DAM1/YGR113W Protein Information

Standard Name	Dam1p
Systematic Name	Ygr113wp
ORF Classification	Verified
Description	Essential subunit of the Dam1 complex (aka DASH complex), couples kinetochores to the force produced by MT depolymerization thereby aiding in chromosome segregation; Ipl1p target for regulating kinetochore-MT attachments (1, 2, 3, 4, 5, 6)
Name Description	Duo1 And Mps1 interacting

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	343
Molecular Weight (Da)	38,422
Isoelectric Point (pI)	9.97

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Dam1p (InterPro)
Physical Interactions	There are 105 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI0000052FE3 \| P53267 MIPS: YGR113W NCBI: 1323183 \| 398365747 \| 67476766 \| 8927583 \| NP_011628.4 GenBank/EMBL/DDBJ: DAA08207.1 \| AF280542 \| Z72898

Amino Acid Sequence (or in FASTA format)
1 MSEDKAKLGT TRSATEYRLS IGSAPTSRRS SMGESSSLMK FADQEGLTSS 51 VGEYNENTIQ QLLLPKIREL SDSIITLDSN FTRLNFIHES LADLNESLGS 101 LLYGIMSNSW CVEFSQAPHD IQDDLIAIKQ LKSLEDEKNN LVMELSNMER 151 GIKRKKDEQG ENDLAKASQN KQFNQPLFPS SQVRKYRSYD NRDKRKPSKI 201 GNNLQVENEE DYEDDTSSEA SFVLNPTNIG MSKSSQGHVT KTTRLNNNTN 251 SKLRRKSILH TIRNSIASGA DLPIENDNVV NLGDLHPNNR ISLGSGAARV 301 VNGPVTKNRN SMFSGRAERK PTESRHSVAK KTEKKINTRP PFR*

Amino Acid Sequence (or in FASTA format)

       1  MSEDKAKLGT TRSATEYRLS IGSAPTSRRS SMGESSSLMK FADQEGLTSS
      51  VGEYNENTIQ QLLLPKIREL SDSIITLDSN FTRLNFIHES LADLNESLGS
     101  LLYGIMSNSW CVEFSQAPHD IQDDLIAIKQ LKSLEDEKNN LVMELSNMER
     151  GIKRKKDEQG ENDLAKASQN KQFNQPLFPS SQVRKYRSYD NRDKRKPSKI
     201  GNNLQVENEE DYEDDTSSEA SFVLNPTNIG MSKSSQGHVT KTTRLNNNTN
     251  SKLRRKSILH TIRNSIASGA DLPIENDNVV NLGDLHPNNR ISLGSGAARV
     301  VNGPVTKNRN SMFSGRAERK PTESRHSVAK KTEKKINTRP PFR*

external links for Dam1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome
	DIP
	GeneMANIA
	YeastRC Mass Spec (Seattle)

References cited on this page View Complete Literature Guide for Dam1p

1)	Li Y, et al. (2002) The mitotic spindle is required for loading of the DASH complex onto the kinetochore. Genes Dev 16(2):183-97
2)	Cheeseman IM, et al. (2002) Phospho-regulation of kinetochore-microtubule attachments by the Aurora kinase Ipl1p. Cell 111(2):163-72
3)	Courtwright AM and He X (2002) Dam1 is the right one: phosphoregulation of kinetochore biorientation. Dev Cell 3(5):610-1
4)	Miranda JJ, et al. (2005) The yeast DASH complex forms closed rings on microtubules. Nat Struct Mol Biol 12(2):138-43
5)	Westermann S, et al. (2005) Formation of a dynamic kinetochore- microtubule interface through assembly of the Dam1 ring complex. Mol Cell 17(2):277-90
6)	Westermann S, et al. (2006) The Dam1 kinetochore ring complex moves processively on depolymerizing microtubule ends. Nature 440(7083):565-9