FZF1/YGL254W Protein Information

Standard Name	Fzf1p 1
Systematic Name	Ygl254wp
Alias	Nrc299p , Rsu1p 2 , Sul1p 3
ORF Classification	Verified
Description	Transcription factor involved in sulfite metabolism; sole identified regulatory target is SSU1; overexpression suppresses sulfite-sensitivity of many unrelated mutants due to hyperactivation of SSU1, contains five zinc fingers; protein abundance increases in response to DNA replication stress (1, 4, 5, 6)
Name Description	Five Zinc Fingers 1

Experimental Data
Molecules/cell	2120 7

Predicted Sequence	Formatted Sequence or sequence in FASTA format
Length (a.a.)	299
Molecular Weight (Da)	33,994
Isoelectric Point (pI)	7.77

Click on image for expanded interactive view

Post-translational Modifications	PhosphoGRID \| PhosphoPep Database
Domains/motifs	See the graphical view and list of proteins that share domains/motifs in common with Fzf1p (InterPro)
Physical Interactions	There are 12 total physical interactions (BioGRID)
Homologs	PDB Homologs \| BLASTP \| BLASTP v. fungi \| Fungal Alignment \| Synteny Viewer
External Sequence Databases	EBI: UPI000012AE18 \| P32805 MIPS: YGL254W NCBI: 1150585 \| 1322933 \| 1326037 \| 1730142 \| 460066 \| 63028875 \| 63028877 \| 63028881 \| 63028883 \| 63028887 \| 63028889 \| 63028891 \| 63028893 \| 63028895 \| 63028897 \| 63028899 \| 63028901 \| 63028903 \| 63028905 \| 63028907 \| 63028909 \| 6321183 \| NP_011260.1 GenBank/EMBL/DDBJ: DAA07865.1 \| AY949902 \| AY949903 \| AY949905 \| AY949906 \| AY949908 \| AY949909 \| AY949910 \| AY949911 \| AY949912 \| AY949913 \| AY949914 \| AY949915 \| AY949916 \| AY949917 \| AY949918 \| AY949919 \| X67787 \| X78104 \| X94357 \| Z72776

Amino Acid Sequence (or in FASTA format)
1 MTDIGRTKSR NYKCSFDGCE KVYNRPSLLQ QHQNSHTNQK PYHCDEPGCG 51 KKFIRPCHLR VHKWTHSQIK PKACTLCQKR FVTNQQLRRH LNSHERKSKL 101 ASRIDRKHEG VNANVKAELN GKEGGFDPKL PSGSPMCGEE FSQGHLPGYD 151 DMQVLQCPYK SCQKVTSFND DLINHMLQHH IASKLVVPSG DPSLKESLPT 201 SEKSSSTDTT SIPQLSFSTT GTSSSESVDS TTAQTPTDPE SYWSDNRCKH 251 SDCQELSPFA SVFDLIDHYD HTHAFIPETL VKYSYIHLYK PSVWDLFEY*

Amino Acid Sequence (or in FASTA format)

       1  MTDIGRTKSR NYKCSFDGCE KVYNRPSLLQ QHQNSHTNQK PYHCDEPGCG
      51  KKFIRPCHLR VHKWTHSQIK PKACTLCQKR FVTNQQLRRH LNSHERKSKL
     101  ASRIDRKHEG VNANVKAELN GKEGGFDPKL PSGSPMCGEE FSQGHLPGYD
     151  DMQVLQCPYK SCQKVTSFND DLINHMLQHH IASKLVVPSG DPSLKESLPT
     201  SEKSSSTDTT SIPQLSFSTT GTSSSESVDS TTAQTPTDPE SYWSDNRCKH
     251  SDCQELSPFA SVFDLIDHYD HTHAFIPETL VKYSYIHLYK PSVWDLFEY*

external links for Fzf1p

Homologs	Interaction Resources	Protein databases/Other	Localization Resources
BLASTP (NCBI)	BioGRID	SCOP Superfamily	YPL+
Ashbya (AGD)	BOND	GPMdb (Mass Spec.)	YeastGFP
YGOB	BioPIXIE	MIPS	YeastRC Public Image Repository
YOGY	CYC2008 (complexes)	Pfam domains
	Complexome	YeastRC Structure Prediction (Seattle)
	DIP
	GeneMANIA

References cited on this page View Complete Literature Guide for Fzf1p

1)	Breitwieser W, et al. (1993) Identification of a gene encoding a novel zinc finger protein in Saccharomyces cerevisiae. Yeast 9(5):551-6
2)	Park H, et al. (1999) Use of sulfite resistance in Saccharomyces cerevisiae as a dominant selectable marker. Curr Genet 36(6):339-44
3)	Casalone E, et al. (1994) Cloning and characterization of a sulphite-resistance gene of Saccharomyces cerevisiae. Yeast 10(8):1101-10
4)	Avram D and Bakalinsky AT (1996) Multicopy FZF1 (SUL1) suppresses the sulfite sensitivity but not the glucose derepression or aberrant cell morphology of a grr1 mutant of Saccharomyces cerevisiae. Genetics 144(2):511-21
5)	Avram D, et al. (1999) Fzf1p of Saccharomyces cerevisiae is a positive regulator of SSU1 transcription and its first zinc finger region is required for DNA binding. Yeast 15(6):473-80
6)	Tkach JM, et al. (2012) Dissecting DNA damage response pathways by analysing protein localization and abundance changes during DNA replication stress. Nat Cell Biol 14(9):966-76
7)	Ghaemmaghami S, et al. (2003) Global analysis of protein expression in yeast. Nature 425(6959):737-41