BUD32/YGR262C Protein Information

Standard Name	Bud32p 1
Systematic Name	Ygr262cp
Alias	Ldb14p
ORF Classification	Verified
Description	Protein kinase, component of the EKC/KEOPS complex with Kae1p, Cgi121p, Pcc1p, and Gon7p; EKC/KEOPS complex is required for t6A tRNA modification and may have roles in telomere maintenance and transcription (1, 2, 3, 4, 5)
Name Description	BUD site selection 1

Experimental Data
Molecules/cell	3020 6

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	261
Molecular Weight (Da)	29,936
Isoelectric Point (pI)	9.15

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Bud32p (InterPro)
Physical Interactions	There are 71 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000126B2C \| P53323 MIPS: YGR262C NCBI: 1323477 \| 1536954 \| 1723770 \| 45270984 \| 6321701 \| NP_011778.1 GenBank/EMBL/DDBJ: DAA08352.1 \| AY558547 \| Y07777 \| Z73047
External Classifications	EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]

Amino Acid Sequence (or in FASTA format)
1 MTQEFIDKVS SYLTPDVDIA PISQGAEAIV FTTTTHPYLP RAKDSHQKYI 51 IKYRPPKRYR HPQIDQALTK HRTLNESRLL AKLYLIPGLC VPQLIACDPY 101 NGFIWLEFLG EDLPGGHGFS NLKNFLWMHD QDPYSDLVAT TLRKVGRQIG 151 LLHWNDYCHG DLTSSNIVLV RDGARWTPHL IDFGLGSVSN LVEDKGVDLY 201 VLERAILSTH SKHAEKYNAW IMEGFEEVYR EQGAKGAKKL KEVTKRFEEV 251 RLRGRKRSML G*

Amino Acid Sequence (or in FASTA format)

       1  MTQEFIDKVS SYLTPDVDIA PISQGAEAIV FTTTTHPYLP RAKDSHQKYI
      51  IKYRPPKRYR HPQIDQALTK HRTLNESRLL AKLYLIPGLC VPQLIACDPY
     101  NGFIWLEFLG EDLPGGHGFS NLKNFLWMHD QDPYSDLVAT TLRKVGRQIG
     151  LLHWNDYCHG DLTSSNIVLV RDGARWTPHL IDFGLGSVSN LVEDKGVDLY
     201  VLERAILSTH SKHAEKYNAW IMEGFEEVYR EQGAKGAKKL KEVTKRFEEV
     251  RLRGRKRSML G*

external links for Bud32p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Bud32p

1)	Ni L and Snyder M (2001) A genomic study of the bipolar bud site selection pattern in Saccharomyces cerevisiae. Mol Biol Cell 12(7):2147-70
2)	Stocchetto S, et al. (1997) Biochemical evidence that Saccharomyces cerevisiae YGR262c gene, required for normal growth, encodes a novel Ser/Thr-specific protein kinase. FEBS Lett 414(1):171-5
3)	Downey M, et al. (2006) A genome-wide screen identifies the evolutionarily conserved KEOPS complex as a telomere regulator. Cell 124(6):1155-68
4)	Kisseleva-Romanova E, et al. (2006) Yeast homolog of a cancer-testis antigen defines a new transcription complex. EMBO J 25(15):3576-85
5)	Srinivasan M, et al. (2011) The highly conserved KEOPS/EKC complex is essential for a universal tRNA modification, t6A. EMBO J 30(5):873-81
6)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41