PRE9/YGR135W Protein Information

Standard Name	Pre9p
Systematic Name	Ygr135wp
ORF Classification	Verified
Description	Alpha 3 subunit of the 20S proteasome, the only nonessential 20S subunit; may be replaced by the alpha 4 subunit (Pre6p) under stress conditions to create a more active proteasomal isoform (1, 2)
Name Description	PRoteinase yscE

Experimental Data
Molecules/cell	17100 3

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	258
Molecular Weight (Da)	28,714
Isoelectric Point (pI)	4.91

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Pre9p (InterPro)
Physical Interactions	There are 123 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000004F918 \| P23638 MIPS: YGR135W NCBI: 112490844 \| 112490858 \| 11513414 \| 11513428 \| 11513993 \| 11514007 \| 130875 \| 1323225 \| 14488807 \| 14488821 \| 172262 \| 178847461 \| 178847475 \| 178847503 \| 178847517 \| 190016368 \| 190016382 \| 197725322 \| 197725336 \| 20150378 \| 20150399 \| 211939213 \| 211939227 \| 211939241 \| 211939255 \| 238828238 \| 238828252 \| 258588322 \| 258588336 \| 258588350 \| 258588364 \| 258588544 \| 258588558 \| 298508224 \| 298508238 \| 298508440 \| 298508454 \| 3114271 \| 3114285 \| 323462937 \| 323462951 \| 323462965 \| 323462979 \| 323462993 \| 323463007 \| 330689382 \| 330689396 \| 333361145 \| 333361159 \| 333361173 \| 333361187 \| 333361201 \| 333361215 \| 333361229 \| 333361243 \| 335892342 \| 335892356 \| 340780420 \| 340780434 \| 340780448 \| 340780462 \| 350610673 \| 350610687 \| 359545847 \| 359545861 \| 378792242 \| 378792256 \| 378792276 \| 378792290 \| 390980817 \| 390980831 \| 390980845 \| 390980859 \| 398365831 \| 402550733 \| 402550747 \| 403071963 \| 403072193 \| 409973861 \| 409973875 \| 409973889 \| 409973903 \| 444302340 \| 444302354 \| 444302368 \| 444302382 \| 444302396 \| 444302410 \| 506480 \| 75765701 \| 75765715 \| 93279369 \| 93279383 \| 99032203 \| 99032217 \| NP_011651.3 \| NM_001181264.3 GenBank/EMBL/DDBJ: DAA08228.1 \| M63851 \| X56730 \| Z72920
External Classifications	EC: 3.4.25.1 [Proteasome endopeptidase complex]

Amino Acid Sequence (or in FASTA format)
1 MGSRRYDSRT TIFSPEGRLY QVEYALESIS HAGTAIGIMA SDGIVLAAER 51 KVTSTLLEQD TSTEKLYKLN DKIAVAVAGL TADAEILINT ARIHAQNYLK 101 TYNEDIPVEI LVRRLSDIKQ GYTQHGGLRP FGVSFIYAGY DDRYGYQLYT 151 SNPSGNYTGW KAISVGANTS AAQTLLQMDY KDDMKVDDAI ELALKTLSKT 201 TDSSALTYDR LEFATIRKGA NDGEVYQKIF KPQEIKDILV KTGITKKDED 251 EEADEDMK*

Amino Acid Sequence (or in FASTA format)

       1  MGSRRYDSRT TIFSPEGRLY QVEYALESIS HAGTAIGIMA SDGIVLAAER
      51  KVTSTLLEQD TSTEKLYKLN DKIAVAVAGL TADAEILINT ARIHAQNYLK
     101  TYNEDIPVEI LVRRLSDIKQ GYTQHGGLRP FGVSFIYAGY DDRYGYQLYT
     151  SNPSGNYTGW KAISVGANTS AAQTLLQMDY KDDMKVDDAI ELALKTLSKT
     201  TDSSALTYDR LEFATIRKGA NDGEVYQKIF KPQEIKDILV KTGITKKDED
     251  EEADEDMK*

external links for Pre9p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA
	YeastRC Two-Hybrid (Seattle)

References cited on this page View Complete Literature Guide for Pre9p

1)	Hochstrasser M (1996) Ubiquitin-dependent protein degradation. Annu Rev Genet 30:405-39
2)	Kusmierczyk AR, et al. (2008) A multimeric assembly factor controls the formation of alternative 20S proteasomes. Nat Struct Mol Biol 15(3):237-44
3)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41