DBF2/YGR092W Protein Information Help

Standard Name Dbf2p
Systematic Name Ygr092wp
ORF Classification Verified
Description Ser/Thr kinase involved in transcription and stress response; functions as part of a network of genes in exit from mitosis; localization is cell cycle regulated; activated by Cdc15p during the exit from mitosis; also plays a role in regulating the stability of SWI5 and CLB2 mRNAs; phosphorylates Chs2p to regulate primary septum formation and Hof1p to regulate cytokinesis; DBF2 has a paralog, DBF20, that arose from the whole genome duplication (1, 2, 3, 4, 5, 6, 7)
Name Description DumbBell Forming 8
Experimental Data
Molecules/cell 3500 9
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 572
Molecular Weight (Da) 66,148
Isoelectric Point (pI) 9.27

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Dbf2p (InterPro)
Physical Interactions There are 176 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI0000128EE4 | P22204
MIPS: YGR092W
NCBI: 1323137 | 1706307 | 171378 | 398365629 | NP_011606.3
GenBank/EMBL/DDBJ: DAA08185.1 | M34146 | Z72877
External Classifications EC: 2.7.11.1 [Non-specific serine/threonine protein kinase]
Amino Acid Sequence (or in FASTA format)
 
       1  MLSKSEKNVD LLAGNMSNLS FDGHGTPGGT GLFPNQNITK RRTRPAGIND
      51  SPSPVKPSFF PYEDTSNMDI DEVSQPDMDV SNSPKKLPPK FYERATSNKT
     101  QRVVSVCKMY FLEHYCDMFD YVISRRQRTK QVLEYLQQQS QLPNSDQIKL
     151  NEEWSSYLQR EHQVLRKRRL KPKNRDFEMI TQVGQGGYGQ VYLARKKDTK
     201  EVCALKILNK KLLFKLNETK HVLTERDILT TTRSEWLVKL LYAFQDLQSL
     251  YLAMEFVPGG DFRTLLINTR CLKSGHARFY ISEMFCAVNA LHDLGYTHRD
     301  LKPENFLIDA KGHIKLTDFG LAAGTISNER IESMKIRLEK IKDLEFPAFT
     351  EKSIEDRRKM YNQLREKEIN YANSMVGSPD YMALEVLEGK KYDFTVDYWS
     401  LGCMLFESLV GYTPFSGSST NETYDNLRRW KQTLRRPRQS DGRAAFSDRT
     451  WDLITRLIAD PINRLRSFEH VKRMSYFADI NFSTLRSMIP PFTPQLDSET
     501  DAGYFDDFTS EADMAKYADV FKRQDKLTAM VDDSAVSSKL VGFTFRHRNG
     551  KQGSSGILFN GLEHSDPFST FY*

external links for Dbf2p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily YPL+
Ashbya (AGD) BOND GPMdb (Mass Spec.) YeastGFP
YGOB BioPIXIE MIPS YeastRC Public Image Repository
YOGY CYC2008 (complexes) Pfam domains

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Dbf2p
1) Toyn JH and Johnston LH  (1994) The Dbf2 and Dbf20 protein kinases of budding yeast are activated after the metaphase to anaphase cell cycle transition. EMBO J 13(5):1103-13
2) Frenz LM, et al.  (2000) The budding yeast Dbf2 protein kinase localises to the centrosome and moves to the bud neck in late mitosis. J Cell Sci 113 Pt 19():3399-408
3) Visintin R and Amon A  (2001) Regulation of the mitotic exit protein kinases Cdc15 and Dbf2. Mol Biol Cell 12(10):2961-74
4) Byrne KP and Wolfe KH  (2005) The Yeast Gene Order Browser: combining curated homology and syntenic context reveals gene fate in polyploid species. Genome Res 15(10):1456-61
5) Trcek T, et al.  (2011) Single-molecule mRNA decay measurements reveal promoter- regulated mRNA stability in yeast. Cell 147(7):1484-97
6) Oh Y, et al.  (2012) Mitotic exit kinase Dbf2 directly phosphorylates chitin synthase Chs2 to regulate cytokinesis in budding yeast. Mol Biol Cell 23(13):2445-56
7) Meitinger F, et al.  (2013) Dual function of the NDR-kinase Dbf2 in the regulation of the F-BAR protein Hof1 during cytokinesis. Mol Biol Cell 24(9):1290-304
8) Johnston LH and Thomas AP  (1982) The isolation of new DNA synthesis mutants in the yeast Saccharomyces cerevisiae. Mol Gen Genet 186(3):439-44
9) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41