TDH3/YGR192C Protein Information

Standard Name	Tdh3p
Systematic Name	Ygr192cp
Alias	Gld1p , Hsp35p , Hsp36p , Sss2p
ORF Classification	Verified
Description	Glyceraldehyde-3-phosphate dehydrogenase, isozyme 3; involved in glycolysis and gluconeogenesis; tetramer that catalyzes the reaction of glyceraldehyde-3-phosphate to 1,3 bis-phosphoglycerate; detected in the cytoplasm and cell wall; TDH3 has a paralog, TDH2, that arose from the whole genome duplication (1, 2, 3)
Name Description	Triose-phosphate DeHydrogenase 2
Gene Product	GAPDH 4 , GPD

Experimental Data
Molecules/cell	169000 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	332
Molecular Weight (Da)	35,746
Isoelectric Point (pI)	6.96

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tdh3p (InterPro)
Signal Peptide(s)	There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions	There are 120 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004F91A \| P00359 MIPS: YGR192C NCBI: 1169787 \| 1323341 \| 171541 \| 371927386 \| 371927387 \| 3720 \| 398366083 \| 448262666 \| 45269553 \| 755797 \| NP_011708.3 GenBank/EMBL/DDBJ: DAA08285.1 \| AY557831 \| J01324 \| V01300 \| X82408 \| Z72977
External Classifications	EC: 1.2.1.12 [Glyceraldehyde-3-phosphate dehydrogenase (phosphorylating)]

Amino Acid Sequence (or in FASTA format)
1 MVRVAINGFG RIGRLVMRIA LSRPNVEVVA LNDPFITNDY AAYMFKYDST 51 HGRYAGEVSH DDKHIIVDGK KIATYQERDP ANLPWGSSNV DIAIDSTGVF 101 KELDTAQKHI DAGAKKVVIT APSSTAPMFV MGVNEEKYTS DLKIVSNASC 151 TTNCLAPLAK VINDAFGIEE GLMTTVHSLT ATQKTVDGPS HKDWRGGRTA 201 SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLNKE 251 TTYDEIKKVV KAAAEGKLKG VLGYTEDAVV SSDFLGDSHS SIFDASAGIQ 301 LSPKFVKLVS WYDNEYGYST RVVDLVEHVA KA*

Amino Acid Sequence (or in FASTA format)

       1  MVRVAINGFG RIGRLVMRIA LSRPNVEVVA LNDPFITNDY AAYMFKYDST
      51  HGRYAGEVSH DDKHIIVDGK KIATYQERDP ANLPWGSSNV DIAIDSTGVF
     101  KELDTAQKHI DAGAKKVVIT APSSTAPMFV MGVNEEKYTS DLKIVSNASC
     151  TTNCLAPLAK VINDAFGIEE GLMTTVHSLT ATQKTVDGPS HKDWRGGRTA
     201  SGNIIPSSTG AAKAVGKVLP ELQGKLTGMA FRVPTVDVSV VDLTVKLNKE
     251  TTYDEIKKVV KAAAEGKLKG VLGYTEDAVV SSDFLGDSHS SIFDASAGIQ
     301  LSPKFVKLVS WYDNEYGYST RVVDLVEHVA KA*

external links for Tdh3p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Tdh3p

1)	McAlister L and Holland MJ (1985) Differential expression of the three yeast glyceraldehyde-3-phosphate dehydrogenase genes. J Biol Chem 260(28):15019-27
2)	McAlister L and Holland MJ (1985) Isolation and characterization of yeast strains carrying mutations in the glyceraldehyde-3-phosphate dehydrogenase genes. J Biol Chem 260(28):15013-8
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Delgado ML, et al. (2001) The glyceraldehyde-3-phosphate dehydrogenase polypeptides encoded by the Saccharomyces cerevisiae TDH1, TDH2 and TDH3 genes are also cell wall proteins. Microbiology 147(Pt 2):411-7
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41