BRF1/YGR246C Protein Information Help

Standard Name Brf1p 1
Systematic Name Ygr246cp
Alias Pcf4p , Tds4p
ORF Classification Verified
Description TFIIIB B-related factor, one of three subunits of RNA polymerase III transcription initiation factor TFIIIB, binds TFIIIC and TBP and recruits RNA pol III to promoters, amino-terminal half is homologous to TFIIB (2, 3, 4)
Name Description B-Related Factor 3
Gene Product TFIIIB70 5
Experimental Data
Molecules/cell 4330 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 596
Molecular Weight (Da) 66,906
Isoelectric Point (pI) 6.91

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Brf1p (InterPro)
Physical Interactions There are 39 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000136C57 | P29056
MIPS: YGR246C
NCBI: 1323446 | 135646 | 1702956 | 172897 | 172905 | 29726665 | 29726669 | 29726673 | 29726677 | 6321685 | NP_011762.1
GenBank/EMBL/DDBJ: DAA08337.1 | L00630 | M91073 | Y07703 | Z73031
Amino Acid Sequence (or in FASTA format)
 
       1  MPVCKNCHGT EFERDLSNAN NDLVCKACGV VSEDNPIVSE VTFGETSAGA
      51  AVVQGSFIGA GQSHAAFGGS SALESREATL NNARRKLRAV SYALHIPEYI
     101  TDAAFQWYKL ALANNFVQGR RSQNVIASCL YVACRKEKTH HMLIDFSSRL
     151  QVSVYSIGAT FLKMVKKLHI TELPLADPSL FIQHFAEKLD LADKKIKVVK
     201  DAVKLAQRMS KDWMFEGRRP AGIAGACILL ACRMNNLRRT HTEIVAVSHV
     251  AEETLQQRLN EFKNTKAAKL SVQKFRENDV EDGEARPPSF VKNRKKERKI
     301  KDSLDKEEMF QTSEEALNKN PILTQVLGEQ ELSSKEVLFY LKQFSERRAR
     351  VVERIKATNG IDGENIYHEG SENETRKRKL SEVSIQNEHV EGEDKETEGT
     401  EEKVKKVKTK TSEEKKENES GHFQDAIDGY SLETDPYCPR NLHLLPTTDT
     451  YLSKVSDDPD NLEDVDDEEL NAHLLNEEAS KLKERIWIGL NADFLLEQES
     501  KRLKQEADIA TGNTSVKKKR TRRRNNTRSD EPTKTVDAAA AIGLMSDLQD
     551  KSGLHAALKA AEESGDFTTA DSVKNMLQKA SFSKKINYDA IDGLFR*

external links for Brf1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Brf1p
1) Colbert T and Hahn S  (1992) A yeast TFIIB-related factor involved in RNA polymerase III transcription. Genes Dev 6(10):1940-9
2) Kassavetis GA, et al.  (1997) Domains of the Brf component of RNA polymerase III transcription factor IIIB (TFIIIB): functions in assembly of TFIIIB-DNA complexes and recruitment of RNA polymerase to the promoter. Mol Cell Biol 17(9):5299-306
3) Khoo B, et al.  (1994) Conserved functional domains of the RNA polymerase III general transcription factor BRF. Genes Dev 8(23):2879-90
4) Kassavetis GA, et al.  (1998) Functional and structural organization of Brf, the TFIIB-related component of the RNA polymerase III transcription initiation complex. Mol Cell Biol 18(9):5587-99
5) Andrau JC, et al.  (1999) Mutagenesis of yeast TFIIIB70 reveals C-terminal residues critical for interaction with TBP and C34. J Mol Biol 288(4):511-20
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41