PCT1/YGR202C Protein Information Help

Standard Name Pct1p
Systematic Name Ygr202cp
Alias Bsr2p , Cct1p 1
ORF Classification Verified
Description Cholinephosphate cytidylyltransferase; a rate-determining enzyme of the CDP-choline pathway for phosphatidylcholine synthesis, inhibited by Sec14p, activated upon lipid-binding; contains an element within the regulatory domain involved in both silencing and activation of enzymatic activity (1, 2, 3, 4, 5, 6)
Name Description Phosphocholine CytidylylTransferase 7
Experimental Data
Molecules/cell 3050 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 424
Molecular Weight (Da) 49,406
Isoelectric Point (pI) 9.26

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Pct1p (InterPro)
Physical Interactions There are 10 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000168338 | P13259
MIPS: YGR202C
NCBI: 1323361 | 171179 | 45269571 | 52788237 | 6321641 | 790493 | NP_011718.1 | NM_001181331.1
GenBank/EMBL/DDBJ: DAA08295.1 | AY557840 | M36827 | Z49133 | Z72987
External Classifications EC: 2.7.7.15 [Choline-phosphate cytidylyltransferase]
Amino Acid Sequence (or in FASTA format)
 
       1  MANPTTGKSS IRAKLSNSSL SNLFKKNKNK RQREETEEQD NEDKDESKNQ
      51  DENKDTQLTP RKRRRLTKEF EEKEARYTNE LPKELRKYRP KGFRFNLPPT
     101  DRPIRIYADG VFDLFHLGHM KQLEQCKKAF PNVTLIVGVP SDKITHKLKG
     151  LTVLTDKQRC ETLTHCRWVD EVVPNAPWCV TPEFLLEHKI DYVAHDDIPY
     201  VSADSDDIYK PIKEMGKFLT TQRTNGVSTS DIITKIIRDY DKYLMRNFAR
     251  GATRQELNVS WLKKNELEFK KHINEFRSYF KKNQTNLNNA SRDLYFEVRE
     301  ILLKKTLGKK LYSKLIGNEL KKQNQRQRKQ NFLDDPFTRK LIREASPATE
     351  FANEFTGENS TAKSPDDNGN LFSQEDDEDT NSNNTNTNSD SDSNTNSTPP
     401  SEDDDDNDRL TLENLTQKKK QSAN*

external links for Pct1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Pct1p
1) Tsukagoshi Y, et al.  (1987) Molecular cloning and characterization of the gene encoding cholinephosphate cytidylyltransferase in Saccharomyces cerevisiae. Eur J Biochem 169(3):477-86
2) Skinner HB, et al.  (1995) The Saccharomyces cerevisiae phosphatidylinositol-transfer protein effects a ligand-dependent inhibition of choline-phosphate cytidylyltransferase activity. Proc Natl Acad Sci U S A 92(1):112-6
3) Sreenivas A, et al.  (1998) A role for phospholipase D (Pld1p) in growth, secretion, and regulation of membrane lipid synthesis in yeast. J Biol Chem 273(27):16635-8
4) Dowd SR, et al.  (2001) Turnover of phosphatidylcholine in Saccharomyces cerevisiae. The role of the CDP-choline pathway. J Biol Chem 276(6):3756-63
5) Friesen JA, et al.  (2001) Purification and kinetic characterization of CTP:phosphocholine cytidylyltransferase from Saccharomyces cerevisiae. Protein Expr Purif 21(1):141-8
6) Ding Z, et al.  (2012) A 22-mer segment in the structurally pliable regulatory domain of metazoan CTP: phosphocholine cytidylyltransferase facilitates both silencing and activating functions. J Biol Chem 287(46):38980-91
7) Johnson JE, et al.  (1992) Comparison of the lipid regulation of yeast and rat CTP: phosphocholine cytidylyltransferase expressed in COS cells. Biochem J 285 ( Pt 3)():815-20
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41