YHB1/YGR234W Protein Information

Standard Name	Yhb1p
Systematic Name	Ygr234wp
Alias	Yhb4p
ORF Classification	Verified
Description	Nitric oxide oxidoreductase; flavohemoglobin involved in nitric oxide detoxification; plays a role in the oxidative and nitrosative stress responses; protein increases in abundance and relocalizes from nucleus to cytoplasmic foci upon DNA replication stress (1, 2, 3, 4)
Name Description	Yeast HemogloBin-like protein

Experimental Data
Molecules/cell	13000 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	399
Molecular Weight (Da)	44,646
Isoelectric Point (pI)	6.25

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Yhb1p (InterPro)
Physical Interactions	There are 56 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052ED7 \| P39676 MIPS: YGR234W NCBI: 13183165 \| 1323423 \| 51013379 \| 6321673 \| 729488 \| 886928 \| 88984161 \| NP_011750.1 GenBank/EMBL/DDBJ: DAA08325.1 \| AF239759 \| AY692964 \| L07070 \| L07071 \| X87941 \| Z73019
External Classifications	EC: 1.14.12.17 [Nitric oxide dioxygenase]

Amino Acid Sequence (or in FASTA format)
1 MLAEKTRSII KATVPVLEQQ GTVITRTFYK NMLTEHTELL NIFNRTNQKV 51 GAQPNALATT VLAAAKNIDD LSVLMDHVKQ IGHKHRALQI KPEHYPIVGE 101 YLLKAIKEVL GDAATPEIIN AWGEAYQAIA DIFITVEKKM YEEALWPGWK 151 PFDITAKEYV ASDIVEFTVK PKFGSGIELE SLPITPGQYI TVNTHPIRQE 201 NQYDALRHYS LCSASTKNGL RFAVKMEAAR ENFPAGLVSE YLHKDAKVGD 251 EIKLSAPAGD FAINKELIHQ NEVPLVLLSS GVGVTPLLAM LEEQVKCNPN 301 RPIYWIQSSY DEKTQAFKKH VDELLAECAN VDKIIVHTDT EPLINAAFLK 351 EKSPAHADVY TCGSLAFMQA MIGHLKELEH RDDMIHYEPF GPKMSTVQV*

Amino Acid Sequence (or in FASTA format)

       1  MLAEKTRSII KATVPVLEQQ GTVITRTFYK NMLTEHTELL NIFNRTNQKV
      51  GAQPNALATT VLAAAKNIDD LSVLMDHVKQ IGHKHRALQI KPEHYPIVGE
     101  YLLKAIKEVL GDAATPEIIN AWGEAYQAIA DIFITVEKKM YEEALWPGWK
     151  PFDITAKEYV ASDIVEFTVK PKFGSGIELE SLPITPGQYI TVNTHPIRQE
     201  NQYDALRHYS LCSASTKNGL RFAVKMEAAR ENFPAGLVSE YLHKDAKVGD
     251  EIKLSAPAGD FAINKELIHQ NEVPLVLLSS GVGVTPLLAM LEEQVKCNPN
     301  RPIYWIQSSY DEKTQAFKKH VDELLAECAN VDKIIVHTDT EPLINAAFLK
     351  EKSPAHADVY TCGSLAFMQA MIGHLKELEH RDDMIHYEPF GPKMSTVQV*

external links for Yhb1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
YGOB	CYC2008 (complexes)	Pfam domains
YOGY	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Yhb1p

1)	Zhu H and Riggs AF (1992) Yeast flavohemoglobin is an ancient protein related to globins and a reductase family. Proc Natl Acad Sci U S A 89(11):5015-9
2)	Liu L, et al. (2000) Protection from nitrosative stress by yeast flavohemoglobin. Proc Natl Acad Sci U S A 97(9):4672-6
3)	Cassanova N, et al. (2005) Yeast flavohemoglobin, a nitric oxide oxidoreductase, is located in both the cytosol and the mitochondrial matrix: effects of respiration, anoxia, and the mitochondrial genome on its intracellular level and distribution. J Biol Chem 280(9):7645-53
4)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41