SPT6/YGR116W Protein Information Help

Standard Name Spt6p 1
Systematic Name Ygr116wp
Alias Cre2p 2 , Ssn20p 3
ORF Classification Verified
Description Nucleosome remodeling protein; functions in various aspects of transcription, chromatin maintenance, and RNA processing; required for the maintenance of chromatin structure during transcription in order to inhibit transcription from promoters within the coding region (4, 5, 6, 7)
Name Description SuPpressor of Ty's 1
Experimental Data
Molecules/cell 8890 8
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 1,451
Molecular Weight (Da) 168,290
Isoelectric Point (pI) 4.73

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Spt6p (InterPro)
Physical Interactions There are 49 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000052E79 | P23615
MIPS: YGR116W
NCBI: 1323187 | 134854 | 172682 | 1945326 | 312597466 | 312597467 | 327200637 | 327200638 | 327200639 | 327200640 | 327200641 | 327200642 | 327200643 | 340707286 | 6321552 | NP_011631.1
GenBank/EMBL/DDBJ: DAA08208.1 | M34391 | Z72899 | Z72902
Amino Acid Sequence (or in FASTA format)
 
       1  MEETGDSKLV PRDEEEIVND NDETKAPSEE EEGEDVFDSS EEDEDIDEDE
      51  DEARKVQEGF IVNDDDENED PGTSISKKRR KHKRREREED DRLSEDDLDL
     101  LMENAGVERT KASSSSGKFK RLKRVGDEGN AAESESDNVA ASRQDSTSKL
     151  EDFFSEDEEE EESGLRNGRN NEYGRDEEDH ENRNRTADKG GILDELDDFI
     201  EDDEFSDEDD ETRQRRIQEK KLLREQSIKQ PTQITGLSSD KIDEMYDIFG
     251  DGHDYDWALE IENEELENGN DNNEAEEEEI DEETGAIKST KKKISLQDIY
     301  DLEDLKKNLM TEGDMKIRKT DIPERYQELR AGITDYGNMS SEDQELERNW
     351  IAEKISVDKN FDANYDLTEF KEAIGNAIKF ITKENLEVPF IYAYRRNYIS
     401  SREKDGFLLT EDDLWDIVSL DIEFHSLVNK KDYVQRFYAE LHIDDPIVTE
     451  YFKNQNTASI AELNSLQDIY DYLEFKYANE INEMFINHTG KTGKKHLKNS
     501  SYEKFKASPL YQAVSDIGIS AEDVGENISS QHQIHPPVDH PSSKPVEVIE
     551  SILNANSGDL QVFTSNTKLA IDTVQKYYSL ELSKNTKIRE KVRSDFSKYY
     601  LADVVLTAKG KKEIQKGSLY EDIKYAINRT PMHFRRDPDV FLKMVEAESL
     651  NLLSVKLHMS SQAQYIEHLF QIALETTNTS DIAIEWNNFR KLAFNQAMDK
     701  IFQDISQEVK DNLTKNCQKL VAKTVRHKFM TKLDQAPFIP NVRDPKIPKI
     751  LSLTCGQGRF GADAIIAVYV NRKGDFIRDY KIVDNPFDKT NPEKFEDTLD
     801  NIIQSCQPNA IGINGPNPKT QKFYKRLQEV LHKKQIVDSR GHTIPIIYVE
     851  DEVAIRYQNS ERAAQEFPNK PPLVKYCIAL ARYMHSPLLE YANLTSEEVR
     901  SLSIHPHQNL LSSEQLSWAL ETAFVDIVNL VSVEVNKATD NNYYASALKY
     951  ISGFGKRKAI DFLQSLQRLN EPLLARQQLI THNILHKTIF MNSAGFLYIS
    1001  WNEKRQKYED LEHDQLDSTR IHPEDYHLAT KVAADALEYD PDTIAEKEEQ
    1051  GTMSEFIELL REDPDRRAKL ESLNLESYAE ELEKNTGLRK LNNLNTIVLE
    1101  LLDGFEELRN DFHPLQGDEI FQSLTGESEK TFFKGSIIPV RVERFWHNDI
    1151  ICTTNSEVEC VVNAQRHAGA QLRRPANEIY EIGKTYPAKV IYIDYANITA
    1201  EVSLLDHDVK QQYVPISYSK DPSIWDLKQE LEDAEEERKL MMAEARAKRT
    1251  HRVINHPYYF PFNGRQAEDY LRSKERGEFV IRQSSRGDDH LVITWKLDKD
    1301  LFQHIDIQEL EKENPLALGK VLIVDNQKYN DLDQIIVEYL QNKVRLLNEM
    1351  TSSEKFKSGT KKDVVKFIED YSRVNPNKSV YYFSLNHDNP GWFYLMFKIN
    1401  ANSKLYTWNV KLTNTGYFLV NYNYPSVIQL CNGFKTLLKS NSSKNRMNNY
    1451  R*

external links for Spt6p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
Candida (CGD) BioPIXIE MIPS YeastRC Public Image Repository
Candida (CandidaDB) CYC2008 (complexes) Pfam domains
YGOB Complexome YeastRC Structure Prediction (Seattle)
YOGY DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Spt6p
1) Winston F, et al.  (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97
2) Denis CL  (1984) Identification of new genes involved in the regulation of yeast alcohol dehydrogenase II. Genetics 108(4):833-44
3) Neigeborn L, et al.  (1986) Suppressors of SNF2 mutations restore invertase derepression and cause temperature-sensitive lethality in yeast. Genetics 112(4):741-53
4) Bortvin A and Winston F  (1996) Evidence that Spt6p controls chromatin structure by a direct interaction with histones. Science 272(5267):1473-6
5) Hartzog GA, et al.  (1998) Evidence that Spt4, Spt5, and Spt6 control transcription elongation by RNA polymerase II in Saccharomyces cerevisiae. Genes Dev 12(3):357-69
6) Kaplan CD, et al.  (2003) Transcription elongation factors repress transcription initiation from cryptic sites. Science 301(5636):1096-9
7) Close D, et al.  (2011) Crystal Structures of the S. cerevisiae Spt6 Core and C-Terminal Tandem SH2 Domain. J Mol Biol 408(4):697-713
8) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41