MES1/YGR264C Protein Information Help

Standard Name Mes1p
Systematic Name Ygr264cp
ORF Classification Verified
Description Methionyl-tRNA synthetase, forms a complex with glutamyl-tRNA synthetase (Gus1p) and Arc1p, which increases the catalytic efficiency of both tRNA synthetases; also has a role in nuclear export of tRNAs (1, 2, 3, 4) Also known as: MESI
Name Description MEthionyl-tRNA Synthetase
Gene Product MetRS 5 , methionyl-tRNA synthetase 1
Experimental Data
Molecules/cell 85000 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 751
Molecular Weight (Da) 85,677
Isoelectric Point (pI) 6.59

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Mes1p (InterPro)
Physical Interactions There are 40 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000053133 | P00958
MIPS: YGR264C
NCBI: 114794501 | 1323481 | 1536956 | 1711639 | 3924 | 398366347 | NP_011780.3
GenBank/EMBL/DDBJ: DAA08354.1 | V01316 | Y07777 | Z73049
External Classifications EC: 6.1.1.10 [Methionine--tRNA ligase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSFLISFDKS KKHPAHLQLA NNLKIALALE YASKNLKPEV DNDNAAMELR
      51  NTKEPFLLFD ANAILRYVMD DFEGQTSDKY QFALASLQNL LYHKELPQQH
     101  VEVLTNKAIE NYLVELKEPL TTTDLILFAN VYALNSSLVH SKFPELPSKV
     151  HNAVALAKKH VPRDSSSFKN IGAVKIQADL TVKPKDSEIL PKPNERNILI
     201  TSALPYVNNV PHLGNIIGSV LSADIFARYC KGRNYNALFI CGTDEYGTAT
     251  ETKALEEGVT PRQLCDKYHK IHSDVYKWFQ IGFDYFGRTT TDKQTEIAQH
     301  IFTKLNSNGY LEEQSMKQLY CPVHNSYLAD RYVEGECPKC HYDDARGDQC
     351  DKCGALLDPF ELINPRCKLD DASPEPKYSD HIFLSLDKLE SQISEWVEKA
     401  SEEGNWSKNS KTITQSWLKD GLKPRCITRD LVWGTPVPLE KYKDKVLYVW
     451  FDATIGYVSI TSNYTKEWKQ WWNNPEHVSL YQFMGKDNVP FHTVVFPGSQ
     501  LGTEENWTML HHLNTTEYLQ YENGKFSKSR GVGVFGNNAQ DSGISPSVWR
     551  YYLASVRPES SDSHFSWDDF VARNNSELLA NLGNFVNRLI KFVNAKYNGV
     601  VPKFDPKKVS NYDGLVKDIN EILSNYVKEM ELGHERRGLE IAMSLSARGN
     651  QFLQENKLDN TLFSQSPEKS DAVVAVGLNI IYAVSSIITP YMPEIGEKIN
     701  KMLNAPALKI DDRFHLAILE GHNINKAEYL FQRIDEKKID EWRAKYGGQQ
     751  V*

external links for Mes1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
Candida (CGD) BioPIXIE MIPS YeastGFP
Candida (CandidaDB) CYC2008 (complexes) Pfam domains YeastRC Public Image Repository
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Mes1p
1) Chatton B, et al.  (1987) Cloning and characterization of the yeast methionyl-tRNA synthetase mutation mes1. J Biol Chem 262(31):15094-7
2) Galani K, et al.  (2001) The intracellular location of two aminoacyl-tRNA synthetases depends on complex formation with Arc1p. EMBO J 20(23):6889-98
3) Feng W and Hopper AK  (2002) A Los1p-independent pathway for nuclear export of intronless tRNAs in Saccharomycescerevisiae. Proc Natl Acad Sci U S A 99(8):5412-7
4) Deinert K, et al.  (2001) Arc1p organizes the yeast aminoacyl-tRNA synthetase complex and stabilizes its interaction with the cognate tRNAs. J Biol Chem 276(8):6000-8
5) Simos G, et al.  (1996) The yeast protein Arc1p binds to tRNA and functions as a cofactor for the methionyl- and glutamyl-tRNA synthetases. EMBO J 15(19):5437-48
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41