TFG1/YGR186W Protein Information Help

Standard Name Tfg1p 1
Systematic Name Ygr186wp
Alias Ssu71p 2 , Rap74p
ORF Classification Verified
Description TFIIF (Transcription Factor II) largest subunit; involved in both transcription initiation and elongation of RNA polymerase II; homologous to human RAP74 (2, 3)
Name Description Transcription Factor G 1
Experimental Data
Molecules/cell 1920 4
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 735
Molecular Weight (Da) 82,194
Isoelectric Point (pI) 5.48

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Tfg1p (InterPro)
Physical Interactions There are 131 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000005319A | P41895
MIPS: YGR186W
NCBI: 1323330 | 1430952 | 1729816 | 606955 | 6321625 | 639700 | NP_011702.1 | NM_001181315.1
GenBank/EMBL/DDBJ: DAA08280.1 | U13015 | U14527 | X99074 | Z72971
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MSRRNPPGSR NGGGPTNASP FIKRDRMRRN FLRMRMGQNG SNSSSPGVPN
      51  GDNSRGSLVK KDDPEYAEER EKMLLQIGVE ADAGRSNVKV KDEDPNEYNE
     101  FPLRAIPKED LENMRTHLLK FQSKKKINPV TDFHLPVRLH RKDTRNLQFQ
     151  LTRAEIVQRQ KEISEYKKKA EQERSTPNSG GMNKSGTVSL NNTVKDGSQT
     201  PTVDSVTKDN TANGVNSSIP TVTGSSVPPA SPTTVSAVES NGLSNGSTSA
     251  ANGLDGNAST ANLANGRPLV TKLEDAGPAE DPTKVGMVKY DGKEVTNEPE
     301  FEEGTMDPLA DVAPDGGGRA KRGNLRRKTR QLKVLDENAK KLRFEEFYPW
     351  VMEDFDGYNT WVGSYEAGNS DSYVLLSVED DGSFTMIPAD KVYKFTARNK
     401  YATLTIDEAE KRMDKKSGEV PRWLMKHLDN IGTTTTRYDR TRRKLKAVAD
     451  QQAMDEDDRD DNSEVELDYD EEFADDEEAP IIDGNEQENK ESEQRIKKEM
     501  LQANAMGLRD EEAPSENEED ELFGEKKIDE DGERIKKALQ KTELAALYSS
     551  DENEINPYLS ESDIENKENE SPVKKEEDSD TLSKSKRSSP KKQQKKATNA
     601  HVHKEPTLRV KSIKNCVIIL KGDKKILKSF PEGEWNPQTT KAVDSSNNAS
     651  NTVPSPIKQE EGLNSTVAER EETPAPTITE KDIIEAIGDG KVNIKEFGKF
     701  IRRKYPGAEN KKLMFAIVKK LCRKVGNDHM ELKKE*

external links for Tfg1p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Tfg1p
1) Henry NL, et al.  (1994) TFIIF-TAF-RNA polymerase II connection. Genes Dev 8(23):2868-78
2) Sun ZW and Hampsey M  (1995) Identification of the gene (SSU71/TFG1) encoding the largest subunit of transcription factor TFIIF as a suppressor of a TFIIB mutation in Saccharomyces cerevisiae. Proc Natl Acad Sci U S A 92(8):3127-31
3) Hampsey M  (1998) Molecular genetics of the RNA polymerase II general transcriptional machinery. Microbiol Mol Biol Rev 62(2):465-503
4) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41