SPT4/YGR063C Protein Information 
| Standard Name | Spt4p 1, 2 |
|---|---|
| Systematic Name | Ygr063cp |
| ORF Classification | Verified |
| Description | Component of the universally conserved Spt4/5 complex (DSIF complex); the complex has multiple roles in concert with RNA polymerases I and II, including regulation of transcription elongation, RNA processing, quality control, and transcription-coupled DNA repair; Spt4p also localizes to kinetochores and heterochromatin and affects chromosome dynamics and silencing; required for transcription through lengthy trinucleotide repeats in ORFs or non-protein coding regions (10, 11, 3, 4, 5, 6, 7, 8, 9) |
| Name Description | SuPpressor of Ty's 1 |
| Experimental Data | |
|---|---|
| Molecules/cell | 4490 12 |
| Click on image for expanded interactive view |
|---|
| Post-translational Modifications | PhosphoGRID | PhosphoPep Database |
|---|---|
| Domains/motifs | See the graphical view and list of proteins that share domains/motifs in common with Spt4p (InterPro) |
| Physical Interactions | There are 86 total physical interactions (BioGRID) |
| Homologs | PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer |
| External Sequence Databases |
EBI: UPI0000053199 | P32914 MIPS: YGR063C NCBI: 112362848 | 112362850 | 112362852 | 112362854 | 112362856 | 112362858 | 112362860 | 112362862 | 112362864 | 112362866 | 112362868 | 112362870 | 112362872 | 119389383 | 1323083 | 295667 | 398365457 | 417803 | 51013631 | 71064049 | NP_011577.3 GenBank/EMBL/DDBJ: DAA08159.1 | AM296426 | AM296427 | AM296428 | AM296429 | AM296430 | AM296431 | AM296432 | AM296433 | AM296434 | AM296435 | AM296436 | AM296437 | AM296438 | AY693090 | DQ115391 | M83672 | Z72848 |
external links for Spt4p
| Homologs | Interaction Resources | Protein databases/Other | Localization Resources |
|---|---|---|---|
| BLASTP (NCBI) | BioGRID | SCOP Superfamily | YPL+ |
| Ashbya (AGD) | BOND | GPMdb (Mass Spec.) | YeastGFP |
| Aspergillus (AspGD) | BioPIXIE | MIPS | YeastRC Public Image Repository |
| Candida (CGD) | CYC2008 (complexes) | Pfam domains | |
| Candida (CandidaDB) | Complexome | ||
| YGOB | DIP | ||
| YOGY | GeneMANIA |
References cited on this page View Complete Literature Guide for Spt4p
| 1) | Winston F, et al. (1984) Mutations affecting Ty-mediated expression of the HIS4 gene of Saccharomyces cerevisiae. Genetics 107(2):179-97 |
| 2) | Fassler, J. and Winston, F. (1989) Personal Communication, Mortimer Map Edition 10 |
| 3) | Lindstrom DL, et al. (2003) Dual roles for Spt5 in pre-mRNA processing and transcription elongation revealed by identification of Spt5-associated proteins. Mol Cell Biol 23(4):1368-78 |
| 4) | Rondon AG, et al. (2003) Molecular evidence for a positive role of Spt4 in transcription elongation. EMBO J 22(3):612-20 |
| 5) | Crotti LB and Basrai MA (2004) Functional roles for evolutionarily conserved Spt4p at centromeres and heterochromatin in Saccharomyces cerevisiae. EMBO J 23(8):1804-14 |
| 6) | Schneider DA, et al. (2006) RNA polymerase II elongation factors Spt4p and Spt5p play roles in transcription elongation by RNA polymerase I and rRNA processing. Proc Natl Acad Sci U S A 103(34):12707-12 |
| 7) | Ding B, et al. (2010) The C-terminal repeat domain of Spt5 plays an important role in suppression of Rad26-independent transcription coupled repair. J Biol Chem 285(8):5317-26 |
| 8) | Shen Z, et al. (2010) Cotranscriptional recruitment of She2p by RNA pol II elongation factor Spt4-Spt5/DSIF promotes mRNA localization to the yeast bud. Genes Dev 24(17):1914-26 |
| 9) | Anderson SJ, et al. (2011) The transcription elongation factor Spt5 influences transcription by RNA polymerase I positively and negatively. J Biol Chem 286(21):18816-24 |
| 10) | Lepore N and Lafontaine DL (2011) A Functional Interface at the rDNA Connects rRNA Synthesis, Pre-rRNA Processing and Nucleolar Surveillance in Budding Yeast. PLoS One 6(9):e24962 |
| 11) | Liu CR, et al. (2012) Spt4 is selectively required for transcription of extended trinucleotide repeats. Cell 148(4):690-701 |
| 12) | Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41 |
