CUL3/YGR003W Protein Information Help

Standard Name Cul3p 1
Systematic Name Ygr003wp
ORF Classification Verified
Description Ubiquitin-protein ligase, forms a complex with Elc1p that polyubiquitylates monoubiquitylated RNA polymerase II to trigger its proteolysis; cullin family member with similarity to Cdc53p and human CUL3 (1, 2, 3, 4) Also known as: CULB
Name Description CULlin 5
Gene Product CULLIN B
Experimental Data
Molecules/cell 172 6
Predicted Sequence Formatted Sequence or sequence in FASTA format
Length (a.a.) 744
Molecular Weight (Da) 86,114
Isoelectric Point (pI) 6.18

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Post-translational Modifications PhosphoGRID | PhosphoPep Database
Domains/motifs See the graphical view and list of proteins that share domains/motifs in common with Cul3p (InterPro)
Physical Interactions There are 12 total physical interactions (BioGRID)
Homologs PDB Homologs | BLASTP | BLASTP v. fungi | Fungal Alignment | Synteny Viewer
External Sequence Databases EBI: UPI000013AF1B | P53202
MIPS: YGR003W
NCBI: 1322957 | 1723641 | 37362912 | 6321440 | NP_011517.1
GenBank/EMBL/DDBJ: DAA08101.1 | AY387706 | Z72788
Amino Acid Sequence (or in FASTA format)
 
       1  MITNKKIKIS VPEKLGLSEE SFEESWETVK YAIDHIYSDD MADLSFEQVY
      51  KTIYTIVLNK KGPILYNRLK DYLIQKLALL RETIVKDNTH DYEFLGTMAR
     101  LWEVQCHCFK ITGDLMMYMD KVYCKPNRCM EVYDMCLDLF RIEILQKCSS
     151  SLISALISDI ERIRNLGSVD SEHTSLWKVL IGMMETLHDN RDSFFLTDFE
     201  PVLISATEEY YNKAIDIELL TPIESLEKIR KLRQFESMLD SSFLNVDSHN
     251  KLKTVLENVL IWGKLSDIIE DLTHEAMVIS NGKLLQEIYD LSSEEKYRVT
     301  VIESIKSYIN KNAINIPFNE GNRKKGQNAI TWSSEIVELY RSQHSFLESI
     351  DFGSVRLNNL TGDVSNAILG DVFSMYFSKE GALPSEYLST YVDHCMKRTK
     401  EKDAEIVKIK QDLLDSTKLI GLLTEKDIFE KIYKKQLSRR LLQQRSLVEI
     451  EKWMVQMIKK VLGTFFTSKL EIMLRDISLS SEMYQAFQHS TINSIEYLSF
     501  APQVLTRTSW PFQSTNPIDE GISLPPRMSQ ILAGFEGYYS LKYKERVLKW
     551  AHHLSVIEIG CQFNSGYYEI SFSVYAGVIF LLFEDYEELT LGEIYELTHI
     601  PIDDVKSLVM SMSTIPRCKI LKKSSSSGNM KFSVNYFFSS PNRKVKVPVI
     651  ACPLPSQKSD NLATASSVDT YDNEIVMELS AIIVRIMKTE GKLSHQQLLE
     701  RTTKRTQSRL SLTPSILKRS IQLLIEKEYI QRNADDPSYY HYLS*

external links for Cul3p
Homologs Interaction Resources Protein databases/Other Localization Resources
BLASTP (NCBI) BioGRID SCOP Superfamily Organelle DB
Ashbya (AGD) BOND GPMdb (Mass Spec.) YPL+
YGOB BioPIXIE MIPS YeastGFP
YOGY CYC2008 (complexes) Pfam domains YeastRC Public Image Repository

Complexome YeastRC Structure Prediction (Seattle)

DIP


GeneMANIA

References cited on this page View Complete Literature Guide for Cul3p
1) Michel JJ, et al.  (2003) A role for Saccharomyces cerevisiae Cul8 ubiquitin ligase in proper anaphase progression. J Biol Chem 278(25):22828-37
2) Lammer D, et al.  (1998) Modification of yeast Cdc53p by the ubiquitin-related protein rub1p affects function of the SCFCdc4 complex. Genes Dev 12(7):914-26
3) Ribar B, et al.  (2007) ELA1 and CUL3 are required along with ELC1 for RNA polymerase II polyubiquitylation and degradation in DNA-damaged yeast cells. Mol Cell Biol 27(8):3211-6
4) Harreman M, et al.  (2009) Distinct ubiquitin ligases act sequentially for RNA polymerase II polyubiquitylation. Proc Natl Acad Sci U S A 106(49):20705-10
5) Ohta T, et al.  (1999) ROC1, a homolog of APC11, represents a family of cullin partners with an associated ubiquitin ligase activity. Mol Cell 3(4):535-41
6) Ghaemmaghami S, et al.  (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41