ASN2/YGR124W Protein Information

Standard Name	Asn2p 1, 2
Systematic Name	Ygr124wp
ORF Classification	Verified
Description	Asparagine synthetase; catalyzes the synthesis of L-asparagine from L-aspartate in the asparagine biosynthetic pathway; ASN2 has a paralog, ASN1, that arose from the whole genome duplication (2, 3)
Name Description	ASparagiNe requiring 1

Experimental Data
Molecules/cell	58200 4

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	572
Molecular Weight (Da)	64,593
Isoelectric Point (pI)	5.73

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Asn2p (InterPro)
Physical Interactions	There are 17 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000168337 \| P49090 MIPS: YGR124W NCBI: 1323203 \| 1351986 \| 51013167 \| 6321563 \| 642347 \| NP_011640.1 GenBank/EMBL/DDBJ: DAA08217.1 \| AY692858 \| X83099 \| Z72909
External Classifications	EC: 6.3.5.4 [Asparagine synthase (glutamine-hydrolyzing)]

Amino Acid Sequence (or in FASTA format)
1 MCGIFAAFKH EDIHNFKPKA LQLSKKIRHR GPDWSGNAVM NSTIFVHERL 51 AIVGLDSGAQ PITSADGEYM LGVNGEIYNH IQLREMCSDY KFQTFSDCEP 101 IIPLYLEHDI DAPKYLDGMF AFCLYDSKKD RIVAARDPIG VVTLYMGRSS 151 QSPETVYFAS ELKCLTDVCD SIISFPPGHV YDSETDKITR YFTPDWLDEK 201 RIPSTPVDYH AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIAAIAARE 251 TEKANADANE DNNVDEKQLA GIDDQGHLHT SGWSRLHSFA IGLPNAPDLQ 301 AARKVAKFIG SIHHEHTFTL QEGLDALDDV IYHLETYDVT TIRASTPMFL 351 LSRKIKAQGV KMVLSGEGSD EIFGGYLYFA QAPSAAEFHT ESVQRVKNLH 401 LADCLRANKS TMAWGLEARV PFLDKDFLQL CMNIDPNEKM IKPKEGRIEK 451 YILRKAFDTT DEPDVKPYLP EEILWRQKEQ FSDGVGYSWI DGLRDTAERA 501 ISDAMFANPK ADWGDDIPTT KEAYWYRLKF DAWFPQKTAA DTVMRWIPKA 551 DWGCAEDPSG RYAKIHEKHV SA*

Amino Acid Sequence (or in FASTA format)

       1  MCGIFAAFKH EDIHNFKPKA LQLSKKIRHR GPDWSGNAVM NSTIFVHERL
      51  AIVGLDSGAQ PITSADGEYM LGVNGEIYNH IQLREMCSDY KFQTFSDCEP
     101  IIPLYLEHDI DAPKYLDGMF AFCLYDSKKD RIVAARDPIG VVTLYMGRSS
     151  QSPETVYFAS ELKCLTDVCD SIISFPPGHV YDSETDKITR YFTPDWLDEK
     201  RIPSTPVDYH AIRHSLEKAV RKRLMAEVPY GVLLSGGLDS SLIAAIAARE
     251  TEKANADANE DNNVDEKQLA GIDDQGHLHT SGWSRLHSFA IGLPNAPDLQ
     301  AARKVAKFIG SIHHEHTFTL QEGLDALDDV IYHLETYDVT TIRASTPMFL
     351  LSRKIKAQGV KMVLSGEGSD EIFGGYLYFA QAPSAAEFHT ESVQRVKNLH
     401  LADCLRANKS TMAWGLEARV PFLDKDFLQL CMNIDPNEKM IKPKEGRIEK
     451  YILRKAFDTT DEPDVKPYLP EEILWRQKEQ FSDGVGYSWI DGLRDTAERA
     501  ISDAMFANPK ADWGDDIPTT KEAYWYRLKF DAWFPQKTAA DTVMRWIPKA
     551  DWGCAEDPSG RYAKIHEKHV SA*

external links for Asn2p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	Organelle DB
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YPL+
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastGFP
Candida (CGD)	CYC2008 (complexes)	Pfam domains	YeastRC Public Image Repository
Candida (CandidaDB)	Complexome	YeastRC Structure Prediction (Seattle)
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Asn2p

1)	Jones GE (1978) L-Asparagine auxotrophs of Saccharomyces cerevisiae: genetic and phenotypic characterization. J Bacteriol 134(1):200-7
2)	Dang VD, et al. (1996) Cloning of the ASN1 and ASN2 genes encoding asparagine synthetases in Saccharomyces cerevisiae: differential regulation by the CCAAT-box-binding factor. Mol Microbiol 22(4):681-92
3)	Byrne KP and Wolfe KH (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
4)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41