TIM21/YGR033C Protein Information

Standard Name	Tim21p 1
Systematic Name	Ygr033cp
ORF Classification	Verified
Description	Nonessential subunit of the Translocase of the Inner Mitochondrial membrane (TIM23 complex); interacts with the Translocase of the Outer Mitochondrial membrane (TOM complex) and with respiratory enzymes; may regulate TIM23 complex activity (1, 2, 3, 4)
Name Description	Translocase of the Inner Mitochondrial membrane 1

Experimental Data
Molecules/cell	3770 5

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	239
Molecular Weight (Da)	27,205
Isoelectric Point (pI)	10.96

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Tim21p (InterPro)
Transmembrane Domains	There are 1 total predicted transmembrane domains (TMHMM)
Physical Interactions	There are 64 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000013AF32 \| P53220 MIPS: YGR033C NCBI: 122919706 \| 1323014 \| 1723658 \| 398365261 \| 71064020 \| NP_011547.3 GenBank/EMBL/DDBJ: DAA08130.1 \| DQ115390 \| Z72818

Amino Acid Sequence (or in FASTA format)
1 MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS 51 GKKDDKTRNK PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF 101 SPSGDTQLFN RAVSMVEKNK DIRSLLQCDD GITGKERLKA YGELITNDKW 151 TRNRPIVSTK KLDKEGRTHH YMRFHVESKK KIALVHLEAK ESKQNYQPDF 201 INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD*

Amino Acid Sequence (or in FASTA format)

       1  MSSSLPRSLL RLGHRKPLFP RYNTFVNSSV ITHTSLLRTR LYSNGTGATS
      51  GKKDDKTRNK PKPLWPQVKS ASTFTFSGIL VIGAVGISAI VIYLILSELF
     101  SPSGDTQLFN RAVSMVEKNK DIRSLLQCDD GITGKERLKA YGELITNDKW
     151  TRNRPIVSTK KLDKEGRTHH YMRFHVESKK KIALVHLEAK ESKQNYQPDF
     201  INMYVDVPGE KRYYLIKPKL HPVSNSKGFL GIRWGPRKD*

external links for Tim21p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
Candida (CGD)	BioPIXIE	MIPS	YeastRC Public Image Repository
Candida (CandidaDB)	CYC2008 (complexes)	Pfam domains
YGOB	Complexome	YeastRC Structure Prediction (Seattle)
YOGY	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Tim21p

1)	Chacinska A, et al. (2005) Mitochondrial presequence translocase: switching between TOM tethering and motor recruitment involves Tim21 and Tim17. Cell 120(6):817-29
2)	Mokranjac D, et al. (2005) Role of Tim21 in mitochondrial translocation contact sites. J Biol Chem 280(25):23437-40
3)	van der Laan M, et al. (2006) A role for Tim21 in membrane-potential-dependent preprotein sorting in mitochondria. Curr Biol 16(22):2271-6
4)	Popov-Celeketic D, et al. (2008) Active remodelling of the TIM23 complex during translocation of preproteins into mitochondria. EMBO J 27(10):1469-80
5)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41