SRB5/YGR104C Protein Information

Standard Name	Srb5p 1
Systematic Name	Ygr104cp
Alias	Med18p 2
ORF Classification	Verified
Description	Subunit of the RNA polymerase II mediator complex; associates with core polymerase subunits to form the RNA polymerase II holoenzyme; essential for transcriptional regulation; required for proper termination of transcription for some genes; involved in telomere maintenance (1, 3, 4, 5, 6)
Name Description	Suppressor of RNA polymerase B 1

Experimental Data
Molecules/cell	1010 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	307
Molecular Weight (Da)	34,288
Isoelectric Point (pI)	4.69

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Srb5p (InterPro)
Physical Interactions	There are 298 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000005315D \| P32585 MIPS: YGR104C NCBI: 116667902 \| 116667904 \| 116667906 \| 116667908 \| 116667910 \| 116667912 \| 116667914 \| 116667916 \| 1323161 \| 172536 \| 345100888 \| 345100895 \| 345100902 \| 398365691 \| 410563053 \| 410563060 \| 417807 \| 459358310 \| 51013485 \| NP_011618.3 GenBank/EMBL/DDBJ: DAA08197.1 \| AY693017 \| L12028 \| Z72889

Amino Acid Sequence (or in FASTA format)
1 MVQQLSLFGS IGDDGYDLLI STLTTISGNP PLLYNSLCTV WKPNPSYDVE 51 NVNSRNQLVE PNRIKLSKEV PFSYLIDETM MDKPLNFRIL KSFTNDKIPL 101 NYAMTRNILH NTVPQVTNFN STNEDQNNSK HTEDTVNESR NSDDIIDVDM 151 DASPAPSNES CSPWSLQISD IPAAGNNRSV SMQTIAETII LSSAGKNSSV 201 SSLMNGLGYV FEFQYLTIGV KFFMKHGLIL ELQKIWQIEE AGNSQITSGG 251 FLLKAYINVS RGTDIDRINY TETALMNLKK ELQGYIELSV PDRQSMDSRV 301 AHGNILI*

Amino Acid Sequence (or in FASTA format)

       1  MVQQLSLFGS IGDDGYDLLI STLTTISGNP PLLYNSLCTV WKPNPSYDVE
      51  NVNSRNQLVE PNRIKLSKEV PFSYLIDETM MDKPLNFRIL KSFTNDKIPL
     101  NYAMTRNILH NTVPQVTNFN STNEDQNNSK HTEDTVNESR NSDDIIDVDM
     151  DASPAPSNES CSPWSLQISD IPAAGNNRSV SMQTIAETII LSSAGKNSSV
     201  SSLMNGLGYV FEFQYLTIGV KFFMKHGLIL ELQKIWQIEE AGNSQITSGG
     251  FLLKAYINVS RGTDIDRINY TETALMNLKK ELQGYIELSV PDRQSMDSRV
     301  AHGNILI*

external links for Srb5p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Aspergillus (AspGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CGD)	CYC2008 (complexes)	Pfam domains
Candida (CandidaDB)	Complexome
YGOB	DIP
YOGY	GeneMANIA

References cited on this page View Complete Literature Guide for Srb5p

1)	Thompson CM, et al. (1993) A multisubunit complex associated with the RNA polymerase II CTD and TATA-binding protein in yeast. Cell 73(7):1361-75
2)	Bourbon HM, et al. (2004) A unified nomenclature for protein subunits of mediator complexes linking transcriptional regulators to RNA polymerase II. Mol Cell 14(5):553-7
3)	Kim YJ, et al. (1994) A multiprotein mediator of transcriptional activation and its interaction with the C-terminal repeat domain of RNA polymerase II. Cell 77(4):599-608
4)	Kornberg RD (2005) Mediator and the mechanism of transcriptional activation. Trends Biochem Sci 30(5):235-9
5)	Askree SH, et al. (2004) A genome-wide screen for Saccharomyces cerevisiae deletion mutants that affect telomere length. Proc Natl Acad Sci U S A 101(23):8658-63
6)	Mukundan B and Ansari A (2011) Novel role for mediator complex subunit Srb5/Med18 in termination of transcription. J Biol Chem 286(43):37053-7
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41