CRH1/YGR189C Protein Information Help

Standard Name Crh1p 1
Systematic Name Ygr189cp
ORF Classification Verified
Description Chitin transglycosylase that functions in the transfer of chitin to beta(1-6) and beta(1-3) glucans in the cell wall; similar and functionally redundant to Utr2; localizes to sites of polarized growth; expression induced by cell wall stress (1, 2, 3, 4, 5)
Name Description Congo Red Hypersensitive 1
Experimental Data
Molecules/cell 29500 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 507
Molecular Weight (Da) 52,757
Isoelectric Point (pI) 4.3

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Crh1p (InterPro)
Signal Peptide(s) There are 1 total predicted predicted signal peptide(s) (SignalP)
Physical Interactions There are 4 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013AF95 | P53301
MIPS: YGR189C
NCBI: 1323336 | 1430955 | 1723734 | 6321628 | NP_011705.1
GenBank/EMBL/DDBJ: DAA08283.1 | X99074 | Z72974
External Classifications EC: 3.2.-.- [Glycosylases]
Amino Acid Sequence (or in FASTA format)
 
       1  MKVLDLLTVL SASSLLSTFA AAESTATADS TTAASSTASC NPLKTTGCTP
      51  DTALATSFSE DFSSSSKWFT DLKHAGEIKY GSDGLSMTLA KRYDNPSLKS
     101  NFYIMYGKLE VILKAANGTG IVSSFYLQSD DLDEIDIEWV GGDNTQFQSN
     151  FFSKGDTTTY DRGEFHGVDT PTDKFHNYTL DWAMDKTTWY LDGESVRVLS
     201  NTSSEGYPQS PMYLMMGIWA GGDPDNAAGT IEWAGGETNY NDAPFTMYIE
     251  KVIVTDYSTG KKYTYGDQSG SWESIEADGG SIYGRYDQAQ EDFAVLANGG
     301  SISSSSTSSS TVSSSASSTV SSSVSSTVSS SASSTVSSSV SSTVSSSSSV
     351  SSSSSTSPSS STATSSKTLA SSSVTTSSSI SSFEKQSSSS SKKTVASSST
     401  SESIISSTKT PATVSSTTRS TVAPTTQQSS VSSDSPVQDK GGVATSSNDV
     451  TSSTTQISSK YTSTIQSSSS EASSTNSVQI SNGADLAQSL PREGKLFSVL
     501  VALLALL*

external links for Crh1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Crh1p
1) Rodriguez-Pena JM, et al.  (2000) A novel family of cell wall-related proteins regulated differently during the yeast life cycle. Mol Cell Biol 20(9):3245-55
2) Cabib E, et al.  (2007) Crh1p and Crh2p are required for the cross-linking of chitin to beta(1-6)glucan in the Saccharomyces cerevisiae cell wall. Mol Microbiol 63(3):921-35
3) Bermejo C, et al.  (2008) The Sequential Activation of the Yeast HOG and SLT2 Pathways Is Required for Cell Survival to Cell Wall Stress. Mol Biol Cell 19(3):1113-24
4) Cabib E, et al.  (2008) Assembly of the Yeast Cell Wall: Crh1p AND Crh2p ACT AS TRANSGLYCOSYLASES IN VIVO AND IN VITRO. J Biol Chem 283(44):29859-72
5) Cabib E  (2009) Two novel techniques for determination of polysaccharide cross-links show that Crh1p and Crh2p attach chitin to both beta(1-6)- and beta(1-3)glucan in the Saccharomyces cerevisiae cell wall. Eukaryot Cell 8(11):1626-36
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41