acid Sequence (or in FASTA format)
1 MMSTIYRHLS TARPALTKYA TNAAVKSTTA SSEASTLGAL QYALSLDEPS 51 HSWTKSQLKE IYHTPLLELT HAAQLQHRKW HDPTKVQLCT LMNIKSGGCS 101 EDCKYCAQSS RNDTGLKAEK MVKVDEVIKE AEEAKRNGST RFCLGAAWRD 151 MKGRKSAMKR IQEMVTKVND MGLETCVTLG MVDQDQAKQL KDAGLTAYNH 201 NIDTSREHYS KVITTRTYDD RLQTIKNVQE SGIKACTGGI LGLGESEDDH 251 IGFIYTLSNM SPHPESLPIN RLVAIKGTPM AEELADPKSK KLQFDEILRT 301 IATARIVMPK AIIRLAAGRY TMKETEQFVC FMAGCNSIFT GKKMLTTMCN 351 GWDEDKAMLA KWGLQPMEAF KYDRS*

Amino Acid Sequence (or in FASTA format)

       1  MMSTIYRHLS TARPALTKYA TNAAVKSTTA SSEASTLGAL QYALSLDEPS
      51  HSWTKSQLKE IYHTPLLELT HAAQLQHRKW HDPTKVQLCT LMNIKSGGCS
     101  EDCKYCAQSS RNDTGLKAEK MVKVDEVIKE AEEAKRNGST RFCLGAAWRD
     151  MKGRKSAMKR IQEMVTKVND MGLETCVTLG MVDQDQAKQL KDAGLTAYNH
     201  NIDTSREHYS KVITTRTYDD RLQTIKNVQE SGIKACTGGI LGLGESEDDH
     251  IGFIYTLSNM SPHPESLPIN RLVAIKGTPM AEELADPKSK KLQFDEILRT
     301  IATARIVMPK AIIRLAAGRY TMKETEQFVC FMAGCNSIFT GKKMLTTMCN
     351  GWDEDKAMLA KWGLQPMEAF KYDRS*

external links for Bio2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	GeneMANIA
YOGY

References cited on this page View Complete Literature Guide for Bio2p

1)	Zhang S, et al. (1994) The gene for biotin synthase from Saccharomyces cerevisiae: cloning, sequencing, and complementation of Escherichia coli strains lacking biotin synthase. Arch Biochem Biophys 309(1):29-35
2)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41