MSM1/YGR171C Protein Information

Standard Name	Msm1p
Systematic Name	Ygr171cp
ORF Classification	Verified
Description	Mitochondrial methionyl-tRNA synthetase (MetRS), functions as a monomer in mitochondrial protein synthesis; functions similarly to cytoplasmic MetRS although the cytoplasmic form contains a zinc-binding domain not found in Msm1p (1, 2, 3)
Name Description	Mitochondrial aminoacyl-tRNA Synthetase, Methionine 1
Gene Product	mitochondrial methionyl-tRNA synthetase 1

Experimental Data
Molecules/cell	3120 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	575
Molecular Weight (Da)	66,734
Isoelectric Point (pI)	9.27

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Msm1p (InterPro)
Physical Interactions	There are 3 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000136580 \| P22438 MIPS: YGR171C NCBI: 1323302 \| 1711640 \| 3998 \| 6321610 \| NP_011687.1 GenBank/EMBL/DDBJ: DAA08266.1 \| X14629 \| Z72956
External Classifications	EC: 6.1.1.10 [Methionine--tRNA ligase]

Amino Acid Sequence (or in FASTA format)
1 MQCRSIVHRL YSKVSHVTTP IFYPNAKPHL GHLYSSLLSD VYHRWQLFKG 51 NLSFFTTGTD EHGLKIQCAS ESNGFDQPKK FVDKLYPEFV QLDKIYGINY 101 TRFIRTTDPD HIENVMKLWE LCLKNGYIYM GEHKGWYSIS DETFYPESKV 151 IKDPKNDGKY LNTESKNEVV YQSETNYFFR LSLFNKKIVD HIRKNPDFIF 201 PASKRDQILK ELETGGTLPD LSISRPSARL KWGIPTPNDP SQKVYVWFDA 251 LCNYLSSIGG IPSILSNATE VVSRHYSDKS NVKGQLLIPY PKEVQRNTIH 301 VIGKDIAKFH TVYWPSFLLA AGLPLPRQIV VHGHWLCNGM KMSKSLGNVV 351 DPIDMARYYG ADIVRWFLLE NSKLEEDGDF QEAKLYETRE LLVSKWGNLI 401 NRCCGSKFNI ERAVMKFSDK ANFQFQEIFQ NEPIVSERIE NLAKLLNKSQ 451 EVFDEKIAIF QYPQLLRHVW SIINDANTLV QNSKPWEREL DQQDNIIFLA 501 METSRILSIL CQSIIPSLSQ SFLDRIDVSK EKRTINYARL GSDKTYGKQS 551 NKKGREVPLK KIPFRLQEEQ TNMRS*

Amino Acid Sequence (or in FASTA format)

       1  MQCRSIVHRL YSKVSHVTTP IFYPNAKPHL GHLYSSLLSD VYHRWQLFKG
      51  NLSFFTTGTD EHGLKIQCAS ESNGFDQPKK FVDKLYPEFV QLDKIYGINY
     101  TRFIRTTDPD HIENVMKLWE LCLKNGYIYM GEHKGWYSIS DETFYPESKV
     151  IKDPKNDGKY LNTESKNEVV YQSETNYFFR LSLFNKKIVD HIRKNPDFIF
     201  PASKRDQILK ELETGGTLPD LSISRPSARL KWGIPTPNDP SQKVYVWFDA
     251  LCNYLSSIGG IPSILSNATE VVSRHYSDKS NVKGQLLIPY PKEVQRNTIH
     301  VIGKDIAKFH TVYWPSFLLA AGLPLPRQIV VHGHWLCNGM KMSKSLGNVV
     351  DPIDMARYYG ADIVRWFLLE NSKLEEDGDF QEAKLYETRE LLVSKWGNLI
     401  NRCCGSKFNI ERAVMKFSDK ANFQFQEIFQ NEPIVSERIE NLAKLLNKSQ
     451  EVFDEKIAIF QYPQLLRHVW SIINDANTLV QNSKPWEREL DQQDNIIFLA
     501  METSRILSIL CQSIIPSLSQ SFLDRIDVSK EKRTINYARL GSDKTYGKQS
     551  NKKGREVPLK KIPFRLQEEQ TNMRS*

external links for Msm1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Candida (CGD)	BioPIXIE	MIPS	YeastGFP
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Msm1p

1)	Tzagoloff A, et al. (1989) Characterization of MSM1, the structural gene for yeast mitochondrial methionyl-tRNA synthetase. Eur J Biochem 179(2):365-71
2)	Schwob E, et al. (1988) Purification of the yeast mitochondrial methionyl-tRNA synthetase. Common and distinctive features of the cytoplasmic and mitochondrial isoenzymes. Eur J Biochem 178(1):235-42
3)	Senger B, et al. (2001) Yeast cytoplasmic and mitochondrial methionyl-tRNA synthetases: two structural frameworks for identical functions. J Mol Biol 311(1):205-16
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41